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Literature summary for 3.1.13.4 extracted from

  • Jonstrup, A.T.; Andersen, K.R.; Van, L.B.; Brodersen, D.E.
    The 1.4-.ANG. crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme (2007), Nucleic Acids Res., 35, 3153-3164.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant proteins transformed into Escherichia coli Rosetta (DE3) or Rosetta (DE3) pLysS cells Schizosaccharomyces pombe

Crystallization (Commentary)

Crystallization (Comment) Organism
Pop2p crystallized by the sitting drop vapour diffusion technique, to 1.4 A resolution by X-ray crystallography, crystals belong to the space group P212121, contains a fully conserved DEDDh active site Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
D50A has no activity in the presence of 5mM Mg2+ Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
EDTA activity is completely lost when the ions are removed from the wild-type enzyme by incubation with 10mM Schizosaccharomyces pombe
Zn2+ enzyme is inhibited by high zinc concentration with only two ions and 1mM Zn2+ Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ active site consists of two metal ions (MgA and MgB), residue D50 takes a central position by contacting both metal ions. MgA is also coordinated by E52 and D240, while D171 contacts MgB. Seven water molecules, three associated with MgA and four with MgB, complete the dual octahedral coordination spheres of the two ions Schizosaccharomyces pombe
Mn2+ Mn2+ together with Mg2+ creates a fast and non-specific enzyme Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O74856
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-

Purification (Commentary)

Purification (Comment) Organism
Pop2p purified on Ni2+ column and by gel filtration, to homogeneity Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) RNA + H2O
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Schizosaccharomyces pombe 5'-AMP + ?
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?

Synonyms

Synonyms Comment Organism
Ccr4-Not complex
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Schizosaccharomyces pombe
Pop2p deadenylase
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Schizosaccharomyces pombe