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3.1.13.4: poly(A)-specific ribonuclease

This is an abbreviated version!
For detailed information about poly(A)-specific ribonuclease, go to the full flat file.

Word Map on EC 3.1.13.4

Reaction

exonucleolytic cleavage of poly(A) to 5'-AMP =

Synonyms

2',3'-exoribonuclease, 3'-exoribonuclease, adenosine-specific exonuclease, AHG2, AtPARN, BTG1-binding factor 1, Caf1, CCR4, CCR4 deadenylase complex, CCR4-associated factor 1, Ccr4-Not complex, CCR4-NOT transcription complex subunit 7, Ccr4p/Pop2p/Notp complex, CNOT6L nuclease, cytoplasmic deadenylase, deadenylase, hPAN, HsPNLDC1, MMAR_3223, More, nuclease, polyadenylate-specific exoribo-, PAB1P-dependent poly(A)-nuclease, PAN, PAN2, PAN3, PARN, PARN-1, PARN-like domain-containing protein 1, PF2046, PNLDC1, poly(A) nuclease, poly(A) nuclease complex, poly(A) ribonuclease, poly(A)-selective ribonuclease, poly(A)-specific 3'-5' ribonuclease, poly(A)-specific 3'-exoribonuclease, poly(A)-specific mRNA exoribonuclease, poly(A)-specific ribonuclease, poly(A)-specific RNase, Pop2p deadenylase, RNase AS, RNase H

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.13 Exoribonucleases producing 5′-phosphomonoesters
                3.1.13.4 poly(A)-specific ribonuclease

Cloned

Cloned on EC 3.1.13.4 - poly(A)-specific ribonuclease

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 genes, expression and sequence analysis, genotyping of plants, expression of His-tagged wild-type and mutant PARN as functional C-terminal maltose-binding protein-fusion protein in Escherichia coli, expression of wild-type GST-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
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AHG2, mutant with Arabidopsis abscisic acid hypersensitivity, expression induced by treatment with Arabidopsis abscisic acid, high salinity and osmotic stress
C-terminal-truncated PARN, expression in Escherichia coli
cloning and expression of full-length PARN and truncated forms p62 and p54
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DNA fragment corresponding to amino acids 443-560 in the human PARN sequence cloned into pCR 2.1TOPO vector and subsequently subcloned into the pET19b vector, recombinant PARN or PARN mutants expressed in Escherichia coli strain BL21(DE3)
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expressed as N-terminal His6-tagged polypeptide in Escherichia coli BL21(DE3)
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in HEK-293T cells
expression in yeast
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expression of enzyme subunits in the two-hybrid system using strain L40, expression of lexA-tagged wild-type and mutant Pab1p, Pan2p, and Pan3p in Escherichia coli, expression of full-length and N-terminal constructs of factor Pbp1p in Escherichia coli, overview
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expression of GFP-tagged wild-type and mutant Pab1p in Xpo1-1 cells at 23°C and at 37°C leading to hyperadenylation of SSA4 mRNA, phenotype or recombinant cells, overview
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expression of the GST-tagged C-terminal 133 amino acids of the PARN-1 open reading, cloned into the pGEX-6P-1 bacterial expression vector, in Escherichia coli strain BL21
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expression of wild-type and mutants of the truncated catalytic domain of human CNOT6L, residues 158-555, in Escherichia coli
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gene PARN, DNA and amino acid sequence determination and analysis, genotyping, quantitative real-time PCR enzyme expression analysis
gene PARN, expression of His-tagged enzyme in Escherichia coli strain Bl21(DE3)
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gene PARN, quantitative real-time PCR enzyme expression analysis
gene PARN, quantitative real-time PCR expression analysis
gene PARN, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene PARN, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta
gene PNLDC1, human PNLDC1-1 and PNLDC1-2 exons arrangement, recombinant expression of EGFP-tagged enzyme in HEK-293 cells, enzyme HsPNLDC1 is produced in two isoforms by alternative splicing of the full-length transcript that contains 19 exons, the peptide residues 1-25 (MDVGADEFEESLPLLQELVQEADFV) of isoform 1 are replaced by the peptide residues 1-14 (MFCTRGLLFFAFLA) in isoform 2 (according to the NCBI annotation), recombinant expression of His-tagged enzyme in Escherichia coli
gene PNLDC1, recombinant expression of EGFP-tagged enzyme in HEK-293 cells, mouse PNLDC1 has one predicted isoform, recombinant expression of His-tagged enzyme in Escherichia coli
His tagged, expression in Escherichia coli BL21 (DE3)
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His-tagged full-length enzyme, comprising residues 1-639, and His-tagged enzyme fragment, PARN(443-560), containing the RNA recognition motif with a 50 amino acids long C-terminal tail, comprising residues 443-560, are expressed in Escherichia coli strain BL21(DE3)
his-tagged recombinant wild type and mutants
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into vector pET28a, expressed in Escherichia coli BL21(DE3)pLys(S) cells
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mutagenized plasmids
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plasmid pET33 containing the human parn gene expressed in Escherichia coli BL21(DE3)
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R3H domain deletion mutants expressed in Escherichia coli
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The DNA fragment encoding the cap-binding domain of PARN (residues 430-516) is amplified from a cDNA clone by PCR and is subcloned into pCR2.1. The cap-binding domain is expressed with a His6-tail, a protease cleavage site, a (Gly-Gly-Ser)2-Gly sequence at the N-terminus and a Ser-Gly-Pro-Ser-Ser-Gly sequence at the C-terminus. Deletion mutants of PARN that contain the cap-binding domain and its N- and/or C-terminal flanking regions, encoding residues 420-506, 420-516, 420-536, 430-506, 430-516 and 430-536, are amplified by PCR and subcloned into pET15b. Selected point mutations are introduced into the cap-binding domain (residues 430-516) using a site-directed mutagenesis kit. The wild type and mutant forms of the cap-binding domain of PARN are overexpressed in Escherichia coli.
The PARN fragment comprising amino acids 445-540 is amplified from a human cDNA library and cloned into the expression vector, the glutathione S-transferase-PARN-RNA-recognition motif fusion protein is expressed in Escherichia coli BL21(DE3). PARN mutants are generated from the wild-type clone (pGEX-6P-1 PARN445-540) using a Site-Directed Mutagenesis Kit. SeMet-containing PARN-RNA-recognition motif is expressed in Escherichia coli.
wild-type and mutant proteins transformed into Escherichia coli Rosetta (DE3) or Rosetta (DE3) pLysS cells