3.1.16.1: spleen exonuclease
This is an abbreviated version!
For detailed information about spleen exonuclease, go to the full flat file.
Word Map on EC 3.1.16.1
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3.1.16.1
-
flap
-
endonuclease-1
-
structure-specific
-
okazaki
-
nucleases
-
micrococcal
-
snake
-
polynucleotide
-
5'-flaps
-
32p-postlabeling
-
3'-monophosphate
-
long-patch
-
lagging-strand
-
2'-deoxyguanosine
-
5'-exonuclease
-
exonucleolytic
-
dinucleoside
-
fen1
-
analysis
-
internucleotide
- 3.1.16.1
- flap
-
endonuclease-1
-
structure-specific
-
okazaki
- nucleases
-
micrococcal
-
snake
- polynucleotide
-
5'-flaps
-
32p-postlabeling
- 3'-monophosphate
-
long-patch
-
lagging-strand
- 2'-deoxyguanosine
- 5'-exonuclease
-
exonucleolytic
-
dinucleoside
- fen1
- analysis
-
internucleotide
Reaction
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates =
Synonyms
3'-5' exonuclease, 3'-exonuclease, 3'-nucleotide phosphodiesterase, 5' -> 3' exonuclease Xrn2, 5' 3'-exonuclease, 5'-to-3' exonuclease, 5->3 exoribonuclease, Ape2, beef spleen exonuclease, BSEase, BSP, EC 3.1.4.18, exonuclease, FEN-1, flap endonuclease-1, More, NPP1, nucleotide pyrophosphatase/phosphodiesterase 1, phosphodiesterase II, spleen phosphodiesterase, Trm2p, XRNA, XRNB, XRNC, XRND
ECTree
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Temperature Stability
Temperature Stability on EC 3.1.16.1 - spleen exonuclease
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35 - 60
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incubation at pH 5.7, 10 min: 90% of initial activity at 35°C, 10% at 60°C
60
61
-
lost of 50% of activity after heating of partially purified enzyme for 5 min
64
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lost of 50% of activity after heating of partially purified enzyme for 5 min
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almost complete inactivation after incubation for 20 min at acidic or neutral pH