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3.2.1.133: glucan 1,4-alpha-maltohydrolase

This is an abbreviated version!
For detailed information about glucan 1,4-alpha-maltohydrolase, go to the full flat file.

Word Map on EC 3.2.1.133

Reaction

(Glcalpha(1-4))n
+
H2O
=
(Glcalpha(1-4))n-2
 +
alpha-maltose

Synonyms

alpha-amylase, AmyB, BbmA, BSMA, BSTA, BTMA, CAZy-GH13, glucan 1,4-alpha-maltohydrolase, glucan-1,4-alpha-maltohydrolase, Gt-MamyIII, LGMA, MAase, MABS, MAG1, maltogenase L, maltogenic alpha-amylase, maltogenic amylase, maltose-forming alpha-amylase, MAmy, MAUS149, More, NM319, NM326, NM398, NM404, NM447, Novamyl, PSMA, Smar_0613, SMMA, TCMA, Thermus maltogenic amylase, ThMA, TK4MA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.133 glucan 1,4-alpha-maltohydrolase

Temperature Range

Temperature Range on EC 3.2.1.133 - glucan 1,4-alpha-maltohydrolase

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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 60
kinetics shown, more than 50% of activity retained at the temperature range of 10–50°C, rapid decrease in activity above 60°C
20 - 55
more than 50% activity between 20 and 50°C, at 55°C the enzyme retains 38% of activity
20 - 80
-
about 20% of activity maximum at 30°C, about 60% of activity maximum at 80°C
30 - 60
-
30°C: about 20% of maximal activity with starch, about 25% of maximal activity with beta-cyclodextrin and about 35% of maximal activity with pullulan, 60°C: about 40% of maximal activity with pullulan, about 50% of maximal activity with starch anf about 80% of maximal activity with beta-cyclodextrin
30 - 70
30°C: about 40% of maximal activity, 70°C: about 65% of maximal activity, hydrolysis of maltotriose, mutant enzyme W177Y
40 - 100
about 35% activity at 40°C, about 60% activity at 50°C, about 80% activity at 90°C, about 35% activity at 70°C, 100% activity at 80°C, about 97% activity at 90°C, and about 75% activity at 100°C
40 - 65
-
more than 50% activity between 40 and 65°C
40 - 70
about 40% activity at 40°C, about 80% activity at 50°C, 100% activity at 60°C, about 30% activity at 70°C
50 - 60
-
50°C: about 75% of maximal activity, 60°C: about 88% of maximal activity
50 - 80
50°C: about 70% of maximal activity, 80°C: about 40% of maximal activity, hydrolysis of maltotriose, wild-type enzyme
60 - 95
60°C; about 50% of maximal activity, 60°C: about 60% of maximal activity, alpha-1,4-glycosidic linkage hydrolysis of maltotriose
75 - 80
-
high thermostability, dependent on oligomer structure of the enzyme, optimal activity at 75°C and 80°C for beta-cyclodextrin and soluble starch, half-lifes of 61 min at 75°C and of 24 min at 80°C
80 - 105
-
at 105°C, almost 50% of the maximum activity is detected
80 - 98
activity at 80°C is about 50% compared to the activity at 98°C, alpha-1,6-glycosidic linkage hydrolysis of 6-O-maltotetraosyl-beta-cyclodextrin