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Literature summary for 3.2.1.133 extracted from

  • Park, S.; Cha, H.; Kang, H.; Shim, J.; Woo, E.; Kim, J.; Park, K.
    Mutagenesis of Ala290, which modulates substrate subsite affinity at the catalytic interface of dimeric ThMA (2005), Biochim. Biophys. Acta, 1751, 170-177.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A290I mutant enzyme A290I produces mostly maltose from maltotetraose, while wild-type enzyme produces glucose as well as maltose. kcat/KM of mutant enzyme 290I for maltose is 48times less than that of wild-type enzyme. kcat/Km for maltotriose is 18.5fold lower than wild-type enzyme. kcat/Km for maltotetraose is 2.9fold lower than wild-type enzyme. kcat/Km for maltopentaose is 2.5fold lower than wild-type enzyme. kcat/Km for maltohexaose is 1.9fold lower than wild-type enzyme. kcat/Km for maltoheptaose is 4.7fold lower than wild-type enzyme Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13
-
maltotriose 50°C, pH 6.0, wild-type enzyme Thermus sp.
2.52
-
maltotriose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
2.95
-
maltotetraose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.38
-
maltotetraose 50°C, pH 6.0, wild-type enzyme Thermus sp.
3.5
-
maltopentaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
3.97
-
maltopentaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
7.16
-
maltohexaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
7.53
-
maltohexaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
8.18
-
maltoheptaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
9.21
-
maltoheptaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
63.43
-
maltose 50°C, pH 6.0, wild-type enzyme Thermus sp.
427
-
maltose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
recombinant
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzyme A290I Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + H2O
-
Thermus sp. maltose + D-glucose + ? mutant enzyme A290I produces mostly maltose, while wild-type enzyme produces glucose (32.8%) as well as maltose ?
maltohexaose + H2O
-
Thermus sp. ?
-
?
maltopentaose + H2O
-
Thermus sp. ?
-
?
maltose + H2O
-
Thermus sp. 2 D-glucose
-
?
maltotetraose + H2O
-
Thermus sp. maltose + 2 D-glucose mutant enzyme A290I produces mostly maltose, while wild-type enzyme produces glucose (24.8%) as well as maltose ?
maltotriose + H2O
-
Thermus sp. ?
-
?

Synonyms

Synonyms Comment Organism
ThMA
-
Thermus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.037 0.23 maltotriose 50°C, pH 6.0, mutant enzyme A29I Thermus sp.
0.04
-
maltose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
0.29
-
maltose 50°C, pH 6.0, wild-type enzyme Thermus sp.
2
-
maltoheptaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
9.2
-
maltohexaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
20 50 maltohexaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
29.8
-
maltotetraose 50°C, pH 6.0, wild-type enzyme Thermus sp.
42.5
-
maltoheptaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
69.01
-
maltopentaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
72.08
-
maltoheptaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
79.07
-
maltotriose 50°C, pH 6.0, mutant enzyme A29I Thermus sp.
88.45
-
maltohexaose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
160.3
-
maltohexaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
173.5
-
maltotetraose 50°C, pH 6.0, mutant enzyme A290I Thermus sp.
197
-
maltopentaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
384.1
-
maltoheptaose 50°C, pH 6.0, wild-type enzyme Thermus sp.
574.1
-
maltotetraose 50°C, pH 6.0, wild-type enzyme Thermus sp.
652.5
-
maltotriose 50°C, pH 6.0, wild-type enzyme Thermus sp.