1.1.1.103: L-threonine 3-dehydrogenase

This is an abbreviated version, for detailed information about L-threonine 3-dehydrogenase, go to the full flat file.

Reaction

L-threonine
+
NAD+
=
L-2-amino-3-oxobutanoate
+
NADH
+
H+

Synonyms

CLOST_1621, L-ThrDH, L-threonine dehydrogenase, More, orf382, TDG, TDH, Thr dehydrogenase, ThrDH, threonine 3-dehydrogenase, threonine dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.103 L-threonine 3-dehydrogenase

Specific Activity

Specific Activity on EC 1.1.1.103 - L-threonine 3-dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.029
-
partially purified enzyme
0.032
-
enzyme activity in mitochondrial extracts prepared from fresh mitochondria
0.055
-
enzyme activity in mitochondrial extracts
0.062
-
enzyme activity in mitochondrial extracts after freezing the mitochondria for 2 weeks at -20°C
0.1
-
C38D mutant, incubated with 1 mM Mn2+ 15 min before assay
0.2
-
C38D mutant
0.6
-
C38D mutant, incubated with 0.07 or 5 mM Cd2+ 15 min before assay
1.2
-
C38D mutant, incubated with 5 mM Co2+ 15 min before assay
2
-
C38D mutant, incubated with 5 mM Zn2+ and 1 mM Mn2+ 15 min before assay
3.43
-
purified recombinant enzyme
8
-
fully demetalized enzyme
8.45
-
-
10.2
-
C38D mutant, incubated with 5 mM Zn2+ and 0.07 mM Cd2+ 15 min before assay
11.4
-
C38D mutant, incubated with 5 mM Zn2+ 15 min before assay
28.3
-
metal-ion free enzyme
42.2
purified native enzyme, pH 10.0, 30°C
65
purified recombinant enzyme, pH 10.0, 30°C
91.4
pH 9.0, 10°C
156
pH 9.0, 20°C
234
L-threonine, pH 9.0, 37°C
243
-
enzyme activated with 0.07 mM Cd2+
284
pH 9.0, 30°C
380
-
-
611
pH 9.0, 50°C
685
-
enzyme activated with 0.25 mM Mn2+
722
pH 9.0, 40°C
18920
-
-
additional information