Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.13.4 extracted from

  • Liu, W.; Zhang, A.; He, G.; Yan, Y.
    The R3H domain stabilizes poly(A)-specific ribonuclease by stabilizing the RRM domain (2007), Biochem. Biophys. Res. Commun., 360, 846-851.
    View publication on PubMed

Application

Application Comment Organism
additional information R3H domain may stabilize PARN by acting as a protector or intermolecular chaperone of the RRM domain Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
R3H domain deletion mutants expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information removal of the R3H domain (mutant p74DR3H) leads to a dramatic decrease in the thermal stability of PARN, while removal of the RRM domain or both of the two RNA-binding domains only results in a minor decrease in PARN stability against thermal inactivation and denaturation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00036
-
poly(A) RNA full-length 74 kDa PARN Homo sapiens
0.00049
-
poly(A) RNA C-terminal truncated form Homo sapiens
0.00089
-
poly(A) RNA mutant p74deltaR3H Homo sapiens
0.00793
-
poly(A) RNA mutant p54deltaR3H Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
C-terminal truncated form Homo sapiens
74000
-
full-length PARN Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purity above 99% Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) RNA + H2O one RNA-binding domain is required for the substrate binding, but not for the catalysis of PARN Homo sapiens 5'-AMP + ?
-
?

Synonyms

Synonyms Comment Organism
PARN
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
49
-
inactivation of the four PARNs at 49°C, p74 is more stable than p54, p54 is equally stable than mutant p54DR3H, mutant p54DR3H is more stable than mutant p74DR3H Homo sapiens