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3.2.1.133: glucan 1,4-alpha-maltohydrolase

This is an abbreviated version!
For detailed information about glucan 1,4-alpha-maltohydrolase, go to the full flat file.

Word Map on EC 3.2.1.133

Reaction

(Glcalpha(1-4))n
+
H2O
=
(Glcalpha(1-4))n-2
 +
alpha-maltose

Synonyms

alpha-amylase, AmyB, BbmA, BSMA, BSTA, BTMA, CAZy-GH13, glucan 1,4-alpha-maltohydrolase, glucan-1,4-alpha-maltohydrolase, Gt-MamyIII, LGMA, MAase, MABS, MAG1, maltogenase L, maltogenic alpha-amylase, maltogenic amylase, maltose-forming alpha-amylase, MAmy, MAUS149, More, NM319, NM326, NM398, NM404, NM447, Novamyl, PSMA, Smar_0613, SMMA, TCMA, Thermus maltogenic amylase, ThMA, TK4MA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.133 glucan 1,4-alpha-maltohydrolase

Cloned

Cloned on EC 3.2.1.133 - glucan 1,4-alpha-maltohydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a recombinant plasmid containing the enzymic gene and a constitutive promoter AmyR2 is constructed, and then transformed into a GRAS microorganism Bacillus subtilis
amplified in Escherichia coli DH5alpha, heterogenous expression in Lactococcus lactis MG1363, P170 expression system for production of recombinant LGMA
-
cloned and expressed in Escherichia coli
-
cloned and expressed in Escherichia coli BL21 (DE3)
-
cloned and overexpressed in Escherichia coli
-
cloned and overproduced in Escherichia coli MC1061
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cloning and expression in Escherichia coli MC 1061 with p6xHTMX, transformants grown in Luria-Bertani medium with ampicillin at 37°C
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-CodonPlus(DE3)-RP cells
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli DH5alpha using expression vectors pBMS1-4, open reading frame of 1749 bp encoding a protein of 582 residues, low sequence similarity of 60% to maltogenic amylase of Thermus sp., four known conserved regions at position 241 to 246, 324 to 331, 355 to 360, and 418 to 423 identified
expressed in Escherichia coli MC1061
-
expressed in Escherichia coli MC1061 cells
expressed in Escherichia coli MC1061, His-tagged, recombinant protein
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expressed in Escherichia coli MC1061, His-tagged, wild-type and mutant protein
expressed in the periplasm of Escherichia coli BL21(DE3) cells
-
expressed the gene in Escherichia coli
expression in Escherichia coli
-
expression in Escherichia coli BL21 (DE3)
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expression in Escherichia coli BL21 (DE3)as a His-tagged fusion protein
expression in Escherichia coli strains BL21(DE3)
gene amyM cloned in Escherichia coli, transferred with plasmid pDN400 carrier to a Bacillus subtilis 168 host and expressed heterogenously
-
gene GcaTK4MA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression of His6-tagged TK4MA in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM101
heterogenous expression in Bacillus subtilis host strain ISW1214
Novamyl libraries are iniatially made in Escherichia coli, then shuttled and expressed in the screen host Bacillus subtilis. The variants NM319, NM326, NM398, NM404, and NM447 are grown in fermenters, and the expressed enzymes are purified and tested for anti-staling properties in standard bread pH 5.5-5.9 and/or bread at sourdough (pH 4.0-4.3)
-
shuffled libraries of Novamyl are produced in in Escherichia coli, shuttled to screening host B. subtilis strain A164 delta 5, plasmid pHP13amp
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wild-type and mutant ThMAs expressed in Escherichia coli
-