Cloned (Comment) | Organism |
---|---|
wild-type and mutant proteins transformed into Escherichia coli Rosetta (DE3) or Rosetta (DE3) pLysS cells | Schizosaccharomyces pombe |
Crystallization (Comment) | Organism |
---|---|
Pop2p crystallized by the sitting drop vapour diffusion technique, to 1.4 A resolution by X-ray crystallography, crystals belong to the space group P212121, contains a fully conserved DEDDh active site | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
D50A | has no activity in the presence of 5mM Mg2+ | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | activity is completely lost when the ions are removed from the wild-type enzyme by incubation with 10mM | Schizosaccharomyces pombe | |
Zn2+ | enzyme is inhibited by high zinc concentration with only two ions and 1mM Zn2+ | Schizosaccharomyces pombe |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | active site consists of two metal ions (MgA and MgB), residue D50 takes a central position by contacting both metal ions. MgA is also coordinated by E52 and D240, while D171 contacts MgB. Seven water molecules, three associated with MgA and four with MgB, complete the dual octahedral coordination spheres of the two ions | Schizosaccharomyces pombe | |
Mn2+ | Mn2+ together with Mg2+ creates a fast and non-specific enzyme | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | O74856 | - |
- |
Purification (Comment) | Organism |
---|---|
Pop2p purified on Ni2+ column and by gel filtration, to homogeneity | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) RNA + H2O | - |
Schizosaccharomyces pombe | 5'-AMP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ccr4-Not complex | - |
Schizosaccharomyces pombe |
Pop2p deadenylase | - |
Schizosaccharomyces pombe |