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3.1.22.1: deoxyribonuclease II

This is an abbreviated version!
For detailed information about deoxyribonuclease II, go to the full flat file.

Word Map on EC 3.1.22.1

Reaction

endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products =

Synonyms

acid deoxyribonuclease, acid DNase, crn-7, deoxyribonuclease II, deoxyribonuclease IIalpha, deoxyribonuclease IIbeta, deoxyribonucleate 3'-nucleotidohydrolase, DNase 2, DNase 2a, DNase II, DNase II homolog, DNase II-1, DNase II-7, DNase IIalpha, DNase IIb, DNase IIbeta, EC 3.1.4.6, L-DNase II, LEI, LEI-derived DNase II, LEI/L-DNase II, leukocyte elastase inhibitor-derived DNase II, leukocyte elastase inhibitor/LEI-derived DNase II, lysosomal DNase II, Nuc-1, NUC-1 apoptotic nuclease, pancreatic DNase II, plancitoxin 1, R31240_2, Sjda, TsDNase II-1, TsDNase II-7

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.22 Endodeoxyribonucleases producing 3'-phosphomonoesters
                3.1.22.1 deoxyribonuclease II

Cloned

Cloned on EC 3.1.22.1 - deoxyribonuclease II

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA of pDNase II cloned in the pcDNA3 vector and transfected into HEK 293T cells
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DNA fragment containing the entire coding sequence of DNase II cDNA amplified and ligated into pcDNA3.1(+). Mutants corresponding to each single nucleotide polymorphism expressed in COS-7 cell
Escherichia coli BL21(DE3) pLysS strain transformed with a pET 23d(+) plasmid coding for LEI with a 6x His tag. PZeo-LEI wild-type transfected into HeLa cells, MEF cell lines or BHK-21 cells
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expression in Chinese hamster ovary cells
expression in COS-7 cells
expression in Escherichia coli
expression in Escherichia coli DH5alpha
expression in human embryonic kidney 293 cells
LEI cDNA subcloned into pZeoSV2+, into pET23d+ in frame with the His-tag, and into pDsRed2-C1 plasmid in frame with the red fluorescent protein fused to its N-term. Expression in Escherichia coli strain BL21(DE3) pLysS. BHK cells transfected with a pZeo vector containing the wild-type or a punctually mutated LEI cDNA
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MEF-/- cells null for enzyme expression and activity are transiently transfected with plasmid-expression constructs encoding wild-type human DNase IIalpha, pD2, or mutant forms
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nuc-1, crn-7 or crn-6 coding sequence without a stop codon amplified and inserted into the pPD95.79 vector. Transcriptional reporter constructs containing the promoter of the DNase II gene fused to GFP with four tandem copies of the nuclear localization signal (NLS) injected into wild-type animals (embryos, larvae and adults)
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Sp1 and Sp3 play a pivotal role in the transcriptional activation of DNase II in HL-60 cells during phorbol 12-myristate-13-acetate induced differentiation
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the C07B5.5 gene encodes the NUC-1 apoptotic nuclease
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the gene encoding DNase IIbeta has identical splice sites to DNase IIalpha
the subsequent targeted disruption of the DNase II gene confirms the requirement of DNase II activity within phagocytic cells
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the wild-type and mutant enzyme cDNA plasmid are transiently transfected in HCT116 cells
wild-type or mutant LEI expressed in BHK-21 cells. 6xHis tagged wild-type and mutant LEI expressed from pET 23d(+) plasmid in Escherichia coli BL21(DE3)pLysS strain. Wild-type LEI expressed in HeLa cells
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