3.1.16.1: spleen exonuclease
This is an abbreviated version!
For detailed information about spleen exonuclease, go to the full flat file.
Word Map on EC 3.1.16.1
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3.1.16.1
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flap
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endonuclease-1
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structure-specific
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okazaki
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nucleases
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micrococcal
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snake
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polynucleotide
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5'-flaps
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32p-postlabeling
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3'-monophosphate
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long-patch
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lagging-strand
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2'-deoxyguanosine
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5'-exonuclease
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exonucleolytic
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dinucleoside
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fen1
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analysis
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internucleotide
- 3.1.16.1
- flap
-
endonuclease-1
-
structure-specific
-
okazaki
- nucleases
-
micrococcal
-
snake
- polynucleotide
-
5'-flaps
-
32p-postlabeling
- 3'-monophosphate
-
long-patch
-
lagging-strand
- 2'-deoxyguanosine
- 5'-exonuclease
-
exonucleolytic
-
dinucleoside
- fen1
- analysis
-
internucleotide
Reaction
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates =
Synonyms
3'-5' exonuclease, 3'-exonuclease, 3'-nucleotide phosphodiesterase, 5' -> 3' exonuclease Xrn2, 5' 3'-exonuclease, 5'-to-3' exonuclease, 5->3 exoribonuclease, Ape2, beef spleen exonuclease, BSEase, BSP, EC 3.1.4.18, exonuclease, FEN-1, flap endonuclease-1, More, NPP1, nucleotide pyrophosphatase/phosphodiesterase 1, phosphodiesterase II, spleen phosphodiesterase, Trm2p, XRNA, XRNB, XRNC, XRND
ECTree
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Inhibitors
Inhibitors on EC 3.1.16.1 - spleen exonuclease
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dimethyl sulfate
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inhibition after methylation of 2'-deoxyribooligonucleotides with dimethyl sulfate
glycosylation
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no activity with glycosylated oligonucleotides obtained from T4 phage DNA
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KCl
FEN-1 retains up to approximately 70.0% of its endonuclease at 0-50.0 mM, between 75 and 100 mM FEN-1 displays a significant reduction in activity and at the highest salt concentrations between 125-200 mM almost a complete ablation of activity occurres
Pentamidine
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endo-exonuclease inhibitor, inhibits DNase activity of CBP-Trm2p
RNAi
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down-regulation of expression by tetracycline-inducible RNA interference
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single-stranded DNA-binding protein
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i.e. StoSSB, from the hyperthermophilic archaeon Sulfolobus tokodaii, StoSSB inhibits the exonuclease activity of StoNurA on ssDNA and dsDNA
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slightly greater inhibition of rat than of guinea-pig enzyme at sodium succinate buffer concentrations greater 0.4 M, also markedly inhibition by 6 M NaCl and 2 M ammonium sulfate
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high ionic strength
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slightly greater inhibition of rat than of guinea-pig enzyme at sodium succinate buffer concentrations greater 0.4 M, also markedly inhibition by 6 M NaCl and 2 M ammonium sulfate
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Mg2+
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almost no degradation of tRNA at higher concentrations with 1 Mg2+ per nucleotide phosphate
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at 100 mM Ape2 exhibits only 20% of its exonuclease activity, in the presence of 200 mM salt almost no exonuclease activity
NaCl
FEN-1 retains up to approximately 70.0% of its endonuclease at 0-50.0 mM, between 75 and 100 mM FEN-1 displays a significant reduction in activity and at the highest salt concentrations between 125-200 mM almost a complete ablation of activity occurres
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after 2 h incubation almost 80% of activity with 1.0 M, 40% with 2.0 M, no activity with 5.0 M