1.1.1.103: L-threonine 3-dehydrogenase

This is an abbreviated version, for detailed information about L-threonine 3-dehydrogenase, go to the full flat file.

Reaction

L-threonine
+
NAD+
=
L-2-amino-3-oxobutanoate
+
NADH
+
H+

Synonyms

CLOST_1621, L-ThrDH, L-threonine dehydrogenase, More, orf382, TDG, TDH, Thr dehydrogenase, ThrDH, threonine 3-dehydrogenase, threonine dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.103 L-threonine 3-dehydrogenase

Inhibitors

Inhibitors on EC 1.1.1.103 - L-threonine 3-dehydrogenase

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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
1.26 mM, 41% inhibition after 1 h, 82% inhibition after 2 h, no change in remaining activity after removal of 1,10-phenanthroline
4-chloromercuribenzonic acid
94% inhibition at 10 mM
5,5'-dithiobis-(2-nitrobenzoic acid)
-
0.25 mM, 90% inhibition, 67% activity is recovered after incubation with 1 mM, 2-mercaptoethanol or dithieothreitol for 15 min
adenosine-5'-diphosphoribose
aminoacetone
-
uncompetitive inhibition vs. NAD+ or L-threonine
Be2+
-
3.2 mM, 20-50% inhibition
calcium pantothenate
slight inhibition
Cd2+
-
0.05 and 1.0 mM, 90% inhibition
Co2+
0.9 mM, 61% inhibiton
CuCl2
1 mM, 100% inhibition
dipicolinic acid
-
40 mM, 99% inhibition after 1 h, complete loss of enzyme-bound Zn2+
FeCl2
32% inhibition at 1 mM after 60 min
FeCl3
73% inhibition at 1 mM after 60 min
HCO3-
-
noncompetitive inhibition vs. NAD+ or L-threonine
HgCl2
iodoacetamide
iodoacetate
iodoacetic acid
52% inhibition at 10 mM
Iodosobenzoic acid
-
0.3 mM, 17% inhibition
K3[Fe(CN)6]
25% inhibition at 10 mM
L-2-amino-3-oxobutyrate
-
competitive product inhibition by the unstable L-2-amino-3-oxobutyrate only in presence of NADH, which stabilizes
methyl p-nitrobenzenesulfonate
-
2 mM, 40% inhibition within 80 min, 65% protection with 250 mM L-threonine, 64% with 250 mM L-threonine methyl ester, 58% with 250 mM L-threonine amide
methylglyoxal
-
1.0 mM, 42% inhibition, 2 mM, 63% inhibition, methylglyoxal binds at an allosteric site of the enzyme
methylmethanethiosulfonate
-
0.4 mM, 350fold molar excess over enzyme sulfhydryl groups leads to complete inactivation
monoiodoacetate
10 mM, 100% inhibition
N-ethylmaleimide
NAD+
-
competitive inhibition of L-2-amino-3-oxobutanoate reduction
NaN3
88% inhibition at 10 mM
p-chloromercuribenzoate
-
complete inhibition
p-chloromercuribenzoic acid
10 mM, 44% inhibition
p-mercuribenzoate
phenazinemethosulfate
complete inhibition
phenylmethanesulfonyl fluoride
63% inhibition at 10 mM
pyruvate
SnCl2
16% inhibition at 1 mM after 60 min
thionitrobenzoate
-
40fold molar excess, gradual 99% loss of enzyme activity
ZnCl2
1 mM, 72% inhibition
additional information
-