Cloned (Comment) | Organism |
---|---|
into pET-30a digested with KpnIEcoRI to construct pET-ste11 and expressed in Escherichia coli BL21 | Streptomyces sp. 139 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ste11 gene knocked out with a double crossover via homologous recombination. Monosaccharide composition of exopolysaccharide produced by the mutant strain is altered from that of ebosin. Bioactivity of the mutant is lower than ebosin | Streptomyces sp. 139 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. 139 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by sonication, centrifugation and on a Ni-NTA resin column | Streptomyces sp. 139 |
Storage Stability | Organism |
---|---|
-20°C | Streptomyces sp. 139 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | - |
Streptomyces sp. 139 | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
additional information | ste11 gene encoding a TDH may function as a modifier gene of ebosin during its biosynthesis | Streptomyces sp. 139 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TDH | - |
Streptomyces sp. 139 |
threonine dehydrogenase | - |
Streptomyces sp. 139 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Streptomyces sp. 139 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | enzyme shows 50% activity at 25°C compared with that at 35°C | Streptomyces sp. 139 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptomyces sp. 139 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | activity at pH 5.5 is 50% of that at pH 7.5 | Streptomyces sp. 139 |