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3.1.26.11: tRNase Z

This is an abbreviated version!
For detailed information about tRNase Z, go to the full flat file.

Word Map on EC 3.1.26.11

Reaction

endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule =

Synonyms

(tRNase) Z, 3 tRNase, 3' tRNase, 3'-tRNA processing endoribonuclease, 3'-tRNase, AthTrz1, AthTrz2, AthTrz3, AthTrz4, BsuTrz, CelTrz, DmeTrz, EcoTrz, EcoZ, Elac, Elac1, ELAC2, HsaTrz1, HsaTrz2, HvoTrz, long form of 3' tRNase, long form of tRNA 3' processing endoribonuclease, long form of tRNase Z, MjaTrz, nuclease, transfer ribonucleate maturation 3'-endoribo-(9CI), PaeTrz, pre-tRNA processing endoribonuclease, precursor tRNA 3'-end processing endoribonuclease, ribonuclease Z, RNase BN, RNase Z, RNase ZL, RNase ZS1, RNaseZ, RNZ, SceTrz, SpTrz1p, SpTrz2p, TM0207, TmaTrz, TMS5 protein, transfer RNA maturation endonuclease, tRNA 3 endonuclease, tRNA 3' processing endoribonuclease, tRNA 3'-processing enzyme tRNase Z, tRNA precursor-processing endoribonuclease, tRNase Z, tRNase Z2, tRNase ZL, tRNase ZS, tRNaseZ, tRnaseZL, tRnaseZS, Trz, TRZ1, Trz1p, TRZ2, TrZL1, TrZL2, TrZS1, TrZS2, YP_145327, YqiK, YqjK, zinc phosphodiesterase, zinc-dependent phosphodiesterase, ZiPD

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.11 tRNase Z

KM Value

KM Value on EC 3.1.26.11 - tRNase Z

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2 - 22.2
bis(p-nitrophenyl)phosphate
0.000016
pre-tRNA(Arg)
-
-
-
0.0000043
pre-tRNA(Val)
-
-
-
0.000022 - 0.000132
pre-tRNAArg
-
0.00047
pre-tRNAGlu-CCUN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.00155
pre-tRNAGlu-CUAN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.00096
pre-tRNAGlu-UUAN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.000029
pre-tRNAGly
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37°C
-
0.000018 - 0.000155
pre-tRNAHis
-
0.0000394
pre-tRNAHis-AUG
-
-
0.0000401
pre-tRNAHis-CCA
-
-
0.000048
pre-tRNAIle
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37°C
-
0.000024
pre-tRNALeu(UUR)
-
25 mM K-MOPS, 3 mM CaCl2, at pH 6.8 and 37°C
-
0.0000048
pre-tRNASer(UCN)
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000633
pre-tRNASer(UCN)7443C
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000069
pre-tRNASer(UCN)7444U
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000197
pre-tRNASer(UCN)7445C
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000855
pre-tRNASer(UCN)7445G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000108
pre-tRNASer(UCN)7510G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000076
pre-tRNASer(UCN)7511G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.000004
pre-tRNASer(UCN)7512G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.00044
R-ATM1
-
-
-
0.00065
R-ATM10
-
-
-
0.00068
R-ATM11
-
-
-
0.00032
R-ATM2
-
-
-
0.00059
R-ATM3
-
-
-
0.00066
R-ATM4
-
-
-
0.00057
R-ATM5
-
-
-
0.00058
R-ATM7
-
-
-
0.00052
R-ATM8
-
-
-
0.00063
R-ATM9
-
-
-
0.00041
R-ATW
-
-
-
0.00073
R-G10
-
-
-
0.0014
R-G13
-
-
-
0.001
R-G15
-
-
-
0.0012
R-G19
-
-
-
0.00085
R-G3
-
-
-
0.00078
R-G8
-
-
-
0.00083 - 0.00085
R-L0
-
0.00069
R-L3
-
-
-
0.0006
R-L6
-
-
-
0.0011
R-TASD2
-
-
-
0.0016
R-TSA2
-
-
-
0.0019
R-TSD1
-
-
-
0.00057
R-UUU
-
-
-
0.00002 - 0.00009
tRNA(His48)
-
0.00009
tRNAHis48 3'+ CC
-
CC is added to the 3'-end of tRNA
-
0.0022
trnI-Thr-CAAATG-trailer
-
the first cytosine is the primary anti-determinant for RNaseZ
-
0.0024
trnI-Thr-CCAATG-trailer
-
CCA motif inhibits RNaseZ cleavage
-
0.00097
trnI-Thr-TAAATG-trailer
-
native trailer sequence
-
0.00058
trnI-Thr-TCAATG-trailer
-
-
-
additional information
additional information
kinetics of tRNase Z processing in wild-type and variants shown, effects of substitutions in conserved regions on the C-side of motif II indicated, KM-values for tested variants similar to the mean wild-type KM value of 15 nM, residues involved with metal ion binding and catalysis not directly involved with substrate recognition and binding
-