Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | partial inactivation | Trigonella foenum-graecum | |
Mn2+ | strong inhibition | Trigonella foenum-graecum | |
Zn2+ | strong inhibition | Trigonella foenum-graecum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | increases the activity at low concentrations (1 and 2 mM) | Trigonella foenum-graecum | |
Fe2+ | increases the activity at low concentrations (1 and 2 mM) | Trigonella foenum-graecum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
x * 68000, SDS-PAGE | Trigonella foenum-graecum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Trigonella foenum-graecum | - |
maltose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trigonella foenum-graecum | - |
fenugreek | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and MonoQ Sepharose column chromatography | Trigonella foenum-graecum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Trigonella foenum-graecum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Trigonella foenum-graecum | maltose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, SDS-PAGE | Trigonella foenum-graecum |
Synonyms | Comment | Organism |
---|---|---|
maltogenic amylase | - |
Trigonella foenum-graecum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Trigonella foenum-graecum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | more than 50% activity between 40 and 65°C | Trigonella foenum-graecum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme is rapidly inactivated at temperature above 60°C and is totally inactivated at 60°C within 60 min in the absence of the substrate. However, in the presence of 1% (w/v) of soluble starch, the half time rises from approximately 10 to 60 min at 60°C | Trigonella foenum-graecum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Trigonella foenum-graecum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | more than 70% activity between pH 4.0 and 7.0, about 40% activity at pH 8.0 | Trigonella foenum-graecum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | the enzyme retains 100% of its activity when incubated for 24 h in a pH range between 5.0 and 10.0 | Trigonella foenum-graecum |