1.1.1.103: L-threonine 3-dehydrogenase

This is an abbreviated version, for detailed information about L-threonine 3-dehydrogenase, go to the full flat file.

Reaction

L-threonine
+
NAD+
=
L-2-amino-3-oxobutanoate
+
NADH
+
H+

Synonyms

CLOST_1621, L-ThrDH, L-threonine dehydrogenase, More, orf382, TDG, TDH, Thr dehydrogenase, ThrDH, threonine 3-dehydrogenase, threonine dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.103 L-threonine 3-dehydrogenase

Temperature Stability

Temperature Stability on EC 1.1.1.103 - L-threonine 3-dehydrogenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 75
quite stable at, rapid loss of activity above 80°C
25 - 35
-
85% inactivation after 2 min at 35°C, 10% inactivation at 35°C in the presence of 80 mM KCl
25 - 45
-
enzyme shows 50% activity at 25°C compared with that at 35°C
40
half-life 160 min
45
half-life 14 min
50
rapid inactivation
70
-
purified recombinant enzyme, 10 min, 90% activity remaining
80
-
60 min, pH 7.0, over 90% remaining activity, purified recombinant enzyme
85
-
the enzyme shows a half life of 2 h at 85°C and 24 min in boiling water
100
-
30 min, pH 7.0, over 90% remaining activity, purified recombinant enzyme
105
-
higher loss of activity above, purified recombinant enzyme
108
-
2-step thermal inactivation
120
-
20 min, inactivation, purified recombinant enzyme