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Literature summary for 3.2.1.25 extracted from
- Purification and characterisation of extremely thermostable beta-mannanase, beta-mannosidase, and alpha-galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana 5068 (1997), Appl. Environ. Microbiol., 63, 169-177.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
gel filtration | Thermotoga neapolitana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga neapolitana | - |
5068 | - |
| Thermotoga neapolitana 5068 | - |
5068 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga neapolitana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-beta-D-mannoside + H2O | - |
Thermotoga neapolitana | p-nitrophenol + alpha-D-mannose | - |
? |  |
| p-nitrophenyl-beta-D-mannoside + H2O | - |
Thermotoga neapolitana 5068 | p-nitrophenol + alpha-D-mannose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 200000-220000, SDS-PAGE | Thermotoga neapolitana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 87 | - |
- |
Thermotoga neapolitana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 90 | 70°C: about 40% of maximal activity, 90°C: about 80% of maximal activity | Thermotoga neapolitana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
half-life: 18 h | Thermotoga neapolitana |
| 90 | - |
half-life: 42 min | Thermotoga neapolitana |
| 98 | - |
half-life: 2 min | Thermotoga neapolitana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Thermotoga neapolitana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: about 35% of maximal activity, pH 9.0: about 40% of maximal activity | Thermotoga neapolitana |
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