1.1.1.103: L-threonine 3-dehydrogenase

This is an abbreviated version, for detailed information about L-threonine 3-dehydrogenase, go to the full flat file.

Reaction

L-threonine
+
NAD+
=
L-2-amino-3-oxobutanoate
+
NADH
+
H+

Synonyms

CLOST_1621, L-ThrDH, L-threonine dehydrogenase, More, orf382, TDG, TDH, Thr dehydrogenase, ThrDH, threonine 3-dehydrogenase, threonine dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.103 L-threonine 3-dehydrogenase

Cloned

Cloned on EC 1.1.1.103 - L-threonine 3-dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned as a 3562-base pair EcoRI fragment
-
DNA and amino acid seuence determination and analysis, sequence comparisons, phylogenetic tree, expression of His-tagged enzyme in Escherichia coli strains JM109 and BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
gene tdh, functional expression in Escherichia coli
-
into pET-30a digested with KpnI–EcoRI to construct pET-ste11 and expressed in Escherichia coli BL21
-
ligated into the pTZ57R/T cloning vector. The recombinant pTZ-tdh plasmid digested with NcoI and EcoRI and the TDH gene ligated with pET-8c, giving the recombinant pET-tdh plasmid, used to transform Escherichia coli DH5alpha competent cells. Recombinant and purified pET-tdh plasmid expressed in Escherichia coli BL21 (DE3)
-
orf PH0655, amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain TOP10
-
orf PH0655, expression in Escherichia coli
-
orf PH0655, gene TDH, overexpression in Escherichia coli strain BL21(DE3)
-
orf PH0655, NA and amino acid sequence determination, analysis, and comparison, expression of selenomethionine-labeled wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
orf PH0655, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
overexpression in Escherichia coli
-
plasmid pTZ-tdh transformed in Escherichia coli DH5alpha competent cells. NcoI-EcoRI restriction fragment inserted into the pET-8c expression vector at the corresponding sites. The resulting plasmid pET-tdh expressed in Escherichia coli strain BL21(DE3)CodonPlus-RIL
-
recombinant pET-tdh plasmid expressed in Escherichia coli BL21 (DE3)
-