BRENDA - Enzyme Database show
show all sequences of 1.1.1.103

Novel psychrophilic and thermolabile L-threonine dehydrogenase from psychrophilic Cytophaga sp. strain KUC-1

Kazuoka, T.; Takigawa, S.; Arakawa, N.; Hizukuri, Y.; Muraoka, I.; Oikawa, T.; Soda, K.; J. Bacteriol. 185, 4483-4489 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Cytophaga sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
adenosine-5'-diphosphoribose
-
Cytophaga sp.
CuCl2
1 mM, 100% inhibition
Cytophaga sp.
HgCl2
10 mM, 100% inhibition
Cytophaga sp.
monoiodoacetate
10 mM, 100% inhibition
Cytophaga sp.
N-ethylmaleimide
10 mM, 48% inhibition
Cytophaga sp.
NADH
competitive to NAD+, noncompetitive to L-threonine
Cytophaga sp.
p-chloromercuribenzoic acid
10 mM, 44% inhibition
Cytophaga sp.
pyruvate
competitive to L-threonine
Cytophaga sp.
ZnCl2
1 mM, 72% inhibition
Cytophaga sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.56
-
L-threonine
pH 9.0, 20C
Cytophaga sp.
3.81
-
L-threonine
pH 9.0, 10C
Cytophaga sp.
5.96
-
L-threonine
pH 9.0, 30C
Cytophaga sp.
11.2
-
L-threonine
pH 9.0, 40C
Cytophaga sp.
19.5
-
L-threonine
pH 9.0, 50C
Cytophaga sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
4 * 35000, SDS-PAGE
Cytophaga sp.
139000
-
gel filtration
Cytophaga sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cytophaga sp.
Q8KZM4
strain KUC-1
-
Cytophaga sp. KUC-1
Q8KZM4
strain KUC-1
-
Purification (Commentary)
Commentary
Organism
-
Cytophaga sp.
Reaction
Reaction
Commentary
Organism
L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH + H+
ordered bi-bi mechanism, NAD+ binds prior to L-threonine
Cytophaga sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
91.4
-
pH 9.0, 10C
Cytophaga sp.
156
-
pH 9.0, 20C
Cytophaga sp.
284
-
pH 9.0, 30C
Cytophaga sp.
611
-
pH 9.0, 50C
Cytophaga sp.
722
-
pH 9.0, 40C
Cytophaga sp.
Storage Stability
Storage Stability
Organism
-20C, 10 mM potassium phosphate, pH 7.0, 30% sucrose, 0.1 mM NAD+, 5 mM L-threonine, several months, no loss of activity
Cytophaga sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DL-threo-3-hydroxynorvaline + NAD+
31% the rate of L-threonine
655857
Cytophaga sp.
? + NADH
-
-
-
?
DL-threo-3-hydroxynorvaline + NAD+
31% the rate of L-threonine
655857
Cytophaga sp. KUC-1
? + NADH
-
-
-
?
L-threonine + 3-acetyl-pyridine adenine dinucleotide
60.6% the rate of NAD+
655857
Cytophaga sp.
L-2-amino-3-oxobutanoate + ?
-
-
-
?
L-threonine + 3-acetyl-pyridine adenine dinucleotide
60.6% the rate of NAD+
655857
Cytophaga sp. KUC-1
L-2-amino-3-oxobutanoate + ?
-
-
-
?
L-threonine + 3-pyridinealdehyde adenine dinucleotide
7.2% the rate of NAD+
655857
Cytophaga sp.
L-2-amino-3-oxobutanoate + ?
-
-
-
?
L-threonine + 3-pyridinealdehyde adenine dinucleotide
7.2% the rate of NAD+
655857
Cytophaga sp. KUC-1
L-2-amino-3-oxobutanoate + ?
-
-
-
?
L-threonine + NAD+
-
655857
Cytophaga sp.
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
-
-
?
L-threonine + NAD+
-
655857
Cytophaga sp. KUC-1
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
-
-
?
L-threonine + nicotinamide guanine dinucleotide
5.1% the rate of NAD+
655857
Cytophaga sp.
L-2-amino-3-oxobutanoate + ?
-
-
-
?
L-threonine + thionicotinamide-NAD+
5.1% the rate of NAD+
655857
Cytophaga sp.
L-2-amino-3-oxobutanoate + ?
-
-
-
?
additional information
specific for L-form of threonine, no substrate: NADP, nicotinic acid adenine dinucleotide, alpha-NAD, nicotinamide hypoxanthine dinucleotide
655857
Cytophaga sp.
?
-
-
-
-
additional information
specific for L-form of threonine, no substrate: NADP, nicotinic acid adenine dinucleotide, alpha-NAD, nicotinamide hypoxanthine dinucleotide
655857
Cytophaga sp. KUC-1
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 35000, SDS-PAGE
Cytophaga sp.
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Cytophaga sp.
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
10
30
60 min, more than 90% residual activity
Cytophaga sp.
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
half-life 160 min
Cytophaga sp.
45
-
half-life 14 min
Cytophaga sp.
50
-
rapid inactivation
Cytophaga sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Cytophaga sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8.5
-
74% of maximum activity
Cytophaga sp.
10.5
-
31% of maximum activity
Cytophaga sp.
Cofactor
Cofactor
Commentary
Organism
Structure
3-acetyl-pyridine adenine dinucleotide
-
Cytophaga sp.
nicotinamide guanine dinucleotide
-
Cytophaga sp.
thionicotinamide-NAD+
-
Cytophaga sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.37
-
adenosine-5'-diphosphoribose
substrate NAD+, pH 9.0, 30C
Cytophaga sp.
1.42
-
adenosine-5'-diphosphoribose
substrate L-threonine, pH 9.0, 30C
Cytophaga sp.
23.2
-
pyruvate
substrate L-threonine, pH 9.0, 30C
Cytophaga sp.
50.7
-
pyruvate
substrate NAD+, pH 9.0, 30C
Cytophaga sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Cytophaga sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
3-acetyl-pyridine adenine dinucleotide
-
Cytophaga sp.
nicotinamide guanine dinucleotide
-
Cytophaga sp.
thionicotinamide-NAD+
-
Cytophaga sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
adenosine-5'-diphosphoribose
-
Cytophaga sp.
CuCl2
1 mM, 100% inhibition
Cytophaga sp.
HgCl2
10 mM, 100% inhibition
Cytophaga sp.
monoiodoacetate
10 mM, 100% inhibition
Cytophaga sp.
N-ethylmaleimide
10 mM, 48% inhibition
Cytophaga sp.
NADH
competitive to NAD+, noncompetitive to L-threonine
Cytophaga sp.
p-chloromercuribenzoic acid
10 mM, 44% inhibition
Cytophaga sp.
pyruvate
competitive to L-threonine
Cytophaga sp.
ZnCl2
1 mM, 72% inhibition
Cytophaga sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.37
-
adenosine-5'-diphosphoribose
substrate NAD+, pH 9.0, 30C
Cytophaga sp.
1.42
-
adenosine-5'-diphosphoribose
substrate L-threonine, pH 9.0, 30C
Cytophaga sp.
23.2
-
pyruvate
substrate L-threonine, pH 9.0, 30C
Cytophaga sp.
50.7
-
pyruvate
substrate NAD+, pH 9.0, 30C
Cytophaga sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.56
-
L-threonine
pH 9.0, 20C
Cytophaga sp.
3.81
-
L-threonine
pH 9.0, 10C
Cytophaga sp.
5.96
-
L-threonine
pH 9.0, 30C
Cytophaga sp.
11.2
-
L-threonine
pH 9.0, 40C
Cytophaga sp.
19.5
-
L-threonine
pH 9.0, 50C
Cytophaga sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
4 * 35000, SDS-PAGE
Cytophaga sp.
139000
-
gel filtration
Cytophaga sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Cytophaga sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
91.4
-
pH 9.0, 10C
Cytophaga sp.
156
-
pH 9.0, 20C
Cytophaga sp.
284
-
pH 9.0, 30C
Cytophaga sp.
611
-
pH 9.0, 50C
Cytophaga sp.
722
-
pH 9.0, 40C
Cytophaga sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 10 mM potassium phosphate, pH 7.0, 30% sucrose, 0.1 mM NAD+, 5 mM L-threonine, several months, no loss of activity
Cytophaga sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DL-threo-3-hydroxynorvaline + NAD+
31% the rate of L-threonine
655857
Cytophaga sp.
? + NADH
-
-
-
?
DL-threo-3-hydroxynorvaline + NAD+
31% the rate of L-threonine
655857
Cytophaga sp. KUC-1
? + NADH
-
-
-
?
L-threonine + 3-acetyl-pyridine adenine dinucleotide
60.6% the rate of NAD+
655857
Cytophaga sp.
L-2-amino-3-oxobutanoate + ?
-
-
-
?
L-threonine + 3-acetyl-pyridine adenine dinucleotide
60.6% the rate of NAD+
655857
Cytophaga sp. KUC-1
L-2-amino-3-oxobutanoate + ?
-
-
-
?
L-threonine + 3-pyridinealdehyde adenine dinucleotide
7.2% the rate of NAD+
655857
Cytophaga sp.
L-2-amino-3-oxobutanoate + ?
-
-
-
?
L-threonine + 3-pyridinealdehyde adenine dinucleotide
7.2% the rate of NAD+
655857
Cytophaga sp. KUC-1
L-2-amino-3-oxobutanoate + ?
-
-
-
?
L-threonine + NAD+
-
655857
Cytophaga sp.
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
-
-
?
L-threonine + NAD+
-
655857
Cytophaga sp. KUC-1
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
-
-
?
L-threonine + nicotinamide guanine dinucleotide
5.1% the rate of NAD+
655857
Cytophaga sp.
L-2-amino-3-oxobutanoate + ?
-
-
-
?
L-threonine + thionicotinamide-NAD+
5.1% the rate of NAD+
655857
Cytophaga sp.
L-2-amino-3-oxobutanoate + ?
-
-
-
?
additional information
specific for L-form of threonine, no substrate: NADP, nicotinic acid adenine dinucleotide, alpha-NAD, nicotinamide hypoxanthine dinucleotide
655857
Cytophaga sp.
?
-
-
-
-
additional information
specific for L-form of threonine, no substrate: NADP, nicotinic acid adenine dinucleotide, alpha-NAD, nicotinamide hypoxanthine dinucleotide
655857
Cytophaga sp. KUC-1
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 35000, SDS-PAGE
Cytophaga sp.
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Cytophaga sp.
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
10
30
60 min, more than 90% residual activity
Cytophaga sp.
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
half-life 160 min
Cytophaga sp.
45
-
half-life 14 min
Cytophaga sp.
50
-
rapid inactivation
Cytophaga sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Cytophaga sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8.5
-
74% of maximum activity
Cytophaga sp.
10.5
-
31% of maximum activity
Cytophaga sp.
Other publictions for EC 1.1.1.103
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738933
He
Structural insights on mouse L ...
Mus musculus
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192
510-518
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1
1
5
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4
1
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5
5
737579
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Effects of dietary levels of g ...
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27
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1
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1
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738606
Nakano
Binding of NAD+ and L-threonin ...
Cupriavidus necator
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289
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1
1
3
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5
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3
3
738860
Ma
Identification and characteriz ...
Escherichia coli
J. Microbiol. Biotechnol.
24
748-755
2014
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1
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3
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4
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1
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1
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11
1
1
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1
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1
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-
723777
Han
Regulation of L-threonine dehy ...
Mus musculus
Stem Cells
31
953-965
2013
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2
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722747
Yoneda
Crystal structure of binary an ...
Thermoplasma volcanium
J. Biol. Chem.
287
12966-12974
2012
1
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1
1
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2
2
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3
1
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5
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1
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1
1
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1
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1
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1
1
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2
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2
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3
1
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1
1
1
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1
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1
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1
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1
1
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-
721272
Ueatrongchit
Highly selective L-threonine 3 ...
Cupriavidus necator, Cupriavidus necator NBRC 102504
Anal. Biochem.
410
44-56
2011
2
-
1
-
-
-
14
2
-
13
2
2
-
8
-
-
1
-
-
-
2
-
6
1
1
1
1
2
1
1
1
1
-
-
-
2
-
1
1
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-
-
-
14
-
2
-
13
2
2
-
-
-
1
-
-
2
-
6
1
1
1
1
2
1
1
1
-
-
2
2
-
-
-
698526
Bao
Biochemical characteristics an ...
Streptomyces sp. 139
J. Appl. Microbiol.
106
1140-1146
2009
-
-
1
-
1
-
-
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3
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1
-
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1
2
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1
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1
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1
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1
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1
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1
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1
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1
2
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1
-
1
-
1
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1
-
-
-
-
-
-
-
698673
Bashir
Highly thermostable L-threonin ...
Thermococcus kodakarensis KOD1
J. Biochem.
146
95-102
2009
-
-
1
-
-
-
1
2
-
7
2
-
-
5
-
-
1
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-
-
-
-
2
-
1
-
1
-
1
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1
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1
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2
-
7
2
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1
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
699854
Bowyer
Structure and function of the ...
Thermococcus kodakarensis
J. Struct. Biol.
168
294-304
2009
-
-
1
1
-
-
-
-
-
1
1
-
-
3
-
-
1
-
-
-
-
-
1
1
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Crystallization and preliminar ...
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Shimizu
L-Threonine dehydrogenase from ...
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10
1
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Higashi
Kinetic study of thermostable ...
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4
1
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2
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1
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1
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654175
Akagi
Threonine metabolism in Japane ...
Coturnix japonica, Rattus norvegicus
Amino Acids
26
235-242
2004
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Kazuoka
Novel psychrophilic and thermo ...
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4483-4489
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9
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12
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655125
Edgar
Molecular cloning and tissue d ...
Mus musculus, Sus scrofa
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655131
Edgar
The human L-threonine 3-dehydr ...
Homo sapiens
BMC Genet.
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2002
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285701
Yuan
Characterization of hepatic L- ...
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Comp. Biochem. Physiol. B
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2001
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285702
Johnson
Investigation of a catalytic z ...
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1998
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Identification of a second act ...
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Functional analysis of E. coli ...
Escherichia coli K-12
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1995
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Purification and characterizat ...
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Kao
Purification and structural ch ...
Sus scrofa
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423-431
1994
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Threonine formation via the co ...
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Pagani
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Mitochondrial L-threonine dehy ...
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Pagani
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Rat liver L-threonine dehydrog ...
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Epperly
L-Threonine dehydrogenase from ...
Escherichia coli K-12
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266
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Craig
The sulfhydryl content of L-th ...
Escherichia coli K-12
Biochim. Biophys. Acta
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1990
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Aronson
The primary structure of Esche ...
Escherichia coli K-12
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264
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1989
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Craig
Cd2+ activation of L-threonine ...
Escherichia coli K-12
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1988
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Interaction between L-threonin ...
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285714
Craig
L-Threonine dehydrogenase from ...
Escherichia coli K-12
Biochemistry
25
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1986
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1
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2
1
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1
2
-
-
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-
1
1
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-
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285716
Boylan
L-threonine dehydrogenase ...
Escherichia coli K-12
J. Biol. Chem.
256
1809-1815
1981
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-
-
-
-
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5
2
-
1
1
1
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4
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1
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1
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9
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1
1
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1
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1
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5
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2
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1
1
1
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1
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1
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9
-
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1
1
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285717
Aoyama
L-Threonine dehydrogenase of c ...
Gallus gallus
J. Biol. Chem.
256
12367-12373
1981
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-
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7
2
1
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2
1
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3
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1
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2
1
1
1
1
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1
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1
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1
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7
-
2
1
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2
1
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1
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2
1
1
1
1
-
-
-
-
1
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-
-
-
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-
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285718
Boylan
L-Threonine dehydrogenase of E ...
Escherichia coli K-12
Biochem. Biophys. Res. Commun.
85
190-197
1978
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-
-
-
-
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2
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1
1
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2
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1
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1
1
7
-
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1
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1
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1
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2
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1
1
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1
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1
1
7
-
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1
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-
285719
McGilvray
-
L-Threonine dehydrogenase (Art ...
Arthrobacter sp.
Methods Enzymol.
17B
580-584
1971
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-
-
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1
4
2
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3
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1
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1
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1
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1
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1
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1
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1
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1
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1
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4
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2
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3
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1
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1
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1
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1
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1
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285720
Green
The enzymatic formation of ami ...
Gallus gallus, Oryctolagus cuniculus, Rattus norvegicus, Staphylococcus aureus, Sus scrofa
biochem. J.
92
537-548
1964
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2
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5
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5
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1
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7
1
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5
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1
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1
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5
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5
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2
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5
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1
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7
1
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5
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1
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1
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285721
Hartshorne
Studies on liver threonine deh ...
Rana catesbeiana
Arch. Biochem. Biophys.
105
173-178
1964
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4
2
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1
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1
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1
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1
1
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1
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1
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1
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1
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4
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2
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1
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1
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1
1
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1
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1
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