3.1.16.1: spleen exonuclease
This is an abbreviated version!
For detailed information about spleen exonuclease, go to the full flat file.
Word Map on EC 3.1.16.1
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3.1.16.1
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flap
-
endonuclease-1
-
structure-specific
-
okazaki
-
nucleases
-
micrococcal
-
snake
-
polynucleotide
-
5'-flaps
-
32p-postlabeling
-
3'-monophosphate
-
long-patch
-
lagging-strand
-
2'-deoxyguanosine
-
5'-exonuclease
-
exonucleolytic
-
dinucleoside
-
fen1
-
analysis
-
internucleotide
- 3.1.16.1
- flap
-
endonuclease-1
-
structure-specific
-
okazaki
- nucleases
-
micrococcal
-
snake
- polynucleotide
-
5'-flaps
-
32p-postlabeling
- 3'-monophosphate
-
long-patch
-
lagging-strand
- 2'-deoxyguanosine
- 5'-exonuclease
-
exonucleolytic
-
dinucleoside
- fen1
- analysis
-
internucleotide
Reaction
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates =
Synonyms
3'-5' exonuclease, 3'-exonuclease, 3'-nucleotide phosphodiesterase, 5' -> 3' exonuclease Xrn2, 5' 3'-exonuclease, 5'-to-3' exonuclease, 5->3 exoribonuclease, Ape2, beef spleen exonuclease, BSEase, BSP, EC 3.1.4.18, exonuclease, FEN-1, flap endonuclease-1, More, NPP1, nucleotide pyrophosphatase/phosphodiesterase 1, phosphodiesterase II, spleen phosphodiesterase, Trm2p, XRNA, XRNB, XRNC, XRND
ECTree
3.1.16.1 spleen exonucleaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.1.16.1 - spleen exonuclease
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl thymidine 3'-phosphate + H2O
thymidine 3'-phosphate + 4-methylumbelliferone
-
-
-
?
5'-hydroxy oligonucleotides + H2O
nucleoside 3'-oligonucleotides + nucleoside 3'-phosphates
5-'deoxy-5'-chlorothymidine-3'-(4-nitrophenyl)phosphate + H2O
5'-deoxy-5'-chlorothymidine-3'-phosphate
-
-
-
?
mRNA + H2O
?
-
XRNA is important in the degradation of deadenylated mRNAs in trypanosomes
-
-
?
polynucleotides + H2O
phosphomononucleotides
-
the enzyme contributes to the turnover of messenger RNAs in eukaryotic cells
-
-
?
thymidine 3'-(2,4-dinitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 2,4-dinitrophenol
[PO/Mix-PS]-d[TPOCPOTPOTPOTPOTPOTPSC] + H2O
?
-
21% degraded after 2 hours, 35% degraded after 8 hours
-
-
?
[PO/Mix-PS]-d[TPOTPOTPOTPOTPOTPOTPOTPOTPOTPOTPST] + H2O
?
-
35% degraded after 2 hours, 75% degraded after 8 hours
-
-
?
[PO/RP-PS]-d[TPOCPOTPOTPOTPOTPOTPSC] + H2O
?
-
22% degraded after 2 hours, 45% degraded after 8 hours
-
-
?
[PO/SP-PS]-d[TPOCPOTPOTPOTPOTPOTPSC] + H2O
?
-
20% degraded after 2 hours, 25% degraded after 8 hours
-
-
?
[RP-PS]-d[T12] + H2O
?
-
25% degraded after 2 hours, 75% degraded after 8 hours
-
-
?
3'-phospho-2'-deoxyribooligonucleotide + H2O
2'-deoxyribonucleoside 3'-phosphate
-
dinucleotides
-
-
?
3'-phospho-2'-deoxyribooligonucleotide + H2O
2'-deoxyribonucleoside 3'-phosphate
-
-
-
?
3'-phospho-2'-deoxyribooligonucleotide + H2O
2'-deoxyribonucleoside 3'-phosphate
-
-
-
?
3'-phospho-2'-deoxyribooligonucleotide + H2O
2'-deoxyribonucleoside 3'-phosphate
-
average size 10-12, smallest 3
-
?
3'-phospho-2'-deoxyribooligonucleotide + H2O
2'-deoxyribonucleoside 3'-phosphate
-
methylated by dimethyl sulfate
-
?
3'-phospho-2'-deoxyribooligonucleotide + H2O
2'-deoxyribonucleoside 3'-phosphate
-
size of oligonucleotides 4-9
-
?
5'-hydroxy oligonucleotides + H2O
nucleoside 3'-oligonucleotides + nucleoside 3'-phosphates
-
3',5'-diribonucleoside phosphates and analoga
-
?
5'-hydroxy oligonucleotides + H2O
nucleoside 3'-oligonucleotides + nucleoside 3'-phosphates
-
dependency on type of nucleobase
-
?
5'-hydroxy oligonucleotides + H2O
nucleoside 3'-oligonucleotides + nucleoside 3'-phosphates
-
no hydrolysis of 2'-5' and 5'-5' internucleotide bonds and CpC and CpU-linkages
-
?
5'-hydroxy oligonucleotides + H2O
nucleoside 3'-oligonucleotides + nucleoside 3'-phosphates
-
5'-hydroxy-thymidine oligonucleotides , 5'-hydroxy-adenine oligonucleotides, 5'-hydroxy-2'-deoxycytidine oligonucleotides and various other 5'-hydroxy- and 5'-hydroxy-2'deoxy oligonucleotides like ApUp, d-TpTpC
-
r
5'-hydroxy oligonucleotides + H2O
nucleoside 3'-oligonucleotides + nucleoside 3'-phosphates
-
dependency on type of nucleobase
-
?
bis-(4-nitrophenyl)phosphate + H2O
4-nitrophenyl phosphate + 4-nitrophenol
-
-
-
?
bis-(4-nitrophenyl)phosphate + H2O
4-nitrophenyl phosphate + 4-nitrophenol
-
-
-
?
DNA + H2O
deoxyribonucleoside 3'-phosphates
-
denatured only, no native or double stranded
-
?
DNA + H2O
deoxyribonucleoside 3'-phosphates
-
Ape2 preferentially removes mismatched 3'-nucleotides from DNA
-
-
?
DNA + H2O
deoxyribonucleoside 3'-phosphates
-
Trm2p displays 5' to 3' exonuclease activity on double-strand DNA, and endonuclease activity on single-strand DNA
-
-
?
DNA + H2O
deoxyribonucleoside 3'-phosphates
-
alkali-denaturated DNA, 25 times lower degradation rate with native DNA
-
?
DNA + H2O
deoxyribonucleoside 3'-phosphates
-
5'-OH and 5'-P DNA with chain length of 100-2500
-
?
polyadenylic acid + H2O
5'-hydroxy-adenine oligonucleotides + 3'AMP
-
-
-
r
polyadenylic acid + H2O
5'-hydroxy-adenine oligonucleotides + 3'AMP
-
-
-
?
polynucleotides + H2O
3'-phosphomononucleotides
-
involvement in DNA repair and replication, genetic recombination, elimination of damaged nucleotides
-
?
polynucleotides + H2O
3'-phosphomononucleotides
-
involvement in DNA repair and replication, genetic recombination, elimination of damaged nucleotides
-
?
polynucleotides + H2O
3'-phosphomononucleotides
-
5' -> 3' exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sites
-
-
?
ribonucleotide 3'-alkyl esters + H2O
3'-Ribonucleotides + alkyl alcohol
-
-
-
?
ribonucleotide 3'-alkyl esters + H2O
3'-Ribonucleotides + alkyl alcohol
-
-
-
r
RNA core + H2O
nucleoside 3'-phosphates
-
RNA core are water undializable 3'-phosphoribooligonucleotides obtained by exhaustive digestion of RNA with pancreatic ribonuclease
-
?
thymidine 3'-(2,4-dinitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 2,4-dinitrophenol
-
-
-
?
thymidine 3'-(2,4-dinitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 2,4-dinitrophenol
-
-
-
?
thymidine 3'-(2,4-dinitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 2,4-dinitrophenol
-
-
-
?
thymidine 3'-(2,4-dinitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 2,4-dinitrophenol
-
-
-
?
thymidine 3'-(4-nitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 4-nitrophenol
-
-
-
?
thymidine 3'-(4-nitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 4-nitrophenol
-
-
-
?
thymidine 3'-(4-nitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 4-nitrophenol
-
-
-
?
thymidine 3'-(4-nitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 4-nitrophenol
-
-
-
?
thymidine 3'-(4-nitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 4-nitrophenol
-
-
-
?
thymidine 3'-(4-nitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 4-nitrophenol
-
-
-
?
thymidine 3'-(4-nitrophenyl)phosphate + H2O
thymidine 3'-phosphate + 4-nitrophenol
-
very slow hydrolysis
-
?
tRNA + H2O
tRNA fragments + nucleoside 3'-phosphates
-
5'-phosphoryl terminus hydrolysed by contaminating endonucleases
-
?
tRNA + H2O
tRNA fragments + nucleoside 3'-phosphates
-
phosphorylated or dephosphorylated at 5'-terminus
-
?
tRNA + H2O
tRNA fragments + nucleoside 3'-phosphates
-
from Escherichia coli
-
?
additional information
?
-
-
[RP-but last one-PS]-d[T12] is not hydrolyzed
-
-
?
additional information
?
-
-
NurA may function closely together with SSB in DNA transactions in archaea
-
-
?
additional information
?
-
-
NurA has ssDNA endonuclease, 5'-3' ssDNA exonuclease, and 5'-3' dsDNA exonuclease activities, overview. NurA is closely linked to Mre11 and Rad50 homologues, it interacts with single-stranded DNA-binding protein, StoSSB, from the hyperthermophilic archaeon Sulfolobus tokodaii, determination by pull-down assay using Ni-NTA agarose beads and MALDI-TOF mass spectrometry, and yeast two-hybrid and co-immunoprecipitation analysis, overview. Substrates are 34-bp dsDNA and M13mp18 ssDNA
-
-
?
