1.1.1.146: 11beta-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 11beta-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

an 11beta-hydroxysteroid
+
NADP+
=
an 11-oxosteroid
+
NADPH
+
H+

Synonyms

11 beta-hydroxysteroid dehydrogenase type 1, 11-beta-HSD1, 11-beta-HSD1 (oxidase) NADP+, 11-beta-HSD2, 11-beta-hydroxysteroid dehydrogenase type1, 11-DH, 11-DH2, 11-oxoreductase, 11beta hydroxysteroid dehydrogenase type 1, 11beta-HSD, 11beta-HSD 1, 11beta-HSD type 1, 11beta-HSD1, 11beta-HSD1A, 11beta-HSD2, 11beta-hydroxy steroid dehydrogenase, 11beta-hydroxysteroid dehydrogenase, 11beta-hydroxysteroid dehydrogenase 1, 11beta-hydroxysteroid dehydrogenase isoforms 1, 11beta-hydroxysteroid dehydrogenase type 1, 11beta-hydroxysteroid dehydrogenase type 2, 11beta-hydroxysteroid dehydrogenase type I, 11beta-hydroxysteroid dehydrogenase type-1, 11beta-hydroxysteroid dehydrogenase-1, 11beta-hydroxysteroid dehydrogenases type 1, 11beta-hydroxysteroid-dehydrogenase type I, 11betaHSD1, 11HSD1, beta-hydroxysteroid dehydrogenase, corticosteroid 11-reductase, corticosteroid 11beta-dehydrogenase, dehydrogenase, 11beta-hydroxy steroid, HSD1, HSD11B, Hsd11B2, isozyme 11beta-HSD1, More, NAD-dependent 11-beta-hydroxysteroid dehydrogenase, NADP(+)-dependent 11beta-dehydrogenase, NADP+-dependent 11beta-hydroxysteroid dehydrogenase 1, type 1 11beta-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.146 11beta-hydroxysteroid dehydrogenase

Purification

Purification on EC 1.1.1.146 - 11beta-hydroxysteroid dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity partitioning of His-tagged 11beta-HSD1 in polymer/detergent aqueous two-phase system; recombinant His6-tagged isozyme 11beta-HSD1 from membrane by microsome preparation, in situ solubilization, partitioning and batch metal affinity adsorption in aqueous two-phase systems, optimization of solubilization using different detergents at 1% w/v, overview
-
native enzyme from liver microsomes partially by microsome preparation
native enzyme from liver microsomes to homogeneity
-
near homogeneity, recombinant enzyme
-
octyl-Sepharose, Mono Q-sepharose, Red Sepharose A
Octyl-Sepharose, Mono Q-Sepharose, Red-Sepharose
-
purified in the presence of CHAPS by Ni+-NTA column chromatography and Superdex 200 gel filtration
-
recombinant His-tagged-11beta-HSD1 expressed in Escherichia coli
-
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant His6-tagged isozyme 11beta-HSD1 from Escherichia coli strain BL21(DE3) by metal affinity chromatography
recombinant isozyme 11beta-HSD1 catalytic domain mutant C272S from Escherichia coli strain DH10b-Tir by affinity chromatography
-
solubilization, partially purifed by gel filtration, 73fold
-
solubilized microsomes, octyl-Sepharose, Mono Q-Sepharose, Red Sepharose A
-
to homogeneity, affinity chromatography
-
to homogeneity, chromatography techniques
-
to homogeneity, recombinant catalytic domain
-
to homogeneity, recombinant enzyme
-