3.1.22.1: deoxyribonuclease II

This is an abbreviated version, for detailed information about deoxyribonuclease II, go to the full flat file.

Reaction

endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products =

Synonyms

acid deoxyribonuclease, acid DNase, deoxyribonuclease II, deoxyribonuclease IIalpha, deoxyribonuclease IIbeta, deoxyribonucleate 3'-nucleotidohydrolase, DNase 2, DNase 2a, DNase II, DNase II homolog, DNase IIalpha, DNase IIb, DNase IIbeta, EC 3.1.4.6, L-DNase II, LEI, LEI-derived DNase II, LEI/L-DNase II, leukocyte elastase inhibitor-derived DNase II, leukocyte elastase inhibitor/LEI-derived DNase II, lysosomal DNase II, Nuc-1, NUC-1 apoptotic nuclease, pancreatic DNase II, plancitoxin 1, R31240_2

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.22 Endodeoxyribonucleases producing 3'-phosphomonoesters
                3.1.22.1 deoxyribonuclease II

Molecular Weight

Molecular Weight on EC 3.1.22.1 - deoxyribonuclease II

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
gel filtration, SDS-PAGE
31000
-
gel filtration
32000
-
1 * 8000-10000 + 1 * 32000 + 1 * ?, cDNA and protein sequence analysis
34000
-
x * 34000, SDS-PAGE
37000
-
unglycosylated form of wild-type enzyme and N69Q mutant upon tunicamycin treatment
38030
-
calculation from cDNA sequence
38210
-
sequence analysis
40000
-
-
41000
-
gel filtration
42000 - 44000
-
SDS-PAGE, mature protein
43000
-
mutants N86Q, N212Q, N266Q, N290Q. It appears that the four sites each contribute to glycosylation and no additional glycosylation sites are responsible for the observed size
45000 - 46000
-
gel filtration, SDS-PAGE
46500
-
Western blot analysis or calculated (addition of the mass of sugar contents to the sequence-derived mass)
47000
-
D107N mutant, addition of glycosylation site