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2-mercaptoethanol
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little effect on activity at 10 mM
actinomycin D
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strong inhibition, coupling to the substrate not to the enzyme
aurintricarboxylic acid
-
-
copper sulphate
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suppresses enzyme activity in hemocytes
DNA
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inhibitory above 0.4 mg/ml
EDTA
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dialysis of IgGs from rabbits immunized with DNase II against a buffer containing EDTA or addition of EDTA to the reaction mixture leads to a complete disappearance of DNase activity
Fe3+
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complete inhibition at 0.1 mM
growth hormone
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in mice fed a 20% protein diet, growth hormone treatment decreases elevated activites of DNase II and RNase
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HPO42-
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slightly inhibitory above pH 5.0
human growth hormone
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treatment of post-weaning mice results in 63% inhibition in gastrocnemius muscle
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iodoacetamide
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slight inhibitory
Mn(CH3COO)2
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32% inhibition at 20 mM
N-bromosuccinimide
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strong inhibitory
N-ethylmaleimide
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little effect on activity at 10 mM
Protein inhibitor
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inhibition below pH 5.6, forming a DNase-inhibitor complex
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RNAi
Drosophila sp. (in: flies)
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expression of a DNase IIRNAi construct in flies results in a significant decrease in total hemocyte numbers
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rRNA
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specific competitive inhibition
succinate buffer
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activity less than 3% of that in acetate buffer at pH 5.0
Zn(CH3COO)2
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67% inbhibition at 20 mM
bovine serum albumin
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90% inhibition at 0.015 mg/ml, addition of inhibitor changes pH-activity curve, optimal pH shifted to the alkaline side, DNA substrate compete with inhibitor and reverses inhibitor action
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bovine serum albumin
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90% inhibition at 0.015 mg/ml, addition of inhibitor changes pH-activity curve, optimal pH shifted to the alkaline side, DNA substrate compete with inhibitor and reverses inhibitor action
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Co2+
-
complete inhibition
Co2+
-
inhibition above 10 mM
Cu2+
inhibited by copper ions and to a lesser extent manganese ions, but not inhibited by calcium, magnesium or zinc ions
Cu2+
-
complete inhibition
iodoacetate
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43% inhibition at 1 mM, 93% inhibition at 5 mM
iodoacetate
-
85% inhibition at 5 mM
iodoacetate
-
complete inhibition at 0.2 mM
iodoacetate
-
strongly inhibitory
Mg2+
about 35% inhibition at 0.1 mM, complete inhibition at 10 mM
Mg2+
-
inhibition above 10 mM
Mg2+
-
addition of Mg2+ ions to the dialyzed preparations of IgGs leads to a reduction of the IgG DNase activity
Mg2+
-
inhibition above 10 mM
Mg2+
-
slightly inhibitory at 10 mM and pH 4.5, inhibitory effects depending on enzyme purity
Mn2+
inhibited by copper ions and to a lesser extent manganese ions, but not inhibited by calcium, magnesium or zinc ions
Mn2+
-
inhibition above 10 mM
Na2SO4
-
above 50 mM
NaCl
-
above 250 mM
NaCl
-
decreasing activity with increasing ionic strength
SO42-
-
-
SO42-
-
slightly inhibitory
SO42-
-
68% inhibition at 20 mM
SO42-
-
very strong inhibition, particulary above pH 4.5
tRNA
-
from E. coli, little effect on activity
tRNA
-
specific competitive inhibition
tunicamycin
inhibits the N-linked glycosylation and virtually abolishes the enzyme activity
tunicamycin
-
the N-glycosylation inhibitor diminishes the activity
additional information
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not: EDTA
-
additional information
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caspases inhibitors have no effect on acid endonucleases, and no effect on apoptosis
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additional information
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despite having putative poly(ADP-ribose)-acceptor sites, LEI/L-DNase II is neither significantly poly(ADP-ribosyl)ated nor inhibited by PARP-1 during caspase-independent apoptosis
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