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Literature summary for 3.2.1.133 extracted from

  • Kim, Y.W.; Choi, J.H.; Kim, J.W.; Park, C.; Cha, H.; Lee, S.B.; Oh, B.H.; Moon, T.W.; Park, K.H.
    Directed evolution of Thermus maltogenic amylase toward enhanced thermal resistance (2003), Appl. Environ. Microbiol., 69, 4866-4874.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.133

Protein Variants

Protein Variants Comment Organism
A398V random mutagenesis, using DNA shuffling Thermus sp.
I333V random mutagenesis, using DNA shuffling Thermus sp.
M375T random mutagenesis, using DNA shuffling Thermus sp.
P453L random mutagenesis, using DNA shuffling Thermus sp.
Q411L random mutagenesis, using DNA shuffling Thermus sp.
R26Q random mutagenesis, using DNA shuffling Thermus sp.
S169N random mutagenesis, using DNA shuffling Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, variant DM Thermus sp.
0.09
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, variant 4B74 Thermus sp.
0.16
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, wild-type and variants 3C71, 4B78 and 4A48 Thermus sp.
0.17
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, variants 1B76, 1B100 Thermus sp.
0.19
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, variant 2A39 Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
-
-
Thermus sp. IM6501
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Thermus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 pH 6.0, 60°C, beta-cyclodextrin as substrate, variant DM Thermus sp.
0.3
-
 pH 6.0, 60°C, beta-cyclodextrin as substrate, variant 4B74 Thermus sp.
1
-
 pH 6.0, 60°C, beta-cyclodextrin as substrate, variant 4B78 Thermus sp.
1.5
-
 pH 6.0, 60°C, beta-cyclodextrin as substrate, wild-type Thermus sp.
1.7
-
 pH 6.0, 60°C, beta-cyclodextrin as substrate, variants 2A39, 4A48 and 3C71 Thermus sp.
2
-
 pH 6.0, 60°C, beta-cyclodextrin as substrate, variant 1B100 Thermus sp.
2.1
-
 pH 6.0, 60°C, beta-cyclodextrin as substrate, variant 1B76 Thermus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 wild-type Thermus sp.
75
-
 ThMA-DM, highly thermostable mutant enzyme Thermus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 ThMA-DM, highly thermostable mutant enzyme, half-life 172 min, wild type enzyme completely inactivated in less than 1 min Thermus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, variants DM and 4B74 Thermus sp.
110
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, variant 4B78 Thermus sp.
120
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, variant 3C71 Thermus sp.
130
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, variant 4A48 Thermus sp.
160
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, wild-type Thermus sp.
170
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, variant 2A39 Thermus sp.
190
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, variant 1B100 Thermus sp.
200
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, variant 1B76 Thermus sp.