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Literature summary for 3.1.26.11 extracted from

  • Spath, B.; Kirchner, S.; Vogel, A.; Schubert, S.; Meinlschmidt, P.; Aymanns, S.; Nezzar, J.; Marchfelder, A.
    Analysis of the functional modules of the tRNA 3' endonuclease (tRNase Z) (2005), J. Biol. Chem., 280, 35440-35447.
    View publication on PubMed
Search for more literature for 3.1.26.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3)pLys Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C25G dimerization, RNA binding, 33% activity compared to wild-type Arabidopsis thaliana
C40G dimerization, RNA binding, similar activity compared to wild-type Arabidopsis thaliana
D185G dimerization, RNA binding, 7% activity compared to wild-type Arabidopsis thaliana
D58A dimerization, RNA binding, no activity Arabidopsis thaliana
Deletion A252 dimerization, RNA binding, no activity Arabidopsis thaliana
Delta200-212 weak dimerization, no RNA binding, no activity Arabidopsis thaliana
Delta270-280 multimerization, no RNA binding, no activity Arabidopsis thaliana
Delta49-164 weak dimerization, no RNA binding, no activity Arabidopsis thaliana
Delta51-60 weak dimerization, no RNA binding, no activity Arabidopsis thaliana
E208A dimerization, RNA binding, 55% activity compared to wild-type Arabidopsis thaliana
F51L dimerization, RNA binding, similar activity compared to wild-type Arabidopsis thaliana
G184V weak dimerization, no RNA binding, no activity Arabidopsis thaliana
G62V dimerization, RNA binding, 26% activity compared to wild-type Arabidopsis thaliana
H133L dimerization, weak RNA binding, no activity Arabidopsis thaliana
H226L dimerization, RNA binding, no activity Arabidopsis thaliana
H248L multimerization, no RNA binding, no activity Arabidopsis thaliana
H54L dimerization, weak RNA binding, no activity Arabidopsis thaliana
H56L weak dimerization, weak RNA binding, no activity Arabidopsis thaliana
H59L multimerization, no RNA binding, no activity Arabidopsis thaliana
K203I multimerization, no RNA binding, no activity Arabidopsis thaliana
L205I weak dimerization, RNA binding, 56% activity compared to wild-type Arabidopsis thaliana
P178A dimerization, RNA binding, 74% activity compared to wild-type Arabidopsis thaliana
P64A dimerization, RNA binding, similar activity compared to wild-type Arabidopsis thaliana
P83L multimerization, no RNA binding, no activity Arabidopsis thaliana
R252G dimerization, RNA binding, 26% activity compared to wild-type Arabidopsis thaliana
T186I multimerization, no RNA binding, no activity Arabidopsis thaliana
T210I weak dimerization, RNA binding, 85% activity compared to wild-type Arabidopsis thaliana
Y140L weak dimerization, RNA binding, 30% activity compared to wild-type Arabidopsis thaliana
Y253S dimerization, RNA binding, 23% activity compared to wild-type Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8LGU7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNATyr + H2O
-
 Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
dimer cross-linking, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
tRNase Z
-
 Arabidopsis thaliana
TRZ1
-
 Arabidopsis thaliana