Application | Comment | Organism |
---|---|---|
additional information | Plasmodium FEN-1s have enzymatic activities similar to other species but contain extended C-termini and a more internally located proliferating cell nuclear antigen-binding site. FEN-1 homologs exhibit both endonuclease and exonuclease activities in vitro | Plasmodium falciparum |
additional information | Plasmodium FEN-1s have enzymatic activities similar to other species but contain extended C-termini and a more internally located proliferating cell nuclear antigen-binding site. FEN-1 homologs exhibit both endonuclease and exonuclease activities in vitro | Plasmodium yoelii |
Cloned (Comment) | Organism |
---|---|
FEN-1 cloned into the pCAL-n-FLAG expression vector and expressed in Escherichia coli BLR(DE3)pLysS cells. C-terminal truncation mutant cloned into pET24d(+) and expressed in Escherichia coli BL21(DE3)CodonPlus-RIL | Plasmodium falciparum |
HIS6 tagged gene subcloned into pET24d(+) and expressed in Escherichia coli BL21(DE3)CodonPlus-RIL | Plasmodium yoelii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | C-terminal truncation mutant lacking the terminal 250 amino acids has endonuclease activity that is ca. 130fold greater than full-length FEN-1 | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | FEN-1 retains up to approximately 70.0% of its endonuclease at 0-50.0 mM, between 75 and 100 mM FEN-1 displays a significant reduction in activity and at the highest salt concentrations between 125-200 mM almost a complete ablation of activity occurres | Plasmodium falciparum | |
Mg2+ | inhibits above 20 mM | Plasmodium falciparum | |
NaCl | FEN-1 retains up to approximately 70.0% of its endonuclease at 0-50.0 mM, between 75 and 100 mM FEN-1 displays a significant reduction in activity and at the highest salt concentrations between 125-200 mM almost a complete ablation of activity occurres | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000038 | - |
Flap34endo | - |
Plasmodium falciparum | |
0.0000045 | - |
Flap34endo | C-terminal truncation mutant | Plasmodium falciparum | |
0.0000066 | - |
Flap34endo | - |
Plasmodium yoelii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | expression of endogenous FEN-1 occurres in the ring, trophozoite, and schizont stages | Plasmodium falciparum | - |
- |
nucleus | - |
Plasmodium falciparum | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | yields the highest activity for FEN-1 | Plasmodium yoelii | |
Mg2+ | yields the highest activity for FEN-1, activity is stimulated above 0.1 mM, reaching a maximum at ca. 10 mM | Plasmodium falciparum | |
Mn2+ | yields the highest activity for FEN-1 | Plasmodium falciparum | |
additional information | Ni2+ or Mn2+ does not have a pronounced stimulatory effect | Plasmodium yoelii | |
Ni2+ | minimally stimulates activity | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
C-terminal truncation mutant, Western blot analysis | Plasmodium falciparum |
54500 | - |
sequence analysis | Plasmodium yoelii |
75000 | - |
sequence analysis, Western blot analysis | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q9GZ01 | - |
- |
Plasmodium falciparum FCR-3/C5 | Q9GZ01 | - |
- |
Plasmodium yoelii | Q7RME3 | - |
- |
Purification (Comment) | Organism |
---|---|
by cation exchange column and on Ni-NTA column | Plasmodium yoelii |
FEN-1 purified by cation exchange column followed by a calmodulin affinity resin, more than 95% pure, C-terminal truncation mutant purified on Ni-NTA column and by cation exchange column, more than 95% pure | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Plasmodium falciparum | deoxyribonucleoside 3'-phosphate + ? | - |
? | |
DNA + H2O | - |
Plasmodium falciparum FCR-3/C5 | deoxyribonucleoside 3'-phosphate + ? | - |
? | |
Flap34endo + H2O | - |
Plasmodium falciparum | ? | - |
? | |
Flap34endo + H2O | - |
Plasmodium yoelii | ? | - |
? | |
Flap34endo + H2O | - |
Plasmodium falciparum FCR-3/C5 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FEN-1 | - |
Plasmodium falciparum |
FEN-1 | - |
Plasmodium yoelii |
flap endonuclease-1 | - |
Plasmodium falciparum |
flap endonuclease-1 | - |
Plasmodium yoelii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
Flap34endo | C-terminal truncation mutant | Plasmodium falciparum | |
4.9 | - |
Flap34endo | - |
Plasmodium yoelii | |
9.1 | - |
Flap34endo | - |
Plasmodium falciparum |