Application | Comment | Organism |
---|---|---|
additional information | R3H domain may stabilize PARN by acting as a protector or intermolecular chaperone of the RRM domain | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
R3H domain deletion mutants expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | removal of the R3H domain (mutant p74DR3H) leads to a dramatic decrease in the thermal stability of PARN, while removal of the RRM domain or both of the two RNA-binding domains only results in a minor decrease in PARN stability against thermal inactivation and denaturation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00036 | - |
poly(A) RNA | full-length 74 kDa PARN | Homo sapiens | |
0.00049 | - |
poly(A) RNA | C-terminal truncated form | Homo sapiens | |
0.00089 | - |
poly(A) RNA | mutant p74deltaR3H | Homo sapiens | |
0.00793 | - |
poly(A) RNA | mutant p54deltaR3H | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
C-terminal truncated form | Homo sapiens |
74000 | - |
full-length PARN | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purity above 99% | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) RNA + H2O | one RNA-binding domain is required for the substrate binding, but not for the catalysis of PARN | Homo sapiens | 5'-AMP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PARN | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
49 | - |
inactivation of the four PARNs at 49°C, p74 is more stable than p54, p54 is equally stable than mutant p54DR3H, mutant p54DR3H is more stable than mutant p74DR3H | Homo sapiens |