3.1.22.1: deoxyribonuclease II

This is an abbreviated version, for detailed information about deoxyribonuclease II, go to the full flat file.

Reaction

endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products =

Synonyms

acid deoxyribonuclease, acid DNase, deoxyribonuclease II, deoxyribonuclease IIalpha, deoxyribonuclease IIbeta, deoxyribonucleate 3'-nucleotidohydrolase, DNase 2, DNase 2a, DNase II, DNase II homolog, DNase IIalpha, DNase IIb, DNase IIbeta, EC 3.1.4.6, L-DNase II, LEI, LEI-derived DNase II, LEI/L-DNase II, leukocyte elastase inhibitor-derived DNase II, leukocyte elastase inhibitor/LEI-derived DNase II, lysosomal DNase II, Nuc-1, NUC-1 apoptotic nuclease, pancreatic DNase II, plancitoxin 1, R31240_2

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.22 Endodeoxyribonucleases producing 3'-phosphomonoesters
                3.1.22.1 deoxyribonuclease II

Purification

Purification on EC 3.1.22.1 - deoxyribonuclease II

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by His-select cartridges
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commercially available DNase II further purified by affinity chromatography
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commercially available DNase II further purified by chromatography steps to homogeneity
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near homogeneity by 3step chromatography techniques
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near homogeneity by 5step chromatography techniques
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near homogeneity by chromatography steps
near homogeneity by chromatography steps and isoelectric focusing
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partial
partial purification using Con A Sepharose
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to homogeneity by 4-6step chromatography techniques
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to homogeneity by 4step chromatography techniques
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to homogeneity by 6step chromatography techniques
to homogeneity by chromatography steps and isoelectric focusing
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to homogeneity by chromatography techniques
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to homogeneity by gel filtration, 74.3fold purified
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to homogeneity by sucrose centrifugation steps and chromatography steps
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