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3.1.26.11: tRNase Z

This is an abbreviated version!
For detailed information about tRNase Z, go to the full flat file.

Word Map on EC 3.1.26.11

Reaction

endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule =

Synonyms

(tRNase) Z, 3 tRNase, 3' tRNase, 3'-tRNA processing endoribonuclease, 3'-tRNase, AthTrz1, AthTrz2, AthTrz3, AthTrz4, BsuTrz, CelTrz, DmeTrz, EcoTrz, EcoZ, Elac, Elac1, ELAC2, HsaTrz1, HsaTrz2, HvoTrz, long form of 3' tRNase, long form of tRNA 3' processing endoribonuclease, long form of tRNase Z, MjaTrz, nuclease, transfer ribonucleate maturation 3'-endoribo-(9CI), PaeTrz, pre-tRNA processing endoribonuclease, precursor tRNA 3'-end processing endoribonuclease, ribonuclease Z, RNase BN, RNase Z, RNase ZL, RNase ZS1, RNaseZ, RNZ, SceTrz, SpTrz1p, SpTrz2p, TM0207, TmaTrz, TMS5 protein, transfer RNA maturation endonuclease, tRNA 3 endonuclease, tRNA 3' processing endoribonuclease, tRNA 3'-processing enzyme tRNase Z, tRNA precursor-processing endoribonuclease, tRNase Z, tRNase Z2, tRNase ZL, tRNase ZS, tRNaseZ, tRnaseZL, tRnaseZS, Trz, TRZ1, Trz1p, TRZ2, TrZL1, TrZL2, TrZS1, TrZS2, YP_145327, YqiK, YqjK, zinc phosphodiesterase, zinc-dependent phosphodiesterase, ZiPD

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.11 tRNase Z

Turnover Number

Turnover Number on EC 3.1.26.11 - tRNase Z

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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.67
pre-tRNA(Arg)
-
-
-
0.27
pre-tRNA(Val)
-
-
-
0.021 - 6.18
pre-tRNAArg
-
0.14
pre-tRNAGlu-CCUN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.28
pre-tRNAGlu-CUAN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
2.25
pre-tRNAGlu-UUAN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.035
pre-tRNAGly
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37°C
-
0.083 - 0.237
pre-tRNAHis
-
0.365
pre-tRNAHis-AUG
-
-
0.0049
pre-tRNAHis-CCA
-
-
0.018
pre-tRNAIle
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37°C
-
0.25
pre-tRNALeu(UUR)
-
25 mM K-MOPS, 3 mM CaCl2, at pH 6.8 and 37°C
-
0.07
pre-tRNASer(UCN)
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.23
pre-tRNASer(UCN)7443C
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.153
pre-tRNASer(UCN)7444U
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.012
pre-tRNASer(UCN)7445C
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.345
pre-tRNASer(UCN)7445G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.05
pre-tRNASer(UCN)7510G, pre-tRNASer(UCN)7511G, pre-tRNASer(UCN)7512G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
additional information
additional information
-
turnover rate of AthTRZ1 eight-fold reduced if compared to tRNAse Z of Escherichia coli
-