3.1.16.1: spleen exonuclease

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For detailed information about spleen exonuclease, go to the full flat file.

Reaction

exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates =

Synonyms

3'-5' exonuclease, 3'-exonuclease, 3'-nucleotide phosphodiesterase, 5' -> 3' exonuclease Xrn2, 5' 3'-exonuclease, 5'-to-3' exonuclease, 5->3 exoribonuclease, Ape2, beef spleen exonuclease, BSEase, BSP, EC 3.1.4.18, exonuclease, FEN-1, flap endonuclease-1, More, NPP1, nucleotide pyrophosphatase/phosphodiesterase 1, phosphodiesterase II, spleen phosphodiesterase, Trm2p, XRNA, XRNB, XRNC, XRND

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.16 Exonucleases that are active with either ribo- or deoxyribonucleic acids and produce 3'-phosphomonoesters

                3.1.16.1 spleen exonuclease

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2	Activating Compound
39	AA Sequence
12	Application
1	CAS Registry Number
6	Cloned(Commentary)
47	Disease/ Diagnostics
14	IC50 Value
44	Inhibitors
1	Ki Value [mM]
12	KM Value [mM]
11	Localization
8	Metals/Ions
12	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
38	Organism
11	pH Optimum
4	pH Range
1	pH Stability
2	Posttranslational Modification
4	Protein Variants
17	Purification (Commentary)
2	Reaction
1	Reaction Type
38	Reference
26	Source Tissue
12	Specific Activity [micromol/min/mg]
3	Storage Stability
74	Substrates and Products (Substrate)
2	Subunits
40	Synonyms
3	Temperature Optimum [°C]
7	Temperature Stability [°C]
3	Turnover Number [1/s]

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Cloned on EC 3.1.16.1 - spleen exonuclease

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

Escherichia coli BL21-Gold DE3 transformed with the plasmid pCBP-TRM2 and pCBP-DTRM2(ctdelta1689-1920nt), also cloned into a GFP vector bearing a GAL1::GFP transcriptional fusion

Saccharomyces cerevisiae

-

681898

FEN-1 cloned into the pCAL-n-FLAG expression vector and expressed in Escherichia coli BLR(DE3)pLysS cells. C-terminal truncation mutant cloned into pET24d(+) and expressed in Escherichia coli BL21(DE3)CodonPlus-RIL

Plasmodium falciparum

Q9GZ01

681823

full-length, sequence verified PCR product cloned into vector pENTR3C, wild type and mutant Ape2 cDNAs in fusion with GST under the control of the Saccharomyces cerevisiae galactose-inducible phosphoglycerate promoter using plasmid pBJ842, resulting in plasmids pIL1047 and pIL1247, respectively, overexpressed in parallel in the protease-deficient BJ5464 yeast strain

Homo sapiens

-

682165

gene nurA, DNA and amino acid sequence determination and analysis, the nurA gene is arranged in an operon-like structure with mre11, rad50, and herA genes, expression with StoSSB in the two-hybrid system in yeast AH109

Sulfurisphaera tokodaii

-

690712

His-tagged XRND protein expressed in Escherichia coli by cloning into pET3A vector

Trypanosoma brucei

-

682836

HIS6 tagged gene subcloned into pET24d(+) and expressed in Escherichia coli BL21(DE3)CodonPlus-RIL

Plasmodium yoelii

Q7RME3

681823