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Literature summary for 3.1.26.11 extracted from

  • Yan, H.; Zareen, N.; Levinger, L.
    Naturally occurring mutations in human mitochondrial pre-tRNASer(UCN) can affect the transfer ribonuclease Z cleavage site, processing kinetics, and substrate secondary structure (2006), J. Biol. Chem., 281, 3926-3935.
    View publication on PubMed
Search for more literature for 3.1.26.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf-9 cells Homo sapiens
expression in Escherichia coli BL21(DE3) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000004
-
 pre-tRNASer(UCN)7512G 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.0000048
-
 pre-tRNASer(UCN) 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.0000069
-
 pre-tRNASer(UCN)7444U 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.0000076
-
 pre-tRNASer(UCN)7511G 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.0000108
-
 pre-tRNASer(UCN)7510G 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.0000197
-
 pre-tRNASer(UCN)7445C 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.000022
-
 pre-tRNAArg 25 mM K-MOPS, pH 6.75, 2 mM MgCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.0000633
-
 pre-tRNASer(UCN)7443C 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.0000855
-
 pre-tRNASer(UCN)7445G 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.000132
-
 pre-tRNAArg 25 mM K-MOPS, pH 6.75, 1 mM MnCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Homo sapiens
86000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tag, Ni-affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNAarg + H2O
-
 Homo sapiens ?
-
 ?
pre-tRNASer(UCN) + H2O wild type Homo sapiens ?
-
 ?
pre-tRNASer(UCN)7443C + H2O natural tRNA mutant Homo sapiens ?
-
 ?
pre-tRNASer(UCN)7444U + H2O natural tRNA mutant Homo sapiens ?
-
 ?
pre-tRNASer(UCN)7445C + H2O natural tRNA mutant Homo sapiens ?
-
 ?
pre-tRNASer(UCN)7445G + H2O natural tRNA mutant Homo sapiens ?
-
 ?
pre-tRNASer(UCN)7510G + H2O natural tRNA mutant Homo sapiens ?
-
 ?
pre-tRNASer(UCN)7511G + H2O natural tRNA mutant Homo sapiens ?
-
 ?
pre-tRNASer(UCN)7512G + H2O natural tRNA mutant Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
(tRNase) Z
-
 Homo sapiens
RNase Z
-
 Homo sapiens
tRNase ZL
-
 Homo sapiens
tRNase ZS
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 pre-tRNASer(UCN)7445C 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.021
-
 pre-tRNAArg 25 mM K-MOPS, pH 6.75, 1 mM MnCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.05
-
 pre-tRNASer(UCN)7512G 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.05
-
 pre-tRNASer(UCN)7511G 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.05
-
 pre-tRNASer(UCN)7510G 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.07
-
 pre-tRNASer(UCN) 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.153
-
 pre-tRNASer(UCN)7444U 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.23
-
 pre-tRNASer(UCN)7443C 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
0.345
-
 pre-tRNASer(UCN)7445G 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens
6.18
-
 pre-tRNAArg 25 mM K-MOPS, pH 6.75, 2 mM MgCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin Homo sapiens