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1.2 - 22.2
bis(p-nitrophenyl)phosphate
0.000016
pre-tRNA(Arg)
-
-
-
0.0000043
pre-tRNA(Val)
-
-
-
0.000022 - 0.000132
pre-tRNAArg
-
0.00047
pre-tRNAGlu-CCUN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.00155
pre-tRNAGlu-CUAN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.00096
pre-tRNAGlu-UUAN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.000029
pre-tRNAGly
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37°C
-
0.000018 - 0.000155
pre-tRNAHis
-
0.0000394
pre-tRNAHis-AUG
-
-
0.0000401
pre-tRNAHis-CCA
-
-
0.000048
pre-tRNAIle
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37°C
-
0.000024
pre-tRNALeu(UUR)
-
25 mM K-MOPS, 3 mM CaCl2, at pH 6.8 and 37°C
-
0.0000048
pre-tRNASer(UCN)
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000633
pre-tRNASer(UCN)7443C
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000069
pre-tRNASer(UCN)7444U
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000197
pre-tRNASer(UCN)7445C
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000855
pre-tRNASer(UCN)7445G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000108
pre-tRNASer(UCN)7510G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000076
pre-tRNASer(UCN)7511G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.000004
pre-tRNASer(UCN)7512G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.00002 - 0.00009
tRNA(His48)
-
0.00009
tRNAHis48 3'+ CC
-
CC is added to the 3'-end of tRNA
-
0.0022
trnI-Thr-CAAATG-trailer
-
the first cytosine is the primary anti-determinant for RNaseZ
-
0.0024
trnI-Thr-CCAATG-trailer
-
CCA motif inhibits RNaseZ cleavage
-
0.00097
trnI-Thr-TAAATG-trailer
-
native trailer sequence
-
0.00058
trnI-Thr-TCAATG-trailer
-
-
-
additional information
additional information
kinetics of tRNase Z processing in wild-type and variants shown, effects of substitutions in conserved regions on the C-side of motif II indicated, KM-values for tested variants similar to the mean wild-type KM value of 15 nM, residues involved with metal ion binding and catalysis not directly involved with substrate recognition and binding
-
1.2
bis(p-nitrophenyl)phosphate
-
AthTRZ1-P178A, mutant, bis(p-nitrophenyl)phosphate catalysis
1.8
bis(p-nitrophenyl)phosphate
-
AthTRZ1-R252G, mutant, bis(p-nitrophenyl)phosphate catalysis
3.5
bis(p-nitrophenyl)phosphate
-
AthTRZ1-E208A, mutant, bis(p-nitrophenyl)phosphate catalysis
6
bis(p-nitrophenyl)phosphate
-
AthTRZ1-C40G, mutant, bis(p-nitrophenyl)phosphate catalysis
6.5
bis(p-nitrophenyl)phosphate
-
AthTRZ1-F51L, mutant, bis(p-nitrophenyl)phosphate catalysis
8
bis(p-nitrophenyl)phosphate
-
AthTRZ1-G62V, mutant, bis(p-nitrophenyl)phosphate catalysis
8.5
bis(p-nitrophenyl)phosphate
-
AthTRZ1 wild-type, hydrolysis of phosphodiester bonds of bpNPP, KM value for AthTRZ1 two-fold increased relative to that of the tRNase Z of Escherichia coli
13.2
bis(p-nitrophenyl)phosphate
-
AthTRZ1-C25G, mutant, bis(p-nitrophenyl)phosphate catalysis
22.2
bis(p-nitrophenyl)phosphate
-
AthTRZ1-P64A, mutant, bis(p-nitrophenyl)phosphate catalysis
0.000022
pre-tRNAArg
-
25 mM K-MOPS, pH 6.75, 2 mM MgCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.000132
pre-tRNAArg
-
25 mM K-MOPS, pH 6.75, 1 mM MnCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.000018
pre-tRNAHis
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37°C
-
0.0000198
pre-tRNAHis
-
-
0.000155
pre-tRNAHis
25 mM Tris-HCl pH 7.0, 1 mM MgCl2, 1 mM DTT, 50 mM KCl at 28°C
-
0.00083
R-L0
-
-
-
0.00002
tRNA(His48)
-
at 37°C
-
0.00004
tRNA(His48)
-
at 28°C
-
0.00008
tRNA(His48)
-
-
-
0.00009
tRNA(His48)
-
C is added to the 3'-end of tRNA
-