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415-nt CAT mRNA + H2O
?
-
5'-half portion of the E. coli chloramphenicol acetyltransferase mRNA
-
-
?
Bacillus subtilis pre-tRNATrp + H2O
?
Bacillus subtilis tRNACys + H2O
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
Escherichia coli pre-tRNAPhe + H2O
?
GT7H + H2O
?
-
complex of SPH2 with 5' half tRNA
-
-
?
GT7HM10 + H2O
?
-
complex of SPH2 with 5' half tRNA
-
-
?
GT7HM20 + H2O
?
-
complex of SPH2 with 5' half tRNA
-
-
?
GT7HM22 + H2O
?
-
complex of SPH2 with 5' half tRNA
-
-
?
human pre-tRNAArg + H2O
?
mt-pre-tRNAArg + H2O
mt-tRNAArg + 3'-leader of tRNA
-
-
-
-
?
mt-pre-tRNAGlu + H2O
mt-tRNAGlu + 3'-leader of tRNA
-
-
-
-
?
mt-pre-tRNAGly + H2O
mt-tRNAGly + 3'-leader of tRNA
-
-
-
-
?
mt-pre-tRNALeu + H2O
mt-tRNALeu + 3'-leader of tRNA
-
-
-
-
?
mt-pre-tRNALys + H2O
mt-tRNALys + 3'-leader of tRNA
-
-
-
-
?
mt-pre-tRNAVal + H2O
mt-tRNAVal + 3'-leader of tRNA
-
-
-
-
?
pre-tRNA(Arg) + H2O
?
-
-
-
-
?
pre-tRNA(Val) + H2O
?
-
-
-
-
?
pre-tRNA-CAG-trailer + H2O
?
-
5'-, 3'-extended chloroplast pre-tRNAPhe
-
-
?
pre-tRNA-CCA-trailer + H2O
?
pre-tRNA-CCAOH-trailer + H2O
?
pre-tRNA9-AspATC + H2O
?
-
-
-
-
?
pre-tRNAArg + H2O
tRNAArg + 3'-leader of tRNA
-
-
-
-
?
pre-tRNAArg(74CCA76) + H2O
?
-
reaction kinetics for in vitro 3'-processing using engineered enzyme variants, in presence of 10 mM Mg2+ or of 0.2 mM Mn2+
-
-
?
pre-tRNAArg(74GUG76) + H2O
?
-
cleavage of pre-tRNAArg(74GUG76) by engineered variants about 7 to more than 16fold less efficient than that of pre-tRNAArg(74CCA76)
-
-
?
pre-tRNAAsp + H2O
?
-
the enzyme catalyzes endonucleolytic tRNA 3' processing
-
-
?
pre-tRNAGlu-CCAN17 + H2O
?
-
-
-
-
?
pre-tRNAGlu-CCUN17 + H2O
?
-
-
-
-
?
pre-tRNAGlu-CUAN17 + H2O
?
-
-
-
-
?
pre-tRNAGlu-UCAN17 + H2O
?
-
-
-
-
?
pre-tRNAGlu-UUAN17 + H2O
?
-
-
-
-
?
pre-tRNAGlu-UUUN17 + H2O
?
-
-
-
-
?
pre-tRNAGly + H2O
?
-
-
-
-
?
pre-tRNAHis-AUG + H2O
?
-
-
-
?
pre-tRNAHis-CCA + H2O
?
-
-
-
?
pre-tRNALeu + H2O
?
-
the enzyme catalyzes endonucleolytic tRNA 3' processing
-
-
?
pre-tRNALeu(CUN) + H2O
?
-
-
-
-
?
pre-tRNALeu(UUR) + H2O
?
-
-
-
-
?
pre-tRNAPhe + H2O
?
-
the enzyme catalyzes endonucleolytic tRNA 3' processing
-
-
?
pre-tRNASer + H2O
?
-
the enzyme catalyzes endonucleolytic tRNA 3' processing
-
-
?
pre-tRNASer(AGY) + H2O
?
-
-
-
-
?
pre-tRNASer(UCN) + H2O
?
-
wild type
-
-
?
pre-tRNASer(UCN)7443C + H2O
?
-
natural tRNA mutant
-
-
?
pre-tRNASer(UCN)7444U + H2O
?
-
natural tRNA mutant
-
-
?
pre-tRNASer(UCN)7445C + H2O
?
-
natural tRNA mutant
-
-
?
pre-tRNASer(UCN)7445G + H2O
?
-
natural tRNA mutant
-
-
?
pre-tRNASer(UCN)7510G + H2O
?
-
natural tRNA mutant
-
-
?
pre-tRNASer(UCN)7511G + H2O
?
-
natural tRNA mutant
-
-
?
pre-tRNASer(UCN)7512G + H2O
?
-
natural tRNA mutant
-
-
?
pre-tRNAThr + H2O
?
-
-
-
-
?
precursor tRNASer(UGA)-M + H2O
mature tRNASer(UGA)-M
precursor tRNASer(UGA)-M + H2O
mature tRNASer(UGA)-M + ?
-
overexpression of untagged TRZ1, but not FLAG-tagged TRZ1, increases the abundance of mature tRNASer(UGA)-M in strain yYH1
-
-
?
precursor tRNATyr + H2O
tRNA + 3' trailer
pretRNA35-MetCAT + H2O
?
-
-
-
-
?
pTyrI + H2O
?
-
pre-tRNATyr from Oenothera berteriana
-
-
?
R-A1 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-ACA19 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-ACA3 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-ASA1 + H2O
?
-
pre-tRNAArg construct with one base-pair addition after the fifth base-pair of the acceptor stem
-
-
?
R-ASA2 + H2O
?
-
pre-tRNAArg construct with two base-pair additions after the fifth base-pair of the acceptor stem
-
-
?
R-ASA3 + H2O
?
-
pre-tRNAArg construct with three base-pair additions after the fifth base-pair of the acceptor stem
-
-
?
R-ASD1 + H2O
?
-
pre-tRNAArg construct with one base-pair deletion after the third base-pair of the acceptor stem
-
-
?
R-ASD2 + H2O
?
-
pre-tRNAArg construct with two base-pair deletions after the third base-pair of the acceptor stem
-
-
?
R-AT12A + H2O
?
-
pre-tRNAArg construct with one additional base-pair in the acceptor stem and one deleted base-pair in the T stem
-
-
?
R-AT12B + H2O
?
-
pre-tRNAArg construct with one deleted base-pair in the acceptor stem and one additional base-pair in the T stem
-
-
?
R-AT14 + H2O
?
-
pre-tRNAArg construct with one base-pair addition in both acceptor and T stems
-
-
?
R-AT16 + H2O
?
-
pre-tRNAArg construct with two base-pair additions in both acceptor and T stems
-
-
?
R-ATM1 + H2O
?
-
small pre-tRNAArg construct
-
-
?
R-ATM10 + H2O
?
-
small pre-tRNAArg construct
-
-
?
R-ATM11 + H2O
?
-
small pre-tRNAArg construct
-
-
?
R-ATM2 + H2O
?
-
small pre-tRNAArg construct
-
-
?
R-ATM3 + H2O
?
-
small pre-tRNAArg construct
-
-
?
R-ATM4 + H2O
?
-
small pre-tRNAArg construct
-
-
?
R-ATM5 + H2O
?
-
small pre-tRNAArg construct
-
-
?
R-ATM7 + H2O
?
-
small pre-tRNAArg construct
-
-
?
R-ATM8 + H2O
?
-
small pre-tRNAArg construct
-
-
?
R-ATM9 + H2O
?
-
small pre-tRNAArg construct
-
-
?
R-ATW + H2O
?
-
small pre-tRNAArg
-
-
?
R-CCA6 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-CCA8 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-CCG + H2O
?
-
pre-tRNAArg construct
-
-
?
R-CUA + H2O
?
-
pre-tRNAArg construct
-
-
?
R-G10 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-G13 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-G15 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-G19 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-G8 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-GCA + H2O
?
-
pre-tRNAArg construct
-
-
?
R-L0 + H2o
?
-
wild type pre-tRNAArg
-
-
?
R-L3 + H2o
?
-
pre-tRNAArg construct
-
-
?
R-L6 + H2o
?
-
pre-tRNAArg construct
-
-
?
R-TASD2 + H2O
?
-
-
-
-
?
R-TSA1 + H2O
?
-
pre-tRNAArg construct with one base-pair addition in the T stem
-
-
?
R-TSA2 + H2O
?
-
pre-tRNAArg construct with two base-pair additions in the T stem
-
-
?
R-TSD1 + H2O
?
-
pre-tRNAArg construct with one base-pair deletion in the T stem
-
-
?
R-TSD2 + H2O
?
-
pre-tRNAArg construct with two base-pair deletions in the T stem
-
-
?
R-U1 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-UCA + H2O
?
-
pre-tRNAArg construct
-
-
?
R-UUU + H2O
?
-
small pre-tRNAArg construct
-
-
?
S-D-lactoylglutathione + H2O
?
-
is a glyoxalase II substrate, tRNase Z has a broad substrate spectrum, is able to process a substrate belonging to a different subclass of the MBL family
-
-
?
T7-5 + H2O
?
-
complex of SPH2 with shorter RNA
-
-
?
T7M7 + H2O
?
-
complex of SPH2 with RNA heptamer
-
-
?
T7M71 + H2O
?
-
complex of SPH2 with RNA heptamer
-
-
?
Thermoplasma acidophilum pre-tRNAPhe + H2O
?
weak cleavage
-
-
?
Thermotoga maritima pre-tRNAArg(CCA) + H2O
?
Thermotoga maritima pre-tRNAArg(GUG) + H2O
?
Thermotoga maritima pre-tRNAMet(CCA) + H2O
?
Thermotoga maritima pre-tRNAMet(UAG) + H2O
?
thymidine 5'-p-nitrophenyl phosphate + H2O
p-nitrophenol + TMP
-
-
-
-
?
thymidine-5-p-nitrophenyl phosphate + H2O
p-nitrophenol + TMP
-
TpNPP substrate, phosphodiesterase activities of tRNase Z on small chromogenic substrates mentioned, structural features of potential model substrates indicated
-
-
?
tRNAHis48 3'+ C + H2O
?
-
-
-
-
?
tRNAHis48 3'+ CC + H2O
?
-
-
-
-
?
tRNATyr + H2O
?
-
wild type tRNA precursor
-
-
?
trnB-THr + H2O
?
-
pre-tRNATHr with a 47 nucleotide 3' trailing sequence and CCA motif, no cleavage
-
-
?
trnI-Thr + H2O
?
-
pre-tRNAThr with a 83 nucleotide 3' trailing sequence, cleavage at one or two bases downstream the discriminator base
-
-
?
trnI-Thr-CAAATG-trailer + H2O
?
-
-
-
-
?
trnI-Thr-CCAATG-trailer + H2O
?
-
-
-
-
?
trnI-Thr-TAAATG-trailer + H2O
?
-
native trailer sequence
-
-
?
trnI-Thr-TCAATG-trailer + H2O
?
-
-
-
-
?
ubiquitin fusion ribosomal protein L40 mRNA + H2O
?
-
-
-
-
?
ubiquitin fusion ribosomal protein L401 mRNA + H2O
?
-
-
-
-
?
ubiquitin fusion ribosomal protein L402 mRNA + H2O
?
-
-
-
-
?
ubiquitin fusion ribosomal protein L403 mRNA + H2O
?
-
-
-
-
?
additional information
?
-
Bacillus subtilis pre-tRNATrp + H2O
?
cleaved well after C75 relatively
-
-
?
Bacillus subtilis pre-tRNATrp + H2O
?
cleaved well after C75 relatively
-
-
?
Bacillus subtilis tRNACys + H2O
?
efficient cleavage
-
-
?
Bacillus subtilis tRNACys + H2O
?
efficient cleavage
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
differentiation of tRNase Z variants by 3'-processing activity and their ability to hydrolyze the phosphodiester bond in the chromogenic phosphodiester bis(p-nitrophenyl)phosphate (bpNPP), smallest known tRNase Z substrate, fourteen variants lost ability to hydrolyze bpNPP, seven variants reveal reduced activity
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
differentiation of tRNase Z variants by the substrate bis(p-nitrophenyl)phosphate (bpNPP) described, smallest known tRNase Z substrate
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
no hydrolysis of the the chromogenic phosphodiester bis(p-nitrophenyl)phosphate (bpNPP) observed
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
bpNPP substrate, phosphodiesterase activities of tRNase Z on small chromogenic substrates mentioned, structural features of potential model substrates indicated
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
evidences for sigmoidal saturation kinetics with the small chromogenic phosphodiester substrate reviewed
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
displays efficient phosphodiesterase activity against bis(p-nitrophenyl) phosphate, which is unusual among the RNase Z family of enzymes
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
phosphodiesterase activity
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
-
-
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
no hydrolysis of the the chromogenic phosphodiester bis(p-nitrophenyl)phosphate (bpNPP) observed
-
-
?
Escherichia coli pre-tRNAPhe + H2O
?
cleavage is barely detected
-
-
?
Escherichia coli pre-tRNAPhe + H2O
?
cleavage is barely detected
-
-
?
GENV7-Env + H2O
?
-
complex of SPH2 with 5' half tRNAArg
-
-
?
GENV7-Env + H2O
?
-
complex of SPH2 with 5' half tRNAArg
-
-
?
human pre-tRNAArg + H2O
?
efficient cleavage
-
-
?
human pre-tRNAArg + H2O
?
efficient cleavage
-
-
?
human pre-tRNAArg + H2O
?
efficient cleavage
-
-
?
IM0 + H2O
?
-
pre-tRNAIle with CCA 3'-trailer sequence
-
-
?
IM0 + H2O
?
-
pre-tRNAIle with CCA 3'-trailer sequence
-
-
?
IM0 + H2O
?
-
pre-tRNAIle with CCA 3'-trailer sequence
-
-
?
IM1 + H2O
?
-
pre-tRNAIle with CCG 3'-trailer sequence
-
-
?
IM1 + H2O
?
-
pre-tRNAIle with CCG 3'-trailer sequence
-
-
?
IM1 + H2O
?
-
pre-tRNAIle with CCG 3'-trailer sequence
-
-
?
IM2 + H2O
?
-
pre-tRNAIle with CUG 3'-trailer sequence
-
-
?
IM2 + H2O
?
-
pre-tRNAIle with CUG 3'-trailer sequence
-
-
?
IM2 + H2O
?
-
pre-tRNAIle with CUG 3'-trailer sequence
-
-
?
IM3 + H2O
?
-
pre-tRNAIle with UUG 3'-trailer sequence
-
-
?
IM3 + H2O
?
-
pre-tRNAIle with UUG 3'-trailer sequence
-
-
?
IM3 + H2O
?
-
pre-tRNAIle with UUG 3'-trailer sequence
-
-
?
IT1 + H2O
?
-
pre-tRNAIle with CCG and 50 nucleotide 3'-trailer sequence
-
-
?
IT1 + H2O
?
-
pre-tRNAIle with CCG and 50 nucleotide 3'-trailer sequence
-
-
?
IT1 + H2O
?
-
pre-tRNAIle with CCG and 50 nucleotide 3'-trailer sequence
-
-
?
IT2 + H2O
?
-
pre-tRNAIle with CUG and 50 nucleotide 3'-trailer sequence
-
-
?
IT2 + H2O
?
-
pre-tRNAIle with CUG and 50 nucleotide 3'-trailer sequence
-
-
?
IT2 + H2O
?
-
pre-tRNAIle with CUG and 50 nucleotide 3'-trailer sequence
-
-
?
IT3 + H2O
?
-
pre-tRNAIle with UUG and 50 nucleotide 3'-trailer sequence
-
-
?
IT3 + H2O
?
-
pre-tRNAIle with UUG and 50 nucleotide 3'-trailer sequence
-
-
?
IT3 + H2O
?
-
pre-tRNAIle with UUG and 50 nucleotide 3'-trailer sequence
-
-
?
ITO + H2O
?
-
pre-tRNAIle with CCA and 50 nucleotide 3'-trailer sequence
-
-
?
ITO + H2O
?
-
pre-tRNAIle with CCA and 50 nucleotide 3'-trailer sequence
-
-
?
ITO + H2O
?
-
pre-tRNAIle with CCA and 50 nucleotide 3'-trailer sequence
-
-
?
pre-tRNA + H2O
?
-
the glycine/proline-rich ZiPD exosite of tRNase Z takes part in the pre-tRNA binding, it is a flexible arm which protrudes from the main protein body
-
-
?
pre-tRNA + H2O
?
-
the glycine/proline-rich ZiPD exosite of tRNase Z takes part in the pre-tRNA binding, it is a flexible arm which protrudes from the main protein body
-
-
?
pre-tRNA + H2O
?
-
the glycine/proline-rich ZiPD exosite of tRNase Z takes part in the pre-tRNA binding, it is a flexible arm which protrudes from the main protein body
-
-
?
pre-tRNA + H2O
?
-
cleaves intron-containing tRNA precursors and 5'-extended pre-tRNAs
-
-
?
pre-tRNA + H2O
?
-
cleaves pre-tRNAs 3' to the discriminator. Pre-tRNA with 3'-trailers harboring partial CCA motifs (C and CC) are cleaved in vitro by Trz 3' to C and CC, respectively, whereas CCA-containing pre-tRNAs are not cleaved, but are bound to the enzyme
-
-
?
pre-tRNA + H2O
?
-
the TM-type exosite of tRNase Z containing a cluster of 4-5 basic amino acid residues takes part in the pre-tRNA binding, it is a flexible arm which protrudes from the main protein body
-
-
?
pre-tRNA-CCA-trailer + H2O
?
-
3'-extended yeast pre-tRNAPhe
-
-
?
pre-tRNA-CCA-trailer + H2O
?
-
3'-extended yeast pre-tRNAPhe
-
-
?
pre-tRNA-CCAOH-trailer + H2O
?
-
3'-mature chloroplast pre-tRNAPhe
-
-
?
pre-tRNA-CCAOH-trailer + H2O
?
-
3'-mature chloroplast pre-tRNAPhe
-
-
?
pre-tRNA-CCAOH-trailer + H2O
?
-
3'-mature chloroplast pre-tRNAPhe
-
-
?
pre-tRNAAla + H2O
?
-
-
-
?
pre-tRNAAla + H2O
?
-
-
-
?
pre-tRNAarg + H2O
?
the new substrate pre-tRNAArg reveals kinetic parameters with wild type tRNase Z similar to those reported for pre-tRNAHis, enzyme concentration of 25 pM
-
-
?
pre-tRNAarg + H2O
?
-
-
-
-
?
pre-tRNAarg + H2O
?
-
cleavage by full-length tRNase ZL and DELTA30 tRNase ZL with the same efficiency
-
-
?
pre-tRNAarg + H2O
?
-
the enzyme catalyzes endonucleolytic tRNA 3' processing
-
-
?
pre-tRNAarg + H2O
?
-
-
-
-
?
pre-tRNAarg + H2O
?
-
-
-
-
?
pre-tRNACys + H2O
?
-
-
-
?
pre-tRNACys + H2O
?
-
-
-
?
pre-tRNACys + H2O
?
-
-
-
-
?
pre-tRNAGlu + H2O
?
-
-
-
-
?
pre-tRNAGlu + H2O
?
-
-
-
-
?
pre-tRNAHis + H2O
?
-
-
-
?
pre-tRNAHis + H2O
?
the nuclear tRNA is processed to an mature tRNA of 72 nucleotides and a 3' trailer of 32 nucleotides
-
-
?
pre-tRNAHis + H2O
?
-
-
-
-
?
pre-tRNAIle + H2O
?
the mitochondrial tRNA is processed to an mature tRNA of 65 nucleotides and a 3' trailer of 75 nucleotides
-
-
?
pre-tRNAIle + H2O
?
-
-
-
-
?
pre-tRNAIle + H2O
?
-
the enzyme catalyzes endonucleolytic tRNA 3' processing
-
-
?
pre-tRNALys + H2O
?
-
-
-
-
?
pre-tRNALys + H2O
?
-
the enzyme catalyzes endonucleolytic tRNA 3' processing
-
-
?
pre-tRNALys + H2O
?
-
-
-
-
?
pre-tRNALys + H2O
?
-
-
-
-
?
pre-tRNATyr + H2O
?
-
-
-
?
pre-tRNATyr + H2O
?
-
-
-
?
pre-tRNATyr + H2O
?
-
-
-
?
pre-tRNATyr + H2O
?
-
-
-
-
?
pre-tRNATyr + H2O
?
-
the enzyme catalyzes endonucleolytic tRNA 3' processing
-
-
?
pre-tRNAVal + H2O
?
-
-
-
-
?
pre-tRNAVal + H2O
?
-
-
-
-
?
precursor tRNASer(UGA)-M + H2O
mature tRNASer(UGA)-M
-
overexpression of ELAC2 increases the abundance of mature tRNASer(UGA)-M in strain yYH1
-
-
?
precursor tRNASer(UGA)-M + H2O
mature tRNASer(UGA)-M
overexpression of wild-type trz1+ in yYH1 cells can cause an increase in nonsense suppression by tRNASer(UGA)-M1, but overexpression of trz1+ cannot overcome the requirement for Sla1p for maturation of tRNASer(UGA)-M
-
-
?
precursor tRNATyr + H2O
tRNA + 3' trailer
all four recombinant tRNase Z proteins have tRNA 3'-processing activity
-
-
?
precursor tRNATyr + H2O
tRNA + 3' trailer
all four recombinant tRNase Z proteins have tRNA 3'-processing activity. TrZL2 processes the precursor less efficiently, which may be due to the fact that this enzyme is difficult to express and only low amounts of recombinant protein can be obtained
-
-
?
R-C1 + H2O
?
-
-
-
-
?
R-C1 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-CCA19 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-CCA19 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-G1 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-G1 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-G3 + H2O
?
-
pre-tRNAArg construct
-
-
?
R-G3 + H2O
?
-
pre-tRNAArg construct
-
-
?
Rib3 + H2O
?
-
substrate with mature 5'tRNA and 3' unprocessed snoRNA
-
-
?
Rib3 + H2O
?
-
5' mature dicistronic tRNAGly-snoR43.1 precursor RNA
-
-
?
Rib4 + H2O
?
-
substrate with mature 5'tRNA
-
-
?
Rib4 + H2O
?
-
5' and 3' mature dicistronic tRNAGly-snoR43.1 precursor RNA
-
-
?
S5L + H2O
?
-
pre-tRNATyr construct with 5 base pairs in the acceptor stem
-
-
?
S5L + H2O
?
-
pre-tRNATyr construct with 5 base pairs in the acceptor stem
-
-
?
S5L + H2O
?
-
pre-tRNATyr construct with 5 base pairs in the acceptor stem
-
-
?
S6L + H2O
?
-
pre-tRNATyr construct with 6 base pairs in the acceptor stem
-
-
?
S6L + H2O
?
-
pre-tRNATyr construct with 6 base pairs in the acceptor stem
-
-
?
S6L + H2O
?
-
pre-tRNATyr construct with 6 base pairs in the acceptor stem
-
-
?
S7L + H2O
?
-
wild type pre-tRNAIle
-
-
?
S7L + H2O
?
-
wild type pre-tRNAIle
-
-
?
S7L + H2O
?
-
wild type pre-tRNAIle
-
-
?
S8L + H2O
?
-
pre-tRNATyr construct with 8 base pairs in the acceptor stem
-
-
?
S8L + H2O
?
-
pre-tRNATyr construct with 8 base pairs in the acceptor stem
-
-
?
S8L + H2O
?
-
pre-tRNATyr construct with 8 base pairs in the acceptor stem
-
-
?
S9L + H2O
?
-
pre-tRNATyr construct with 9 base pairs in the acceptor stem
-
-
?
S9L + H2O
?
-
pre-tRNATyr construct with 9 base pairs in the acceptor stem
-
-
?
S9L + H2O
?
-
pre-tRNATyr construct with 9 base pairs in the acceptor stem
-
-
?
t-RNAHis48 + H2O
?
-
-
-
-
?
t-RNAHis48 + H2O
?
-
-
-
-
?
t-RNAHis48 3'C + H2O
?
-
-
-
-
?
t-RNAHis48 3'C + H2O
?
-
-
-
-
?
t-RNAHis48 3'CC + H2O
?
-
-
-
-
?
t-RNAHis48 3'CC + H2O
?
-
-
-
-
?
T7M7-T3H + H2O
?
-
complex of SPH2 with 5' half tRNAArg
-
-
?
T7M7-T3H + H2O
?
-
complex of SPH2 with 5' half tRNAArg
-
-
?
T7M7-T7HM1 + H2O
?
-
complex of SPH2 with 5' half tRNAArg
-
-
?
T7M7-T7HM1 + H2O
?
-
complex of SPH2 with 5' half tRNAArg
-
-
?
T7M7-T7HM2 + H2O
?
-
complex of SPH2 with 5' half tRNAArg
-
-
?
T7M7-T7HM2 + H2O
?
-
complex of SPH2 with 5' half tRNAArg
-
-
?
Thermotoga maritima pre-tRNAArg(CCA) + H2O
?
weak cleavage
-
-
?
Thermotoga maritima pre-tRNAArg(CCA) + H2O
?
weak cleavage
-
-
?
Thermotoga maritima pre-tRNAArg(CCA) + H2O
?
cleaved very inefficiently. The cleavage site of pre-tRNAArg(CCA) is identified after C75
-
-
?
Thermotoga maritima pre-tRNAArg(CCA) + H2O
?
cleaved very inefficiently. The cleavage site of pre-tRNAArg(CCA) is identified after C75
-
-
?
Thermotoga maritima pre-tRNAArg(CCA) + H2O
?
cleavage is barely detected
-
-
?
Thermotoga maritima pre-tRNAArg(CCA) + H2O
?
cleavage is barely detected
-
-
?
Thermotoga maritima pre-tRNAArg(GUG) + H2O
?
efficient cleavage
-
-
?
Thermotoga maritima pre-tRNAArg(GUG) + H2O
?
efficient cleavage
-
-
?
Thermotoga maritima pre-tRNAArg(GUG) + H2O
?
weak cleavage
-
-
?
Thermotoga maritima pre-tRNAArg(GUG) + H2O
?
cleaved very inefficiently
-
-
?
Thermotoga maritima pre-tRNAArg(GUG) + H2O
?
cleaved very inefficiently
-
-
?
Thermotoga maritima pre-tRNAMet(CCA) + H2O
?
cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) are cleaved after C75 and after the discriminator, respectively
-
-
?
Thermotoga maritima pre-tRNAMet(CCA) + H2O
?
cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) are cleaved after C75 and after the discriminator, respectively
-
-
?
Thermotoga maritima pre-tRNAMet(UAG) + H2O
?
cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) were cleaved after C75 and after the discriminator, respectively
-
-
?
Thermotoga maritima pre-tRNAMet(UAG) + H2O
?
cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) were cleaved after C75 and after the discriminator, respectively
-
-
?
tRNA T3 + H2O
?
-
tRNA variant without the anticodon arm
-
-
?
tRNA T3 + H2O
?
-
without anticodon arm
-
-
?
tRNA T6 + H2O
?
-
tRNA variant without the anticodon arm
-
-
?
tRNA T6 + H2O
?
-
without anticodon and variable arm
-
-
?
tRNA(His48) + H2O
?
-
-
-
-
?
tRNA(His48) + H2O
?
-
-
-
-
?
usRNA1 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA1 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA1 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA1 + H2O
?
-
24 nt unstructured RNA, no cleavage observed
-
-
?
usRNA1 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA1 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA1 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA10 + H2O
?
-
40 nt unstructured RNA
-
-
?
usRNA10 + H2O
?
-
40 nt unstructured RNA
-
-
?
usRNA10 + H2O
?
-
40 nt unstructured RNA
-
-
?
usRNA2 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA2 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA2 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA3 + H2O
?
-
28 nt unstructured RNA
-
-
?
usRNA3 + H2O
?
-
28 nt unstructured RNA
-
-
?
usRNA3 + H2O
?
-
28 nt unstructured RNA
-
-
?
usRNA4 + H2O
?
-
39 nt unstructured RNA
-
-
?
usRNA4 + H2O
?
-
39 nt unstructured RNA
-
-
?
usRNA4 + H2O
?
-
39 nt unstructured RNA
-
-
?
usRNA5 + H2O
?
-
26 nt unstructured RNA
-
-
?
usRNA5 + H2O
?
-
26 nt unstructured RNA
-
-
?
usRNA5 + H2O
?
-
26 nt unstructured RNA
-
-
?
usRNA6 + H2O
?
-
26 nt unstructured RNA
-
-
?
usRNA6 + H2O
?
-
26 nt unstructured RNA
-
-
?
usRNA6 + H2O
?
-
26 nt unstructured RNA
-
-
?
usRNA7 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA7 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA7 + H2O
?
-
24 nt unstructured RNA
-
-
?
usRNA8 + H2O
?
-
43 nt unstructured RNA
-
-
?
usRNA8 + H2O
?
-
43 nt unstructured RNA
-
-
?
usRNA8 + H2O
?
-
43 nt unstructured RNA
-
-
?
usRNA9 + H2O
?
-
22 nt unstructured RNA
-
-
?
usRNA9 + H2O
?
-
22 nt unstructured RNA
-
-
?
usRNA9 + H2O
?
-
22 nt unstructured RNA
-
-
?
additional information
?
-
-
cannot cleave substrates carrying a 3'-CCA motif
-
-
?
additional information
?
-
-
involvement of tRNase Z in mRNA processing
-
-
?
additional information
?
-
-
RNase BN is active on both double- and single-stranded RNA but duplex RNA is preferred. Displays a profound base specificity, showing no activity on runs of C residues. Digestion by RNase BN leads to 3-mers as the limit products, but the rate slows on molecules shorter than 10 nucleotides in length. RNase BN acts as a distributive exoribonuclease on some substrates, releasing mononucleotides and a ladder of digestion products. RNase BN also cleaves endonucleolytically, releasing 3' fragments as short as 4 nucleotides
-
-
?
additional information
?
-
-
involvement of tRNase Z in pre-5S rRNA cleavage
-
-
?
additional information
?
-
-
overexpression of ELAC2 does not appreciably increase the levels of mature tRNALys(CUU) and tRNAVal(AAC)
-
-
?
additional information
?
-
-
PPM1F and DYNC1H1 mRNAs are genuine targets of tRNase ZL guided by 5'-half-tRNAGlu and the 28S rRNA fragment, respectively
-
-
?
additional information
?
-
-
degraded RNA such as a 5'-half-tRNA and an rRNA fragment function as small guide RNA (sgRNA) guide the enzyme to target RNA
-
-
?
additional information
?
-
overexpression of trz1+ does not appreciably increase the levels of mature tRNALys(CUU) and tRNAVal(AAC)
-
-
?
additional information
?
-
overexpression of trz1+ does not appreciably increase the levels of mature tRNALys(CUU) and tRNAVal(AAC)
-
-
?
additional information
?
-
-
overexpression of trz1+ does not appreciably increase the levels of mature tRNALys(CUU) and tRNAVal(AAC)
-
-
?
additional information
?
-
-
tRNA 3'-processing endoribonuclease, member of the metallo-beta-lactamase superfamily
-
-
?
additional information
?
-
-
can cleave 3' to the CCA motif
-
-
?