3.2.1.133: glucan 1,4-alpha-maltohydrolase

This is an abbreviated version!
For detailed information about glucan 1,4-alpha-maltohydrolase, go to the full flat file.

Reaction

Synonyms

alpha-amylase, AmyB, BbmA, BSMA, BSTA, BTMA, CAZy-GH13, glucan 1,4-alpha-maltohydrolase, glucan-1,4-alpha-maltohydrolase, Gt-MamyIII, LGMA, MAase, MABS, MAG1, maltogenase L, maltogenic alpha-amylase, maltogenic amylase, maltose-forming alpha-amylase, MAmy, MAUS149, More, NM319, NM326, NM398, NM404, NM447, Novamyl, PSMA, Smar_0613, SMMA, TCMA, Thermus maltogenic amylase, ThMA, TK4MA

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.133 glucan 1,4-alpha-maltohydrolase

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Results	in table
8	Activating Compound
70	AA Sequence
35	Application
1	CAS Registry Number
36	Cloned(Commentary)
7	Crystallization (Commentary)
3	General Information
3	General Stability
69	Inhibitors
15	kcat/KM [mM/s]
1	Ki Value [mM]
57	KM Value [mM]
13	Localization
22	Metals/Ions
39	Molecular Weight [Da]
34	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
106	Organism
5	Pathway
4	PDB ID
22	pH Optimum
12	pH Range
6	pH Stability
1	pI Value
95	Protein Variants
32	Purification (Commentary)
1	Reaction
7	Reaction Type
83	Reference
3	Source Tissue
48	Specific Activity [micromol/min/mg]
253	Substrates and Products (Substrate)
49	Subunits
134	Synonyms
1	Systematic Name
40	Temperature Optimum [°C]
15	Temperature Range [°C]
39	Temperature Stability [°C]
88	Turnover Number [1/s]

KM Value

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KM Value on EC 3.2.1.133 - glucan 1,4-alpha-maltohydrolase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.426 - 1.08

acarbose

4 entries

0.43

alpha-cyclodextrin

Staphylothermus marinus

A3DM60

pH and temperature not specified in the publication

732084

0.05 - 2.03

beta-cyclodextrin

13 entries

2.68

gamma-cyclodextrin

Staphylothermus marinus

A3DM60

pH and temperature not specified in the publication

732084

1.5 - 9.21

maltoheptaose

3 entries

3.8 - 7.53

maltohexaose

3 entries

3.5 - 21.2

maltopentaose

5 entries

63.43 - 427

maltose

2 entries

1.6 - 18.8

maltotetraose

5 entries

1.13 - 35.3

maltotriose

11 entries

4.1 - 13

soluble starch

2 entries

-

additional information

additional information

7 entries

-

0.426

acarbose

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, wild-type

646800

0.545

acarbose

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, mutant E332D

646800

1

acarbose

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, mutant E332Q

646800

1.08

acarbose

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, mutant E332H

646800

0.05

beta-cyclodextrin

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, variant DM

646805

0.09

beta-cyclodextrin

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, variant 4B74

646805

0.128

beta-cyclodextrin

Bacillus thermoalkalophilus

-

-

663819

0.16

beta-cyclodextrin

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, wild-type and variants 3C71, 4B78 and 4A48

646805

0.17

beta-cyclodextrin

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, variants 1B76, 1B100

646805

0.174

beta-cyclodextrin

Thermus sp.

-

pH 6.0, 60°C, wild-type

646803

0.19

beta-cyclodextrin

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, variant 2A39

646805

0.263

beta-cyclodextrin

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, wild-type

646801

0.308

beta-cyclodextrin

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, wild-type, 1 M KCl

646801

0.36

beta-cyclodextrin

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, truncated mutant ThMA DELTA124, 1M KCl

646801

0.461

beta-cyclodextrin

Thermus sp.

-

pH 6.0, 60°C, hydrolysis, truncated mutant ThMA- DELTA124

646801

1.48

beta-cyclodextrin

Staphylothermus marinus

A3DM60

pH and temperature not specified in the publication

732084

2.03

beta-cyclodextrin

Thermus sp.

-

pH 6.0, 60°C, mutant W47A

646803

1.5

maltoheptaose

Thermoplasma volcanium

-

kinetic parameters for C14-labelled maltooligosaccharides

678461

8.18

maltoheptaose

Thermus sp.

-

50°C, pH 6.0, mutant enzyme A290I

664208

9.21

maltoheptaose

Thermus sp.

-

50°C, pH 6.0, wild-type enzyme

664208

3.8

maltohexaose

Thermoplasma volcanium

-

kinetic parameters for C14-labelled maltooligosaccharides

678461

7.16

maltohexaose

Thermus sp.

-

50°C, pH 6.0, wild-type enzyme

664208

7.53

maltohexaose

Thermus sp.

-

50°C, pH 6.0, mutant enzyme A290I

664208

3.5

maltopentaose

Thermus sp.

-

50°C, pH 6.0, mutant enzyme A290I

664208

3.97

maltopentaose

Thermus sp.

-

50°C, pH 6.0, wild-type enzyme

664208

4.4

maltopentaose

Thermoplasma volcanium

-

kinetic parameters for C14-labelled maltooligosaccharides

678461

16.2

maltopentaose

Thermococcus sp.

I3ZTN9

pH 5.0, 85°C

727388

21.2

maltopentaose

Pyrococcus sp.

I3RE04

pH 5.0, 90°C

724097

63.43

maltose

Thermus sp.

-

50°C, pH 6.0, wild-type enzyme

664208

427

maltose

Thermus sp.

-

50°C, pH 6.0, mutant enzyme A290I

664208

1.6

maltotetraose

Thermoplasma volcanium

-

kinetic parameters for C14-labelled maltooligosaccharides

678461

2.95

maltotetraose

Thermus sp.

-

50°C, pH 6.0, mutant enzyme A290I

664208

3.38

maltotetraose

Thermus sp.

-

50°C, pH 6.0, wild-type enzyme

664208

18.2

maltotetraose

Thermococcus sp.

I3ZTN9

pH 5.0, 85°C

727388

18.8

maltotetraose

Pyrococcus sp.

I3RE04

pH 5.0, 90°C

724097

1.13

maltotriose

Thermus sp.

-

50°C, pH 6.0, wild-type enzyme

664208

2.52

maltotriose

Thermus sp.

-

50°C, pH 6.0, mutant enzyme A290I

664208

3.6

maltotriose

Thermoplasma volcanium

-

kinetic parameters for C14-labelled maltooligosaccharides

678461

4.25

maltotriose

Thermococcus sp.

I3ZTN9

pH 5.0, 85°C

727388

8.4

maltotriose

Pyrococcus sp.

I3RE04

pH 5.0, 90°C

724097

12.3

maltotriose

Geobacillus stearothermophilus

P19531

pH 5.5, 60°C, wild-type enzyme

751152

16.4

maltotriose

Geobacillus stearothermophilus

P19531

pH 5.5, 60°C, mutant enzyme W177F

751152

22

maltotriose

Geobacillus stearothermophilus

P19531

pH 5.5, 60°C, mutant enzyme W177Y

751152

28

maltotriose

Geobacillus stearothermophilus

P19531

pH 5.5, 60°C, mutant enzyme W177L

751152

31.7

maltotriose

Geobacillus stearothermophilus

P19531

pH 5.5, 60°C, mutant enzyme W177N

751152

35.3

maltotriose

Geobacillus stearothermophilus

P19531

pH 5.5, 60°C, mutant enzyme W177S

751152

4.1

soluble starch

Thermoplasma volcanium

-

estimated for monomeric conformation, in presence of 1 M NaCl

678461

-

13

soluble starch

Thermoplasma volcanium

-

estimated for the high oligomer conformation

678461

-

additional information

additional information

Thermus sp.

-

Km 5.34 mg mL-1, starch as substrate, pH 6.0, 60°C, hydrolysis, truncated mutant ThMA-DELTA124

646801

-

additional information

additional information

Thermus sp.

-

Km 49.7 mg/ml, starch as substrate, pH 6.0, 60°C, wild-type, 1 M KCl

646801

-

additional information

additional information

Thermus sp.

-

Km 7.72 mg/ml, starch as substrate, pH 6.0, 60°C, truncated mutant ThMA-DELTA124, 1M KCl

646801

-

additional information

additional information

Thermus sp.

-

Km 131 mg/ml, starch as substrate, mutant W47A

646803

-

additional information

additional information

Thermus sp.

-

Km 73.5 mg/ml, starch as substrate, wild-type

646803

-

additional information

additional information

Thermus sp.

-

Km 61.0 mg/ml, starch as substrate, pH 6.0, 60°C, hydrolysis, wild-type

646801

-

additional information

additional information

Thermus sp.

-

lower affinity for binding of amylopectin but higher affinity for amylose in mutant enzymes demonstrated, sterospecific substrate binding properties of triple mutant enzyme determined by molecular modelling

678795

-