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Literature summary for 1.1.1.146 extracted from

  • Walker, E.A.; Clarks, A.M.; Hewison, M.; Ride, J.P.; Steward, P.M.
    Functional expression, characterization, and purification of the catalytic domain of human 11-beta-hydroxysteroid dehydrogenase type 1 (2001), J. Biol. Chem., 276, 21343-21350.
    View publication on PubMed
Search for more literature for 1.1.1.146

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, catalytic domain Homo sapiens

Protein Variants

Protein Variants Comment Organism
C272S ability to form disulfide bonds abolished, but no change in activity or Km Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione i.e. cortisol Homo sapiens
0.0014
-
 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione recombinant catalytic domain Homo sapiens
0.0095
-
 17,21-dihydroxy-pregn-4-ene-3,11,20-trione i.e. cortisone Homo sapiens
0.0095
-
 17,21-dihydroxy-pregn-4-ene-3,11,20-trione recombinant catalytic domain Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH Homo sapiens implicated in the pathogenesis of insulin resistance and obesity 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol r
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH Homo sapiens i.e. cortisone 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isoenzyme type 1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant catalytic domain Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH implicated in the pathogenesis of insulin resistance and obesity Homo sapiens 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol r
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH i.e. cortisone Homo sapiens 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol r

Synonyms

Synonyms Comment Organism
11beta-HSD 2 isoforms: 11beta-HSD1 and 11beta-HSD2 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Homo sapiens
NADPH
-
 Homo sapiens