Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.9 mM, 61% inhibiton | Acetoanaerobium sticklandii | |
Cu2+ | 0.9 mM, 54% inhibiton | Acetoanaerobium sticklandii | |
additional information | no inhibition by Ni2+, Ca2+, Mg2+, Mn2+ | Acetoanaerobium sticklandii | |
Zn2+ | 0.9 mM, 23% inhibiton | Acetoanaerobium sticklandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NAD+ | pH 9.0, 37°C | Acetoanaerobium sticklandii | |
18 | - |
L-threonine | pH 9.0, 37°C | Acetoanaerobium sticklandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration, monomeric enzyme | Acetoanaerobium sticklandii |
80000 | - |
gel filtration, dimeric enzyme | Acetoanaerobium sticklandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Acetoanaerobium sticklandii | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
L-threonine + NAD+ | Acetoanaerobium sticklandii DSM 519 | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | E3PS87 | - |
- |
Acetoanaerobium sticklandii DSM 519 | E3PS87 | - |
- |
Purification (Comment) | Organism |
---|---|
chromatography on Q-Sepharose and Reactive Green 19-agarose | Acetoanaerobium sticklandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
234 | - |
L-threonine, pH 9.0, 37°C | Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoin + NAD+ | 1% of the activity compared to L-threonine | Acetoanaerobium sticklandii | butan-2,3-dione + NADH + H+ | - |
? | |
acetoin + NAD+ | 1% of the activity compared to L-threonine | Acetoanaerobium sticklandii DSM 519 | butan-2,3-dione + NADH + H+ | - |
? | |
DL-3-hydroxynorvaline + NAD+ | 20% of the activity compared to L-threonine | Acetoanaerobium sticklandii | 3-oxonorvaline + NADH + H+ | - |
? | |
DL-3-hydroxynorvaline + NAD+ | 20% of the activity compared to L-threonine | Acetoanaerobium sticklandii DSM 519 | 3-oxonorvaline + NADH + H+ | - |
? | |
L-threonine + NAD+ | - |
Acetoanaerobium sticklandii | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
L-threonine + NAD+ | - |
Acetoanaerobium sticklandii DSM 519 | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
additional information | no activity with L-theronine methyl ester, L-threonine amide, DL-threonine hydroxamate, L-homoserine, (R)-1-amino-2-propanol, L-malate, (R)-3-hydroxybutyrate, DL-threo-phenylserine, L-serine | Acetoanaerobium sticklandii | ? | - |
? | |
additional information | no activity with L-theronine methyl ester, L-threonine amide, DL-threonine hydroxamate, L-homoserine, (R)-1-amino-2-propanol, L-malate, (R)-3-hydroxybutyrate, DL-threo-phenylserine, L-serine | Acetoanaerobium sticklandii DSM 519 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35000, SDS-PAGE, acitve in monomeric and in dimeric enzyme form | Acetoanaerobium sticklandii |
Synonyms | Comment | Organism |
---|---|---|
CLOST_1621 | locus name | Acetoanaerobium sticklandii |
threonine dehydrogenase | - |
Acetoanaerobium sticklandii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Acetoanaerobium sticklandii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NADP+, thio-NAD+, or 3-acetylpyridine adenine dinucleotide cannot substitute for NAD+ | Acetoanaerobium sticklandii |