3.1.22.1: deoxyribonuclease II

This is an abbreviated version, for detailed information about deoxyribonuclease II, go to the full flat file.

Reaction

endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products =

Synonyms

acid deoxyribonuclease, acid DNase, deoxyribonuclease II, deoxyribonuclease IIalpha, deoxyribonuclease IIbeta, deoxyribonucleate 3'-nucleotidohydrolase, DNase 2, DNase 2a, DNase II, DNase II homolog, DNase IIalpha, DNase IIb, DNase IIbeta, EC 3.1.4.6, L-DNase II, LEI, LEI-derived DNase II, LEI/L-DNase II, leukocyte elastase inhibitor-derived DNase II, leukocyte elastase inhibitor/LEI-derived DNase II, lysosomal DNase II, Nuc-1, NUC-1 apoptotic nuclease, pancreatic DNase II, plancitoxin 1, R31240_2

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.22 Endodeoxyribonucleases producing 3'-phosphomonoesters
                3.1.22.1 deoxyribonuclease II

Substrates Products

Substrates Products on EC 3.1.22.1 - deoxyribonuclease II

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
bis(p-nitrophenyl) phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate:
show the reaction diagram
-
slow hydrolysis at optimal pH from 5.6 to 5.9
-
?
calf thymus DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
show the reaction diagram
-
-
-
-
?
chromatin + H2O
oligonucleotides + ?
show the reaction diagram
deoxyribonucleoside 5'-phosphates p-nitrophenyl esters + H2O
p-nitrophenol + deoxyribonucleoside 5'-phosphates
show the reaction diagram
-
-
-
?
DNA + H2O
3' phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
3'-phosphooligonucleotide
show the reaction diagram
DNA + H2O
3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
show the reaction diagram
DNA + H2O
?
show the reaction diagram
DNA + H2O
oligonucleotides + ?
show the reaction diagram
dsDNA + H2O
oligonucleotides + ?
show the reaction diagram
-
velocity of DNA degradtion is four times higher four dsDNA than for ssDNA
-
-
?
duplex-oligonucleotide + H2O
?
show the reaction diagram
-
20- and 16mers with specific sequences and structures like hairpin and duplex DNA, can cut bonds in a loop at nearly the same rate as in duplex DNA, doesn't require phosphate exposure or a double-stranded track
-
?
d[ApAp(S)ApA] + H2O
d[Ap(S)ApA] + 3'-dAMP
show the reaction diagram
-
digestion of Rp- and Sp-isomers
after 2 h incubation, prolonged incubation leads to d[Ap(S)Ap]-Rp-isomer, dA and d[Ap(S)Ap]-Sp-isomer. Does not hydrolyse phosphorothioate internucleotidic linkage of either configuration
?
d[ApApApA] + H2O
d[ApApA] + 3'-dAMP
show the reaction diagram
-
-
after 60 min of incubation, prolonged incubation leads to 3'-dAMP and dA end products in a 3 : 1 ratio
?
linearized plasmid DNA + H2O
oligonucleotides + ?
show the reaction diagram
-
-
-
?
nuclei + H2O
oligonucleotides + ?
show the reaction diagram
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
?
plasmid DNA + H2O
?
show the reaction diagram
salmon testicular DNA + H2O
?
show the reaction diagram
-
-
-
-
?
ssDNA + H2O
oligonucleotides + ?
show the reaction diagram
-
velocity of DNA degradtion is four times higher four dsDNA than for ssDNA
-
-
?
supercoiled plasmid DNA + H2O
linearized plasmid DNA + ?
show the reaction diagram
-
-
-
?
supercoiled plasmid DNA + H2O
relaxed plasmid DNA + ?
show the reaction diagram
additional information
?
-