Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.1.26.11: tRNase Z

This is an abbreviated version!
For detailed information about tRNase Z, go to the full flat file.

Word Map on EC 3.1.26.11

Reaction

endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule =

Synonyms

(tRNase) Z, 3 tRNase, 3' tRNase, 3'-tRNA processing endoribonuclease, 3'-tRNase, AthTrz1, AthTrz2, AthTrz3, AthTrz4, BsuTrz, CelTrz, DmeTrz, EcoTrz, EcoZ, Elac, Elac1, ELAC2, HsaTrz1, HsaTrz2, HvoTrz, long form of 3' tRNase, long form of tRNA 3' processing endoribonuclease, long form of tRNase Z, MjaTrz, nuclease, transfer ribonucleate maturation 3'-endoribo-(9CI), PaeTrz, pre-tRNA processing endoribonuclease, precursor tRNA 3'-end processing endoribonuclease, ribonuclease Z, RNase BN, RNase Z, RNase ZL, RNase ZS1, RNaseZ, RNZ, SceTrz, SpTrz1p, SpTrz2p, TM0207, TmaTrz, TMS5 protein, transfer RNA maturation endonuclease, tRNA 3 endonuclease, tRNA 3' processing endoribonuclease, tRNA 3'-processing enzyme tRNase Z, tRNA precursor-processing endoribonuclease, tRNase Z, tRNase Z2, tRNase ZL, tRNase ZS, tRNaseZ, tRnaseZL, tRnaseZS, Trz, TRZ1, Trz1p, TRZ2, TrZL1, TrZL2, TrZS1, TrZS2, YP_145327, YqiK, YqjK, zinc phosphodiesterase, zinc-dependent phosphodiesterase, ZiPD

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.11 tRNase Z

Purification

Purification on EC 3.1.26.11 - tRNase Z

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography, gel filtration
-
by centrifugation, on nickel column and by ultrafiltration
-
chromatography steps
-
full-length and DELTA30 tRNase ZL recombinant proteins purified on nickel column or on chitin beads
-
gel filtration
-
His-tag fusion, Ni-affinity chromatography
His-tag, Ni-affinity chromatography
-
more than 90% pure according to SDS-PAGE
Ni-NTA agarose column chromatography
-
Ni2+-NTA column chromatography
-
nickel affinity chromatography
-
nickel chelate affinity purification
nickel chelate affinity purification of soluble tRNase Z
nickel-chelate resin column chromatography and Superose 6 gel filtration
prior to crystallization
-
recombinant TrZS1 purified. TrZL2 protein purified using the S tag
TrZL1 protein purified using the S tag
TrZS2 protein purified using the S tag