3.1.26.11: tRNase Z
This is an abbreviated version!
For detailed information about tRNase Z, go to the full flat file.
Reaction
endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule
=
Synonyms
(tRNase) Z, 3 tRNase, 3' tRNase, 3'-tRNA processing endoribonuclease, 3'-tRNase, AthTrz1, AthTrz2, AthTrz3, AthTrz4, BsuTrz, CelTrz, DmeTrz, EcoTrz, EcoZ, Elac, Elac1, ELAC2, HsaTrz1, HsaTrz2, HvoTrz, long form of 3' tRNase, long form of tRNA 3' processing endoribonuclease, long form of tRNase Z, MjaTrz, nuclease, transfer ribonucleate maturation 3'-endoribo-(9CI), PaeTrz, pre-tRNA processing endoribonuclease, precursor tRNA 3'-end processing endoribonuclease, ribonuclease Z, RNase BN, RNase Z, RNase ZL, RNase ZS1, RNaseZ, RNZ, SceTrz, SpTrz1p, SpTrz2p, TM0207, TmaTrz, TMS5 protein, transfer RNA maturation endonuclease, tRNA 3 endonuclease, tRNA 3' processing endoribonuclease, tRNA 3'-processing enzyme tRNase Z, tRNA precursor-processing endoribonuclease, tRNase Z, tRNase Z2, tRNase ZL, tRNase ZS, tRNaseZ, tRnaseZL, tRnaseZS, Trz, TRZ1, Trz1p, TRZ2, TrZL1, TrZL2, TrZS1, TrZS2, YP_145327, YqiK, YqjK, zinc phosphodiesterase, zinc-dependent phosphodiesterase, ZiPD
ECTree
Specific Activity
Specific Activity on EC 3.1.26.11 - tRNase Z
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
in vitro processing activities of different recombinant tRNase Z enzymes with respect to CCA-containing and CCA-less pre-tRNAs summarized
additional information
-
in vitro tRNA processing assays, bis(p-nitrophenyl)phosphate hydrolysis assay described, kinetic analysis shown, AthTRZ1 effective catalyst of bpNPP hydrolysis, AthTRZ1-R252G reveals ten times higher activity compared to the wild-type enzyme, in contrast to the short tRNase Z enzyme AthTRZ1, long tRNase Z enzymes do not have bis(p-nitrophenyl)phosphate hydrolysis activity
additional information
-
in vitro processing activities of different recombinant tRNase Z enzymes with respect to CCA-containing and CCA-less pre-tRNAs reviewed, involvement in potential futile cycles in tRNA 3'-processing shown
additional information
-
in vitro tRNA processing assays, bpNPP hydrolysis assay described, in contrast to the short tRNase Z enzyme AthTRZ1 of Arabidopsis thaliana, long tRNase Z enzymes do not have bis(p-nitrophenyl)phosphate hydrolysis activity
additional information
reaction kinetics of tRNase Z variants with Ala substitutions through conserved loops on the carboxy side of motif II shown, substitution of motif IV aspartate reduces catalytic efficiency more than 10000fold, Histidines in motif III, V, and the HST loop also functionally important, replacement of Glu in the HEAT loop with Ala reduces efficiency by about 1000fold
additional information
-
evidences for sigmoidal saturation kinetics discussed
additional information
-
in vitro processing activities of different recombinant tRNase Z enzymes with respect to CCA-containing and CCA-less pre-tRNAs reviewed
additional information
-
involvement in 3'-end processing of pre-mRNAs, additional roles in 3'-end processing of RNA precursors, DNA repair, V(D)J recombination, and telomere maintenance reviewed
additional information
-
involvement in 3'-end processing of pre-mRNAs, roles in 3'-end processing of RNA precursors, DNA repair, V(D)J recombination, and telomere maintenance reviewed, linkage to human disease given, overview
additional information
in vitro tRNA processing assays, bis(p-nitrophenyl)phosphate hydrolysis assay described, in contrast to the short tRNase Z enzyme AthTRZ1 of Arabidopsis thaliana, long tRNase Z enzymes do not have bis(p-nitrophenyl)phosphate hydrolysis activity
additional information
-
reaction kinetics for in vitro 3'-processing of pre-tRNAArg(74CCA76) and pre-tRNAArg(74GUG76) in the presence of 10mM Mg2+ or 0.2 mM Mn2+ shown