Activating Compound | Comment | Organism | Structure |
---|---|---|---|
K+ | allosteric activator of PARN and its truncated forms, optimal concentration is around 100 mM, with the increase of the K+ concentration, the enzyme reaches its Vmax at a much lower substrate concentration | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cloning and expression of full-length PARN and truncated forms p62 and p54 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cap analogue 7-methylguanosine(5')ppp(5')G | allosteric inhibitor of PARN in the presence of a physiological K+ concentration | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | the binding of K+ to the entire RNA-recognition motif domain, which plays a central role in the allosteric communications of PARN regulation, leads to an increase of substrate-binding affinity but a decrease in the cooperativity of the substrate-binding site, while the binding of the cap analogue decreases both the catalytic efficiency and the substrate-binding affinity | Homo sapiens | |
Mg2+ | essential for PARN activity and stability | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
PARN (p54), residues 1-470 | Homo sapiens |
62000 | - |
PARN (p62), residues 1-540 | Homo sapiens |
74000 | - |
full-length PARN (p74) | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | PARN specifically degrades the mRNA poly(A) tails with a free 3'-hydroxyl group and releases 5'-AMP as the mononucleotide product | ? | - |
? | |
poly(A) RNA + H2O | Homo sapiens | deadenylation of mRNA | 5'-AMP + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of full-length PARN and truncated forms p62 and p54 | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Vmax value is 0.30 micromol/min/micromol for the full-length enzyme at 25 mM K+ | Homo sapiens |
additional information | - |
Vmax value is 0.36 micromol/min/micromol for the full-length enzyme at 50 mM K+ | Homo sapiens |
additional information | - |
Vmax value is 0.42 micromol/min/micromol for the full-length enzyme at 100 mM K+ | Homo sapiens |
additional information | - |
Vmax value is 0.42 micromol/min/micromol for the full-length enzyme at 200 mM K+ | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PARN specifically degrades the mRNA poly(A) tails with a free 3'-hydroxyl group and releases 5'-AMP as the mononucleotide product | Homo sapiens | ? | - |
? | |
poly(A) RNA + H2O | deadenylation of mRNA | Homo sapiens | 5'-AMP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | multi-domain enzyme | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PARN | - |
Homo sapiens |
poly(A)-specific ribonuclease | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |