1.1.1.103: L-threonine 3-dehydrogenase

This is an abbreviated version, for detailed information about L-threonine 3-dehydrogenase, go to the full flat file.

Reaction

L-threonine
+
NAD+
=
L-2-amino-3-oxobutanoate
+
NADH
+
H+

Synonyms

CLOST_1621, L-ThrDH, L-threonine dehydrogenase, More, orf382, TDG, TDH, Thr dehydrogenase, ThrDH, threonine 3-dehydrogenase, threonine dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.103 L-threonine 3-dehydrogenase

Purification

Purification on EC 1.1.1.103 - L-threonine 3-dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acid precipitation, ammonium sulfate, Sephadex G-100, Sephadex G-200
-
ammonium sulfate, DEAE-cellulose, 7fold purification
-
by heat treatment (about 80% pure recombinant TDH protein) and gel filtration
-
by sonication, centrifugation and on a Ni-NTA resin column
-
by sonication, centrifugation, heating of the soluble fraction, anion-exchange and hydrophobic interaction chromatography
-
by sonication, heating of the cell lysate, anion exchange and hydrophobic interaction chromatography
-
chromatography on Q-Sepharose and Reactive Green 19-agarose
DEAE Sepharose FF, Affi-Gel blue, Sephacryl S-200, Matrix Gel red A, Matrix gel Green A
-
DEAE-cellulose, ammonium sulfate, DEAE-cellulose, Blue dextran-Sepharose, Sephadex G-200, amylamine sepharose, Sephadex G-200
-
DEAE-cellulose, hydrophobic interaction chromatography, Affi-Gel Blue, Matrex Red A
-
hydroxyapatite
-
MnCl2, ammonium sulfate, calcium phosphate gel, protamine sulfate, DEAE-cellulose, 7fold purification
-
Ni-chelating column chromatography and Superdex 200 gel filtration
-
Ni-NTA agarose column chromatography
Ni2+-Sepharose column chromatography and Superdex 75 gel filtration
-
protamine sulfate, Sephadex G-200, DEAE-cellulose
-
recombinant enzyme 48fold from Escherichia coli to homogeneity by heat treatment and anion exchange chromatography
-
recombinant enzyme from Escherichia coli
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 85C for 30 min, centrifugation, ion exchange chromatography, and gel filtration
-
recombinant His-tagged enzyme 4.2fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, native enzyme 75.4fold by anion exchange and hydrophobic interaction chromatography, dialysis, hydroxyapatite chromatography, followed by gel filtration
recombinant His-tagged enzyme from Escherichia coli strain TOP10 by nickel affinity chromatography
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heat treatment at 85C for 30 min, anion exchange and hydrophobic interaction chromatography
-
treatment of cell extract at 60C, DEAE-Sephadex, Mn2+ activation, Blue 2-Sepharose CL-6B, C38D mutant was purified without Mn2+ activation
-