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3.2.1.133: glucan 1,4-alpha-maltohydrolase

This is an abbreviated version!
For detailed information about glucan 1,4-alpha-maltohydrolase, go to the full flat file.

Word Map on EC 3.2.1.133

Reaction

(Glcalpha(1-4))n
+
H2O
=
(Glcalpha(1-4))n-2
 +
alpha-maltose

Synonyms

alpha-amylase, AmyB, BbmA, BSMA, BSTA, BTMA, CAZy-GH13, glucan 1,4-alpha-maltohydrolase, glucan-1,4-alpha-maltohydrolase, Gt-MamyIII, LGMA, MAase, MABS, MAG1, maltogenase L, maltogenic alpha-amylase, maltogenic amylase, maltose-forming alpha-amylase, MAmy, MAUS149, More, NM319, NM326, NM398, NM404, NM447, Novamyl, PSMA, Smar_0613, SMMA, TCMA, Thermus maltogenic amylase, ThMA, TK4MA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.133 glucan 1,4-alpha-maltohydrolase

Temperature Stability

Temperature Stability on EC 3.2.1.133 - glucan 1,4-alpha-maltohydrolase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-20 - 40
the enzyme retains more than 50% of its activity at 40°C and 30°C for 10 min. The half-lives of the enzyme are 239.02 h, 126.03 h and 108.30 h at -20°C, 4°C and 20°C, respectively. However, the half-lives are dramatically reduced to 0.37 h and 0.04 h at 30°C and 40°C, respectively
105
melting temperature at pH 6.0
109
109°C melting temperature with enzymatic activity under acidic conditions (pH 3.5-5.0)
20 - 70
-
the enzyme shows 80% of the initial activity after an incubation time of 30 min without substrate in the temperature range of 20-70°C
210
-
baking at 210°C for 40 min leaves the enzyme active
40 - 70
-
the enzyme is stable up to 40°C at atmospheric pressure, but the activity of the maltogenic alpha-amylase is drastically reduced above 40°C. Hhigher temperatures (60 and 70°C) are destructive to the enzyme even in the presence of substrate starch
50 - 55
55
half-life at 55°C is about 15 min
55 - 75
the enzyme remains stable for up to 60 min at 55°C, after 60 min at 60 and 65°C the enzyme shows 75% activity, while after 20 min at 70 and 75°C the enzyme is inactive
60 - 70
-
stable at pH 5.5 at 60°C, 25% loss of activity at 70°C
64
-
Tm of Novamyl wild-type at pH 4.0
67
melting temperature at pH 4.0
77
-
Tm-value at pH 8 is 76.7°C
78
half-life of mutant III-1 about 20 times greater than half-life of the wild-type at 78°C
82
-
Tm-value of mutant R26Q/S169N/I333V/A398V/Q411L/P453L is 81.5°C
83
-
Tm of Novamyl wild-type at pH 5.0
85 - 100
-
purified enzyme is extremely thermostable with a half-life of 60 min at an optimal temperature of 95°C. The enzyme retains about 80% relative activity after 60 min of incubation at 85°C and 90°C, about 50% relative activity after 60 min of incubation at 95°C, and about 25% relative activity after 60 min of incubation at 100°C
88
-
TD of variant NM447 at pH 4.0, an increase of 10°C relative to the wild-type Novamyl
90
the half-life values of the monomeric enzyme at 80, 90 and 100°C are 35.7, 8.2 and 3.12 h, respectively. The half-life values of the dimeric enzyme at 80, 90 and 100°C are 55.7, 12.63 and 3.58 h, respectively
91
melting temperature at pH 5.0
95
-
Tm of variant NM447 at pH 5.0
additional information