Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | about 3.0% residual activity at 10 mM | Geobacillus sp. | |
Ba2+ | about 0.5% residual activity at 10 mM | Geobacillus sp. | |
Ca2+ | about 32% residual activity at 10 mM | Geobacillus sp. | |
Co2+ | about 1.1% residual activity at 10 mM | Geobacillus sp. | |
Cr3+ | about 22.2% residual activity at 10 mM | Geobacillus sp. | |
EDTA | complete inhibition at 10 mM | Geobacillus sp. | |
Fe2+ | about 0.4% residual activity at 10 mM | Geobacillus sp. | |
Hg2+ | about 2.7% residual activity at 10 mM | Geobacillus sp. | |
Li+ | about 41% residual activity at 10 mM | Geobacillus sp. | |
Mg2+ | about 1.4% residual activity at 10 mM | Geobacillus sp. | |
Mn2+ | about 1.5% residual activity at 10 mM | Geobacillus sp. | |
Na+ | about 1.7% residual activity at 10 mM | Geobacillus sp. | |
Ni2+ | about 0.7% residual activity at 10 mM | Geobacillus sp. | |
Sn4+ | about 89.3% residual activity at 10 mM | Geobacillus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 143.8% activity at 1 mM | Geobacillus sp. | |
K+ | about 186.6% activity at 1 mM | Geobacillus sp. | |
Li+ | about 166.5% activity at 1 mM | Geobacillus sp. | |
Sn4+ | about 121% activity at 1 mM | Geobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Geobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. | E0X988 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Geobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | high specificity for alpha-cyclodextrin | Geobacillus sp. | ? | - |
? | |
amylopectin + H2O | - |
Geobacillus sp. | ? | - |
? | |
amylose + H2O | - |
Geobacillus sp. | ? | - |
? | |
beta-cyclodextrin + H2O | - |
Geobacillus sp. | ? | - |
? | |
gamma-cyclodextrin + H2O | - |
Geobacillus sp. | ? | - |
? | |
glycogen + H2O | - |
Geobacillus sp. | ? | - |
? | |
starch + H2O | - |
Geobacillus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Geobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
MAase | - |
Geobacillus sp. |
maltogenic amylase | - |
Geobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 65 | - |
Geobacillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | about 40% activity at 40°C, about 80% activity at 50°C, 100% activity at 60°C, about 30% activity at 70°C | Geobacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 75 | the enzyme remains stable for up to 60 min at 55°C, after 60 min at 60 and 65°C the enzyme shows 75% activity, while after 20 min at 70 and 75°C the enzyme is inactive | Geobacillus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.38 | - |
amylopectin | in sodium-acetate buffer (50 mM, pH 6.0), at 60°C | Geobacillus sp. | |
62.82 | - |
amylose | in sodium-acetate buffer (50 mM, pH 6.0), at 60°C | Geobacillus sp. | |
225.6 | - |
gamma-cyclodextrin | in sodium-acetate buffer (50 mM, pH 6.0), at 60°C | Geobacillus sp. | |
280.8 | - |
beta-cyclodextrin | in sodium-acetate buffer (50 mM, pH 6.0), at 60°C | Geobacillus sp. | |
500 | - |
alpha-cyclodextrin | in sodium-acetate buffer (50 mM, pH 6.0), at 60°C | Geobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Geobacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | about 35% activity at pH 5.0, 100% activity at pH 6.0, about 85% activity at pH 7.0, about 60% activity at pH 8.0, about 40% activity at pH 9.0 | Geobacillus sp. |