Cloned (Comment) | Organism |
---|---|
expression of the GST-tagged C-terminal 133 amino acids of the PARN-1 open reading, cloned into the pGEX-6P-1 bacterial expression vector, in Escherichia coli strain BL21 | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Cells overexpressing gene PARN-1 have increased deadenylase activity, overexpression of gene PARN-1 leads to PARN-1 affecting the steady-state level and decay rates of at least four different procyclic mRNAs. Depletion of all three PARNs, PARN-1, PARN-2, and PARN-3, using RNA interference in procyclic and bloodstream-form parasites, neither growth rates nor gross morphology are affected in PARN-depleted procyclic forms, and growth rates are only slightly reduced to 90% of control rates in PARN-depleted BF parasites. Following depletion of PARN-1 protein using PARN-1-specific antibodies, RNA-A60 degradation is abolished | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | a poly(A) competitor inhibits RNA-A60 deadenylation by isozyme PARN-1, whereas a poly(C) competitor has no effect on RNA-A60 deadenylation | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Trypanosoma brucei | 5737 | - |
mitochondrion | - |
Trypanosoma brucei | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required | Trypanosoma brucei | |
Mg2+ | required | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
genes PARN-1, PARN-2, and PARN-3 | - |
Trypanosoma brucei Lister 427 | - |
genes PARN-1, PARN-2, and PARN-3 | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged C-terminal 133 amino acids of the PARN-1 from Escherichia coli strain BL21 | Trypanosoma brucei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
29-13 cell | from Procyclic parasites overexpressing PARN-1 | Trypanosoma brucei | - |
additional information | Trypanosomatids possess three different PARN genes, PARN-1, -2, and -3, each of which is expressed at the mRNA level in two life-cycle stages of the human parasite Trypanosoma brucei | Trypanosoma brucei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isozyme PARN-1 degrades RNA-A60. No activity with RNA-A60 with a 22-heteronucleotide sequence added 3' to the poly(A) tail of RNA-A60 as substrate | Trypanosoma brucei | ? | - |
? | |
additional information | isozyme PARN-1 degrades RNA-A60. No activity with RNA-A60 with a 22-heteronucleotide sequence added 3' to the poly(A) tail of RNA-A60 as substrate | Trypanosoma brucei Lister 427 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenosine-specific exonuclease | - |
Trypanosoma brucei |
PARN-1 | - |
Trypanosoma brucei |
poly(A)-specific ribonuclease | - |
Trypanosoma brucei |
poly(A)-specific RNase | - |
Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Trypanosoma brucei |
General Information | Comment | Organism |
---|---|---|
physiological function | PARN-1 is an active deadenylase, PARN-1 is nonessential for parasite viability | Trypanosoma brucei |