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Literature summary for 3.2.1.133 extracted from

  • Kim, T.J.; Nguyen, V.D.; Lee, H.S.; Kim, M.J.; Cho, H.Y.; Kim, Y.W.; Moon, T.W.; Park, C.S.; Kim, J.W.; Oh, B.H.; Lee, S.B.; Svensson, B.; Park, K.H.
    Modulation of the multisubstrate specificity of Thermus maltogenic amylase by truncation of the N-terminal domain and by a salt-induced shift of the monomer/dimer equilibrium (2001), Biochemistry, 40, 14182-14190.
    View publication on PubMed
Search for more literature for 3.2.1.133

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km 5.34 mg mL-1, starch as substrate, pH 6.0, 60°C, hydrolysis, truncated mutant ThMA-DELTA124 Thermus sp.
additional information
-
 additional information Km 49.7 mg/ml, starch as substrate, pH 6.0, 60°C, wild-type, 1 M KCl Thermus sp.
additional information
-
 additional information Km 7.72 mg/ml, starch as substrate, pH 6.0, 60°C, truncated mutant ThMA-DELTA124, 1M KCl Thermus sp.
additional information
-
 additional information Km 61.0 mg/ml, starch as substrate, pH 6.0, 60°C, hydrolysis, wild-type Thermus sp.
0.263
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, wild-type Thermus sp.
0.308
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, wild-type, 1 M KCl Thermus sp.
0.36
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, truncated mutant ThMA DELTA124, 1M KCl Thermus sp.
0.461
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, truncated mutant ThMA- DELTA124 Thermus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 gel filtration Thermus sp.
94000 112000 gel filtration Thermus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Thermus sp.
-
 alpha-maltose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
truncated mutant ThMA-DELTA124 Thermus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4
-
 beta-cyclodextrin as substrate, dimer Thermus sp.
14.2
-
 starch as substrate Thermus sp.
21.1
-
 starch as substrate, 0.2 M KCl Thermus sp.
24
-
 starch as substrate, 0.4 M KCl Thermus sp.
26
-
 starch as substrate, 0.6 M KCl Thermus sp.
26.3
-
 starch as substrate, 0.8 M KCl Thermus sp.
27.6
-
 starch as substrate, 1.0 M KCl Thermus sp.
30.5
-
 soluble starch as substrate, monomer Thermus sp.
194.1
-
 beta-cyclodextrin as substrate, monomer Thermus sp.
206.8
-
 beta-cyclodextrin as substrate, 1.0 M KCl Thermus sp.
210
-
 beta-cyclodextrin as substrate, 0.8 M KCl Thermus sp.
227.2
-
 beta-cyclodextrin as substrate, 0.6 M KCl Thermus sp.
242.9
-
 beta-cyclodextrin as substrate, 0.4 M KCl Thermus sp.
257.8
-
 beta-cyclodextrin as substrate, 0.2 M KCl Thermus sp.
278
-
 beta-cyclodextrin as substrate Thermus sp.
342.7
-
 beta-cyclodextrin as substrate, dimer Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cyclodextrin + H2O
-
 Thermus sp. alpha-maltose + alpha-D-glucose
-
 ?
starch + H2O
-
 Thermus sp. alpha-maltose + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 68000, monomer/dimer equilibrium, ultracentrifugation Thermus sp.
homodimer
-
 Thermus sp.
monomer truncated mutant ThMAdelta124 exists only as a monomeric form Thermus sp.
monomer 1 * 68000, monomer/dimer equilibrium, ratio 48:52, ultracentrifugation Thermus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 truncated mutant ThMA-DELTA124 Thermus sp.
60
-
-
 Thermus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 truncated mutant ThMA-DELTA124 unstable Thermus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00000343
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, truncated mutant ThMA-DELTA124 Thermus sp.
0.00000367
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, truncated mutant ThMA- DELTA124, 1 M KCl Thermus sp.
0.000245
-
 starch pH 6.0, 60°C, hydrolysis, truncated mutant ThMA- DELTA124 Thermus sp.
0.000332
-
 starch pH 6.0, 60°C, hydrolysis, truncated mutant ThMA- DELTA124, 1 M KCl Thermus sp.
126
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, wild-type, 1 M KCl Thermus sp.
167
-
 beta-cyclodextrin pH 6.0, 60°C, hydrolysis, wild-type Thermus sp.
301
-
 starch pH 6.0, 60°C, hydrolysis, wild-type Thermus sp.
457
-
 starch pH 6.0, 60°C, hydrolysis, wild-type, 1 M KCl Thermus sp.