Application | Comment | Organism |
---|---|---|
analysis | end group and sequential analysis of polynucleotides | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition | Bos taurus | |
Urea | 50% inhibition with 2.0 M | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
thymidine 3'-(4-nitrophenyl)phosphate | - |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates | also degradation of thymine and adenine polynucleotides to thymine and adenine | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.72 | - |
with polyadenylic acid as substrate | Bos taurus |
34 | - |
with thymidine 3'-(4-nitrophenyl)phosphate as substrate | Bos taurus |
43.3 | - |
with 5'-hydroxy-thymidine oligonucleotide TpT, lacking terminal phosphomonoester group, as substrate | Bos taurus |
43.3 | - |
with 5'-hydroxy-thymidine oligonucleotide TpTpTpT, lacking terminal phosphomonoester group, as substrate | Bos taurus |
43.3 | - |
with 5'-hydroxy-2'deoxy-cytosine oligonucleotide d-CpCpC, lacking terminal phosphomonoester group, as substrate | Bos taurus |
45 | - |
with 5'-hydroxy-thymidine oligonucleotide TpTp as substrate | Bos taurus |
58.3 | - |
with 5'-hydroxy-oligonucleotide ApUp as substrate | Bos taurus |
58.3 | - |
with 5'-hydroxy-adenine oligonucleotide ApApA, lacking terminal phosphomonoester group, as substrate | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-hydroxy oligonucleotides + H2O | 5'-hydroxy-thymidine oligonucleotides , 5'-hydroxy-adenine oligonucleotides, 5'-hydroxy-2'-deoxycytidine oligonucleotides and various other 5'-hydroxy- and 5'-hydroxy-2'deoxy oligonucleotides like ApUp, d-TpTpC | Bos taurus | nucleoside 3'-oligonucleotides + nucleoside 3'-phosphates | - |
r | |
polyadenylic acid + H2O | - |
Bos taurus | 5'-hydroxy-adenine oligonucleotides + 3'AMP | - |
r | |
RNA core + H2O | - |
Bos taurus | nucleoside 3'-phosphates | - |
? | |
thymidine 3'-(4-nitrophenyl)phosphate + H2O | - |
Bos taurus | thymidine 3'-phosphate + 4-nitrophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
spleen phosphodiesterase | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | 6.5 | 5.9 with almost 0.15 M ammonium succinate buffer, 6.5 with almost 0.3 M and thymidine 3'(4-nitrophenyl)phosphate as substrate | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
Urea | - |
Bos taurus |