Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.11 extracted from

  • Canino, G.; Bocian, E.; Barbezier, N.; Echeverria, M.; Forner, J.; Binder, S.; Marchfelder, A.
    Arabidopsis encodes four tRNase Z enzymes (2009), Plant Physiol., 150, 1494-1502.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.11

Cloned(Commentary)

Cloned (Comment) Organism
TrZL1 cloned into pBluescript KS II, subcloned into the NotI/XhoI sites of pET29 and expressed in Escherichia coli BL21-AI cells Arabidopsis thaliana
TrZS1 expressed as described previously. TrZL2 cloned into pBluescript KS II, subcloned into the pET29 expression vector previously digested with BamHI/XhoI and expressed in Escherichia coli Rosetta cells Arabidopsis thaliana
TrZS2 cloned into the pUC18 vector, subcloned into the NcoI/XhoI sites of pET32 expression vector and expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells as a fusion protein with both His and S tags Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-
cytoplasm TrZS1 Arabidopsis thaliana 5737
-
mitochondrion TrZL2 Arabidopsis thaliana 5739
-
nucleus
-
 Arabidopsis thaliana 5634
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Q8L633
-
-
Arabidopsis thaliana Q8LGU7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant TrZS1 purified. TrZL2 protein purified using the S tag Arabidopsis thaliana
TrZL1 protein purified using the S tag Arabidopsis thaliana
TrZS2 protein purified using the S tag Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
additional information mRNAs corresponding to the tRNase Z proteins are found in all plant tissues. No significant variation between mRNA levels for TrZS1 and TrZL2 Arabidopsis thaliana
-
additional information mRNAs corresponding to the tRNase Z proteins are found in all plant tissues. No significant variation between mRNA levels for TrZS1, TrZS2, TrZL1 and TrZL2 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
precursor tRNATyr + H2O all four recombinant tRNase Z proteins have tRNA 3'-processing activity Arabidopsis thaliana tRNA + 3' trailer
-
 ?
precursor tRNATyr + H2O all four recombinant tRNase Z proteins have tRNA 3'-processing activity. TrZL2 processes the precursor less efficiently, which may be due to the fact that this enzyme is difficult to express and only low amounts of recombinant protein can be obtained Arabidopsis thaliana tRNA + 3' trailer
-
 ?

Synonyms

Synonyms Comment Organism
tRNase Z
-
 Arabidopsis thaliana
TrZL1
-
 Arabidopsis thaliana
TrZL2
-
 Arabidopsis thaliana
TrZS1
-
 Arabidopsis thaliana
TrZS2
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana all tRNase Z genes are expressed during all growth stages of Arabidopsis, in particular during the first stage of development (seedling) and at the start of flowering up

General Information

General Information Comment Organism
malfunction deletion of the chloroplastic TrZS2 gene results in an embryo-lethal phenotype Arabidopsis thaliana
malfunction The knockout TrZL1 mutant shows no visible phenotype Arabidopsis thaliana
malfunction The knockout TrZL2 and TrZS1 mutants show no visible phenotypes Arabidopsis thaliana
physiological function Arabidopsis encodes four tRNase Z, two short forms (TrZS1 and TrZS2) and two long forms (TrZL1 and TrZL2) Arabidopsis thaliana
physiological function Arabidopsis encodes four tRNase Z, two short forms (TrZS1 and TrZS2) and two long forms (TrZL1 and TrZL2). The mitochondrial tRNase Z protein TrZL2 cleaves tRNA-like elements that serve as processing signals in mitochondrial mRNA maturation Arabidopsis thaliana
physiological function Arabidopsis encodes four tRNase Z, two short forms (TrZS1 and TrZS2) and two long forms (TrZL1 and TrZL2). TrZL1 is the only tRNase Z responsible for the maturation of nuclear tRNAs. The mitochondrial tRNase Z proteins TrZL1 cleave tRNA-like elements that serve as processing signals in mitochondrial mRNA maturation Arabidopsis thaliana