3.1.13.4: poly(A)-specific ribonuclease
This is an abbreviated version!
For detailed information about poly(A)-specific ribonuclease, go to the full flat file.
Word Map on EC 3.1.13.4
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3.1.13.4
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deadenylation
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polya-specific
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rna-binding
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decapping
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polyadenylation
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polya-binding
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nocturnin
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au-rich
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mirna-mediated
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telomere
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trim
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argonaute
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eif4e
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pirnas
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pumilio
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tristetraprolin
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cap-binding
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congenita
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exonucleolytic
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p-bodies
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dyskeratosis
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oligoadenylation
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pabps
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diagnostics
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miriscs
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poly-a
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are-binding
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microrna-mediated
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medicine
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eif4e-binding
- 3.1.13.4
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deadenylation
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polya-specific
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rna-binding
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decapping
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polyadenylation
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polya-binding
- nocturnin
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au-rich
-
mirna-mediated
-
telomere
-
trim
-
argonaute
- eif4e
-
pirnas
-
pumilio
- tristetraprolin
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cap-binding
- congenita
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exonucleolytic
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p-bodies
-
dyskeratosis
-
oligoadenylation
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pabps
- diagnostics
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miriscs
- poly-a
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are-binding
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microrna-mediated
- medicine
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eif4e-binding
Reaction
exonucleolytic cleavage of poly(A) to 5'-AMP =
Synonyms
2',3'-exoribonuclease, 3'-exoribonuclease, adenosine-specific exonuclease, AHG2, AtPARN, BTG1-binding factor 1, Caf1, CCR4, CCR4 deadenylase complex, CCR4-associated factor 1, Ccr4-Not complex, CCR4-NOT transcription complex subunit 7, Ccr4p/Pop2p/Notp complex, CNOT6L nuclease, cytoplasmic deadenylase, deadenylase, hPAN, HsPNLDC1, MMAR_3223, More, nuclease, polyadenylate-specific exoribo-, PAB1P-dependent poly(A)-nuclease, PAN, PAN2, PAN3, PARN, PARN-1, PARN-like domain-containing protein 1, PF2046, PNLDC1, poly(A) nuclease, poly(A) nuclease complex, poly(A) ribonuclease, poly(A)-selective ribonuclease, poly(A)-specific 3'-5' ribonuclease, poly(A)-specific 3'-exoribonuclease, poly(A)-specific mRNA exoribonuclease, poly(A)-specific ribonuclease, poly(A)-specific RNase, Pop2p deadenylase, RNase AS, RNase H
ECTree
3.1.13.4 poly(A)-specific ribonucleaseAdvanced search results
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results (Natural Substrates Products
Natural Substrates Products on EC 3.1.13.4 - poly(A)-specific ribonuclease
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NATURAL SUBSTRATE 
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
miR-362-5p + H2O
?
miR-362-5p is defined by DROSHA and DICER to 26 nt in length
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?
mRNA-poly(A) + H2O
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the enzyme is involved in the removal of polyA tails from mRNAs
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-
?
poly(A) RNA + H2O
5'-AMP + ?
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exonucleolytic cleavage of poly(A) in either the single- or double-stranded form (poly(A)*poly(U)) to 5'-AMP (3'-exonucleolytic activity)
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?
poly(A) RNA + H2O
5'-AMP + ?
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does not disintegrate polyribosomes isolated from quail oviduct
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?
additional information
?
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mRNA deadenylation by exoribonucleolytic activity of PARN is essential for embryogenesis and viability, deadenylation of mRNA is often the first and rate-limiting step in mRNA decay, loss of the enzyme affects poly(A) tail length distribution of only a select subset of embryonic transcripts
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?
additional information
?
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mRNA deadenylation by exoribonucleolytic activity of PARN is not essential for embryogenesis and viability
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?
additional information
?
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physiological role of enzyme, recognition mechanisms that target mRNA for degradation
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?
additional information
?
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PARN interacts not only with the 3'-end of the mRNA but also with its 5'-end as PARN contains an RNA-recognition motif domain that specifically binds both the poly(A) tail and the 7-methylguanosine cap. The interaction of PARN with the 5'-cap of mRNAs stimulates the deadenylation activity and enhances the processivity of this reaction
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?
additional information
?
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PARN specifically degrades the mRNA poly(A) tails with a free 3'-hydroxyl group and releases 5'-AMP as the mononucleotide product
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-
?
additional information
?
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Poly(A)-specific ribonuclease is a eukaryotic enzyme that efficiently degrades mRNA poly(A) tails
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-
?
additional information
?
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Poly(A)-specific ribonuclease is a eukaryotic enzyme that efficiently degrades mRNA poly(A) tails
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?
additional information
?
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the nuclease domain of CNOT6L exhibits full Mg2+-dependent deadenylase activity with strict poly(A) RNA substrate specificity. The active site of CNOT6L recognizes the RNA substrate through its negatively charged surface
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?
additional information
?
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enzyme is involved in processing of microRNA. Upon leavage of the 3' arm of the pre-miR-451 precursor hairpin by Argonaute2, the 3' end of the cleaved pre-miR-451 intermediate is then trimmed to the mature length by poly(A)-specific ribonuclease PARN. Trimming requires a 3'-5' exonucleolytic activity, prefers adenosine to uridine and depends on the presence of Mg2+
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?
additional information
?
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Poly(A)-specific ribonuclease (PARN) is a deadenylase that processes mRNAs and non-coding RNA
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?
additional information
?
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Poly(A)-specific ribonuclease (PARN) is a deadenylase that processes mRNAs and non-coding RNA
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?
additional information
?
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shortening of the poly(A) tail is performed by the multi-subunit Ccr4-Not deadenylase, which contains the Caf1 (Pop2) and Ccr4 catalytic components, and poly(A)-specific ribonuclease (PARN)
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?
additional information
?
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shortening of the poly(A) tail is performed by the multi-subunit Ccr4-Not deadenylase, which contains the Caf1 (Pop2) and Ccr4 catalytic components, and poly(A)-specific ribonuclease (PARN)
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?
additional information
?
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shortening of the poly(A) tail is performed by the multi-subunit Ccr4-Not deadenylase, which contains the Caf1 (Pop2) and Ccr4 catalytic components, and poly(A)-specific ribonuclease (PARN)
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?
additional information
?
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PARN is a divalent metal ion-dependent 3'-exonuclease that specifically catalyzes the 3'-5'-degradation of the single-stranded poly(A) tail of mRNA with a free 3'-hydroxyl group
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?
additional information
?
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PARN is a divalent metal ion-dependent 3'-exonuclease that specifically catalyzes the 3'-5'-degradation of the single-stranded poly(A) tail of mRNA with a free 3'-hydroxyl group
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?
additional information
?
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PARN is one of the major mammalian 3'-specific exoribonucleases involved in the degradation of the mRNA poly(A)-tail and it is also involved in the regulation of translation in early embryonic development, it is the key enzyme involved in the deadenylation of mRNA in a cap-dependent manner
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?
additional information
?
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PARN is one of the major mammalian 3'-specific exoribonucleases involved in the degradation of the mRNA poly(A)-tail and it is also involved in the regulation of translation in early embryonic development, it is the key enzyme involved in the deadenylation of mRNA in a cap-dependent manner
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?
additional information
?
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PARN protein levels are not appreciably altered by lipopolysaccharide stimulation in RAW264.7 macrophage cells
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?
additional information
?
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the interaction between PARN and the 7-methylguanosine cap of mRNA plays a key role in stimulating the rate of deadenylation
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?
additional information
?
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the interaction between PARN and the 7-methylguanosine cap of mRNA plays a key role in stimulating the rate of deadenylation
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?
additional information
?
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RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate
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?
additional information
?
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RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate
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?
additional information
?
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RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate
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?
additional information
?
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RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate
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?
additional information
?
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the enzyme plays an important role in the posttranscriptional maturation of mRNA poly(A) tails, factors involved in positive, e.g. Pan3p, and negative, e.g. Pbp1p, regulation, schematic model, overview
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?
additional information
?
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the poly(A)-binding protein, Pab1, and PAN, a poly(A) nuclease complex recruited by Pab1, connect mRNA biogenesis and 3' processing to export, Pab1p is essential, but several bypass suppressors exist, deletion leads to exosome-dependent retention at sites of transcription and inefficient nRNA release, e.g. of SSA4 mRNA, Pab1p and PAN interaction mechanism, overview
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?
additional information
?
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mRNA deadenylation by exoribonucleolytic activity of PARN is not essential for embryogenesis and viability
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?
