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3.2.1.25: beta-mannosidase

This is an abbreviated version!
For detailed information about beta-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.25

Reaction

Manbeta1-4GlcNAcbeta(1-4)GlcNAcbeta-Asn
+
H2O
=
GlcNAc-(1-4)-beta-GlcNAc-Asn
+
beta-D-mannopyranose

Synonyms

beta-D-mannosidase, beta-MAN, beta-mannosidase, beta-mannosidase 2A, beta-mannoside mannohydrolase, betaMANNOS1, BglB, CmMan5, CmMan5a, exo beta-mannanase, exo-beta-D-mannanase, GH2 beta-mannosidase, GM-1, GM-2, HvBII, Man2A, ManB, mannanase, mannase, mannosidase 5A, mannosidase, beta-, OT-1, PH0501, TM1624

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.25 beta-mannosidase

Purification

Purification on EC 3.2.1.25 - beta-mannosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
beta-mannanase I and II
-
Mono S HR 5/5 column chromatography and Superdex 200 gel filtration
-
partial
Q-Sepharose column chromatography, SP-Sepharose column chromatography, and chromatofocusing on Polybuffer Exchanger PBE-94
-
recombinant
-
recombinant enzyme
TALON immobilized metal ion affinity chromatography, Q12 column chromatography, and S200 gel filtration
-
two forms of enzyme: beta-mannosidase A and B
-