3.1.22.1: deoxyribonuclease II

This is an abbreviated version, for detailed information about deoxyribonuclease II, go to the full flat file.

Reaction

endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products =

Synonyms

acid deoxyribonuclease, acid DNase, deoxyribonuclease II, deoxyribonuclease IIalpha, deoxyribonuclease IIbeta, deoxyribonucleate 3'-nucleotidohydrolase, DNase 2, DNase 2a, DNase II, DNase II homolog, DNase IIalpha, DNase IIb, DNase IIbeta, EC 3.1.4.6, L-DNase II, LEI, LEI-derived DNase II, LEI/L-DNase II, leukocyte elastase inhibitor-derived DNase II, leukocyte elastase inhibitor/LEI-derived DNase II, lysosomal DNase II, Nuc-1, NUC-1 apoptotic nuclease, pancreatic DNase II, plancitoxin 1, R31240_2

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.22 Endodeoxyribonucleases producing 3'-phosphomonoesters
                3.1.22.1 deoxyribonuclease II

Inhibitors

Inhibitors on EC 3.1.22.1 - deoxyribonuclease II

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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
little effect on activity at 10 mM
actinomycin D
-
strong inhibition, coupling to the substrate not to the enzyme
Alkylating agents
-
-
-
aurintricarboxylic acid
-
-
bovine serum albumin
-
Ca2+
-
strong inhibition
CaCl2
-
above 200 mM
copper sulphate
-
suppresses enzyme activity in hemocytes
Cu2+
-
complete inhibition
DNA
-
inhibitory above 0.4 mg/ml
EDTA
-
dialysis of IgGs from rabbits immunized with DNase II against a buffer containing EDTA or addition of EDTA to the reaction mixture leads to a complete disappearance of DNase activity
Fe3+
-
complete inhibition at 0.1 mM
growth hormone
-
in mice fed a 20% protein diet, growth hormone treatment decreases elevated activites of DNase II and RNase
-
HPO42-
-
slightly inhibitory above pH 5.0
human growth hormone
-
treatment of post-weaning mice results in 63% inhibition in gastrocnemius muscle
-
iodoacetamide
-
slight inhibitory
iodoacetate
KCl
-
above 300 mM
MgCl2
-
above 150 mM
Mn(CH3COO)2
-
32% inhibition at 20 mM
MnCl2
-
above 200 mM
N-bromosuccinimide
-
strong inhibitory
N-ethylmaleimide
-
little effect on activity at 10 mM
Na2SO4
Protein inhibitor
-
inhibition below pH 5.6, forming a DNase-inhibitor complex
-
RNAi
-
expression of a DNase IIRNAi construct in flies results in a significant decrease in total hemocyte numbers
-
rRNA
-
specific competitive inhibition
SO42-
succinate buffer
-
activity less than 3% of that in acetate buffer at pH 5.0
-
tunicamycin
Zn(CH3COO)2
-
67% inbhibition at 20 mM
Zn2+
-
strong inhibition
ZnCl2
-
above 150 mM
additional information
-