Literature summary for 3.1.26.11 extracted from

Elbarbary, R.A.; Takaku, H.; Uchiumi, N.; Tamiya, H.; Abe, M.; Takahashi, M.; Nishida, H.; Nashimoto, M.
Modulation of gene expression by human cytosolic tRNase Z(L) through 5-half-tRNA (2009), PLoS ONE, 4, e5908.

Search for more literature for 3.1.26.11

Cloned(Commentary)

Cloned (Comment)	Organism
histidine-tagged full-length and DELTA30 tRNase ZL proteins overexpressed from the expression plasmid pQE-80L in Escherichia coli strain Rosetta(DE3)pLysS. Full-length and DELTA30 tRNase ZL proteins also overexpressed from the expression plasmid pTYB11. 293 cells cotransfected with one of the pGL3 series plasmids and the expression plasmid pTK-b together with chemically synthesized 5'-half-tRNAGlu, the 28S rRNA fragment, the tRNase ZL or tRNase ZS expression plasmid, the tRNase ZL siRNA, or the four human Ago2 siRNAs	Homo sapiens

Cloned (Comment)

Organism

histidine-tagged full-length and DELTA30 tRNase ZL proteins overexpressed from the expression plasmid pQE-80L in Escherichia coli strain Rosetta(DE3)pLysS. Full-length and DELTA30 tRNase ZL proteins also overexpressed from the expression plasmid pTYB11. 293 cells cotransfected with one of the pGL3 series plasmids and the expression plasmid pTK-b together with chemically synthesized 5'-half-tRNAGlu, the 28S rRNA fragment, the tRNase ZL or tRNase ZS expression plasmid, the tRNase ZL siRNA, or the four human Ago2 siRNAs

Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	DELTA30 tRNase ZL exists primarily in the cytosol	Homo sapiens	5829	-
nucleus	full-length form is primarily present in the nuclear fraction	Homo sapiens	5634	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
94000	-	DELTA30 tRNase ZL, Western blotting	Homo sapiens
98000	-	full-length tRNase ZL, Western blotting	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
full-length and DELTA30 tRNase ZL recombinant proteins purified on nickel column or on chitin beads	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
A-549 cell	-	Homo sapiens	-
Hep-G2 cell	-	Homo sapiens	-
IMR-90 cell	-	Homo sapiens	-
additional information	293 cell	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	PPM1F and DYNC1H1 mRNAs are genuine targets of tRNase ZL guided by 5'-half-tRNAGlu and the 28S rRNA fragment, respectively	Homo sapiens	?	-	?
pre-tRNAarg + H2O	cleavage by full-length tRNase ZL and DELTA30 tRNase ZL with the same efficiency	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
long form of 3' tRNase	-	Homo sapiens
long form of tRNA 3' processing endoribonuclease	-	Homo sapiens
long form of tRNase Z	-	Homo sapiens
tRNase ZL	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	siRNA targeting the tRNase ZL mRNA	down

General Information

General Information	Comment	Organism
physiological function	cytosolic tRNase ZL modulates gene expression through 5'-half-tRNA. The tRNase ZL is likely to be involved in the p53 signaling pathway and apoptosis	Homo sapiens