3.1.16.1: spleen exonuclease
This is an abbreviated version!
For detailed information about spleen exonuclease, go to the full flat file.
Word Map on EC 3.1.16.1
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3.1.16.1
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flap
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endonuclease-1
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structure-specific
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okazaki
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nucleases
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micrococcal
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snake
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polynucleotide
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5'-flaps
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32p-postlabeling
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3'-monophosphate
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long-patch
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lagging-strand
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2'-deoxyguanosine
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5'-exonuclease
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exonucleolytic
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dinucleoside
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fen1
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analysis
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internucleotide
- 3.1.16.1
- flap
-
endonuclease-1
-
structure-specific
-
okazaki
- nucleases
-
micrococcal
-
snake
- polynucleotide
-
5'-flaps
-
32p-postlabeling
- 3'-monophosphate
-
long-patch
-
lagging-strand
- 2'-deoxyguanosine
- 5'-exonuclease
-
exonucleolytic
-
dinucleoside
- fen1
- analysis
-
internucleotide
Reaction
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates =
Synonyms
3'-5' exonuclease, 3'-exonuclease, 3'-nucleotide phosphodiesterase, 5' -> 3' exonuclease Xrn2, 5' 3'-exonuclease, 5'-to-3' exonuclease, 5->3 exoribonuclease, Ape2, beef spleen exonuclease, BSEase, BSP, EC 3.1.4.18, exonuclease, FEN-1, flap endonuclease-1, More, NPP1, nucleotide pyrophosphatase/phosphodiesterase 1, phosphodiesterase II, spleen phosphodiesterase, Trm2p, XRNA, XRNB, XRNC, XRND
ECTree
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Engineering
Engineering on EC 3.1.16.1 - spleen exonuclease
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D277A
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mutation of the active-site residue, inactivates 3'5' exonuclease, 3'-phosphodiesterase and AP-endonuclease activities
additional information
C-terminal truncation mutant lacking the terminal 250 amino acids has endonuclease activity that is ca. 130fold greater than full-length FEN-1
additional information
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C-terminal truncation mutant lacking the terminal 250 amino acids has endonuclease activity that is ca. 130fold greater than full-length FEN-1
additional information
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C-terminal truncation mutant lacking the terminal 250 amino acids has endonuclease activity that is ca. 130fold greater than full-length FEN-1
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additional information
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Trm2p(ctdelta76aa), lacks 76 amino acids at the C-terminus, retains nuclease activities but not the methyl transferase activity. Trm2(delta232-1920nt) mutant, contains only the first 231 nucleotides of the TRM2 gene, displays low sensitivity to methyl methane sulfonate and suppresses the methyl methane sulfonate sensitivity of rad52 mutants in trm2(delta232-1920nt)rad52 double mutants. Trm2 exo1 double mutants are synergistically more sensitive to methyl methane sulfonate and ionizing radiation than either of the single mutant. The ku80trm2(deltaD232-1920nt) double mutant displays much higher methyl methane sulfonate sensitivity than either of the single mutant alone. The trm2 (delta232-1920nt) single mutant conferrs only a very slight sensitivity to gamma irradiation, while the trm2(delta232-1920nt)exo1 double mutant exhibits 1.7-2.2fold higher sensitivity relative to trm2(delta232-1920nt) mutant