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Literature summary for 1.1.1.146 extracted from

  • Lakshmi, V.; Monder, C.
    Purification and characterization of the corticosteroid 11beta-dehydrogenase component of the rat liver 11beta-hydroxysteroid dehydrogenase complex (1988), Endocrinology, 123, 2390-2398.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00183
-
 11beta,21-dihydroxypregn-4-en-3,20-dione
-
 Rattus sp.
0.0173
-
 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione i.e. cortisol Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ Rattus sp. i.e. cortisol 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH i.e. cortisone ir
21-hydroxy-pregn-4-en-3,11,20-trione + NADPH Rattus sp. 10times higher activity than for cortisol 11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ i.e. corticosterone ir

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, affinity chromatography Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol Rattus sp. 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH i.e. cortisone ir
21-hydroxy-pregn-4-en-3,11,20-trione + NADPH 10times higher activity than for cortisol Rattus sp. 11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ i.e. corticosterone ir

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Rattus sp.