3.2.1.25: beta-mannosidase

This is an abbreviated version!
For detailed information about beta-mannosidase, go to the full flat file.

Reaction

Synonyms

beta-D-mannosidase, beta-MAN, beta-mannosidase, beta-mannosidase 2A, beta-mannoside mannohydrolase, betaMANNOS1, BglB, CmMan5, CmMan5a, exo beta-mannanase, exo-beta-D-mannanase, GH2 beta-mannosidase, GM-1, GM-2, HvBII, Man2A, ManB, mannanase, mannase, mannosidase 5A, mannosidase, beta-, OT-1, PH0501, TM1624

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.25 beta-mannosidase

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Results	in table
16	Activating Compound
4311	AA Sequence
4	Application
1	CAS Registry Number
15	Cloned(Commentary)
8	Crystallization (Commentary)
296	Disease/ Diagnostics
2	Expression
2	General Information
7	General Stability
97	Inhibitors
20	kcat/KM [mM/s]
24	Ki Value [mM]
92	KM Value [mM]
15	Localization
5	Metals/Ions
52	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
114	Organism
1	Oxidation Stability
1	Pathway
35	PDB ID
38	pH Optimum
6	pH Range
20	pH Stability
7	pI Value
14	Posttranslational Modification
33	Protein Variants
37	Purification (Commentary)
2	Reaction
1	Reaction Type
91	Reference
52	Source Tissue
32	Specific Activity [micromol/min/mg]
6	Storage Stability
197	Substrates and Products (Substrate)
35	Subunits
36	Synonyms
1	Systematic Name
20	Temperature Optimum [°C]
6	Temperature Range [°C]
40	Temperature Stability [°C]
45	Turnover Number [1/s]

pH Stability

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pH Stability on EC 3.2.1.25 - beta-mannosidase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10

Rattus norvegicus

-

4°C, 1 h, little loss of activity

136045

2.5 - 7.5

Aspergillus niger

-

37°C, 2 h, stable

136039

2.8 - 9

Polyporus sulfureus

-

25°C, 1 h, stable

136041

3.5

Tremella fuciformis

-

rapid loss of activity

136038

3.5 - 6

Trichoderma reesei

-

stable

136058

4 - 6

Aspergillus niger

-

high stability at

136059

4.5

3 entries

4.5 - 7

Lactuca sativa

-

stable

136034

5 - 5.5

Tremella fuciformis

-

37°C, 1 h, stable

136038

5 - 7

Lissachatina fulica

-

55°C, 5 min, pH 5-7, 10-20% loss of activity

136047

5 - 8

Cyamopsis tetragonoloba

-

40°C, 30 min, stable up to

136030

5 - 9

Thermotoga maritima

Q9X1V9

70°C, 30 minm, retention of 80% of maximal activity between the range of pH 5.0 and 8.0

748422

5.9

Thermoascus aurantiacus

-

exhibits highest pH stability at pH 5.9 after 5 h incubation at 50°C

679572

6 - 10

Lactuca sativa

-

unstable below pH 6.0 and above pH 10.0

136034

6.5 - 8

Bacillus sp. (in: Bacteria)

-

40°C, 30 min, stable. Unstable below pH 6.5 and above pH 8.0

136031

6.5 - 9

Thermotoga neapolitana

-

pH 6.5: about 35% of maximal activity, pH 9.0: about 40% of maximal activity

136061

7

Helix pomatia

-

unstable above pH 7

136029

7.5 - 9

Homo sapiens

-

20°C, 60 min, stable

136027

4.5

Athelia rolfsii

-

most stable at

136060

4.5

Helix pomatia

-

stable to extended incubation at 45°C

136029

4.5

Lissachatina fulica

-

55°C, 5 min, 40% loss of activity, at pH-values lower than pH 4.5, 80% loss of activity

136047